Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Abstract

A three‐dimensional reduced graphene oxide porous aerogel with packaged TiO2 nanoparticles was prepared by a simple one‐step self‐assembly in a water bath at a mild temperature. Herein, we overcame the aggregation of graphene layers by forming three‐dimensional architecture, and utilized TiO2 aggregates as blocks to make the composite surface uneven. The extended aromatic areas contribute to DNA adsorption by aromatic stacking and hydrophobic interaction. The obtained composite exhibited porous structure and was positively charged at a low pH. The composite exhibited a high capacity of 890.1 mg g−1 and an adsorption efficiency of 98.4% to DNA at pH 3, however, bovine serum albumin at the same concentration level was hardly adsorbed. The composite showed favorable selectivity to DNA against protein even designed in such a simple manner. The retained DNA could be stripped by using a 0.04 mol L−1 Britton–Robinson buffer at pH 10, giving rise to a recovery of 96.3%. The DNA was isolated from human whole blood to be used as templates for PCR amplification successfully. This illustrates that DNA with high purity and quality was obtained.

The Comparing on 4-chlorophenyl Sulfoxide Modified the Reduced Graphene Oxide Composite for the Adsorption/Desorption of Pd (II) and Pt (IV) Ions

“…The D/G intensity ratio of the 4CS-rGO composite was 1.073, while the corresponding ratio of the GO was 0.938. It was consistent with previous papers [37][38][39] and the results of FT-IR spectra, illustrating that GO was reduced successfully.…”

One-Step Exfoliation/Etching Method to Produce Chitosan-Stabilized Holey Graphene Nanosheets for Superior DNA Adsorption

“…Therefore, the positively charged HG would be a perfect candidate for the delivery of negatively charged doublestranded plasmid DNA (pDNA) through the electrostatic interaction 9,40 coupled with the unique nanoporous structure. 8 Figure 6b and c displayed the adsorption behaviors of pDNA on nonporous graphene and HG, and HG showed a rapid and high DNA uptake capacity (the highest reported DNA uptake capacity, 1253 μg/mg until now 41 ). The superior high adsorption capacity was mainly due to the nanoporous structures of HG compared to the nonporous graphene, the electrostatic interaction, as well as the hydrophobic and πstacking interaction between nucleobases and the aromatic regions of graphene nanosheets.…”