Polycaprolactone-Based Scaffolds Facilitates Osteogenic Differentiation of Human Adipose-Derived Stem Cells in a Co-Culture System

Rozila, Ismail; Azari, Pedram; Sha'ban, Munirah; Safwani, Wan Kamarul Zaman Wan; Pingguan‐Murphy, Belinda; Chua, Kien Hui

doi:10.3390/polym13040597

Cited by 8 publications

(13 citation statements)

References 26 publications

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“…Comparing embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) are an attractive source of stem cell therapy that does not involve ethical concerns or tumorigenesis possibilities. MSCs, which are typically isolated from bone marrow and fat tissues, have a good ability to differentiate into adipocytes, osteoblasts, and chondrocytes ( Belka et al, 2019 ; Higuchi et al, 2019 ; Tsai et al, 2019 ; Albashari et al, 2020 ; Pan et al, 2020 ; Tan et al, 2020 ; Xing et al, 2020 ; Yang J. et al, 2020 ; El-Rashidy et al, 2021 ; Mohammed et al, 2021 ; Rozila et al, 2021 ) and show an ability to differentiate into not only these three types of cells but also ectoderm- or endoderm-derived cells ( Georgiou et al, 2015 ; Andrzejewska et al, 2019 ; Han et al, 2021 ; He et al, 2020 ; Luo et al, 2021a ; Luo et al, 2021b ; Van Rensburg et al, 2021 ; Wu et al, 2021 ; Zhu et al, 2021a ). For example, 5-azacytidine in cell culture medium induces MSC differentiation into cardiomyocytes and myoblasts ( Xu et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gao

Tian

Liu

et al. 2022

Front. Cell Dev. Biol.

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Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future.

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Section: Introductionmentioning

confidence: 99%

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gao

Tian

Liu

et al. 2022

Front. Cell Dev. Biol.

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“…Also, this finding of our study is consistent with Nanda et al, study 90 , 91 . When we compare our Alg/Cs/BG20 and Alg/Cs/Sr-BG20,30 scaffolds with well-known scaffolds such as PCL on new bone generation (calcium deposition and ALP), they have similar clinical potential to them 92 , 93 .…”

Section: Discussionmentioning

confidence: 99%

Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration

Manoochehri

Ghorbani

Moghaddam

et al. 2022

Sci Rep

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Repairing of large bone injuries is an important problem in bone regeneration field. Thus, developing new therapeutic approaches such as tissue engineering using 3D scaffolds is necessary. Incorporation of some bioactive materials and trace elements can improve scaffold properties. We made chitosan/alginate/strontium-doped bioglass composite scaffolds with optimized properties for bone tissue engineering. Bioglass (BG) and Sr-doped bioglasses (Sr-BG) were synthesized using Sol–Gel method. Alginate-Chitosan (Alg/Cs) scaffold and scaffolds containing different ratio (10%, 20% and 30%) of BG (Alg/Cs/BG10, 20, 30) or Sr-BG (Alg/Cs/Sr-BG10, 20, 30) were fabricated using freeze drying method. Characterization of bioglasses/scaffolds was done using zeta sizer, FTIR, XRD, (FE) SEM and EDS. Also, mechanical strength, antibacterial effect degradation and swelling profile of scaffolds were evaluated. Bone differentiation efficiency and viability of MSCs on scaffolds were determined by Alizarin Red, ALP and MTT methods. Cell toxicity and antibacterial effect of bioglasses were determined using MTT, MIC and MBC methods. Incorporation of BG into Alg/Cs scaffolds amplified biomineralization and mechanical properties along with improved swelling ratio, degradation profile and cell differentiation. Mechanical strength and cell differentiation efficiency of Alg/Cs/BG20 scaffold was considerably higher than scaffolds with lower or higher BG concentrations. Alg/Cs/Sr-BG scaffolds had higher mechanical stability and more differentiation efficiency in comparison with Alg/Cs and Alg/Cs/BG scaffolds. Also, Mechanical strength and cell differentiation efficiency of Alg/Cs/Sr-BG20 scaffold was considerably higher than scaffolds with various Sr-BG concentrations. Biomineralization of Alg/Cs/BG scaffolds slightly was higher than Alg/Cs/Sr-BG scaffolds. Overall, we concluded that Alg/Cs/Sr-BG20 scaffolds are more suitable for repairing bone major injuries.

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“…Upon compressive loading, all of the scaffolds exhibited comparable stress−strain curves (Figure 4), that is, the initial linear elastic region, also termed as Hookean region, followed by a plateau region and finally a region of densification. 10,33 The slope of the preliminary linear portion of the curve was used to calculate the compression modulus. 33 Similar behavior was also seen in other composites.…”

Section: Discussionmentioning

confidence: 99%

“…10,33 The slope of the preliminary linear portion of the curve was used to calculate the compression modulus. 33 Similar behavior was also seen in other composites. In a study by Seyedsalehi et al, 10 0.5 wt % rGO (reduced graphene oxide) with PCL showed good mechanical strength, but increasing the rGO concentration to 1 or 3 wt % resulted in a reduction in the strength.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Printing of Customized Scaffolds with Polycaprolactone–Silk Fibroin Composites and Integration of Gingival Tissue-Derived Stem Cells for Personalized Bone Therapy

Bojedla

Yeleswarapu

Alwala

et al. 2022

ACS Appl. Bio Mater.

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Regenerative biomaterials play a crucial role in the success of maxillofacial reconstructive procedures. Yet today, limited options are available when choosing polymeric biomaterials to treat critical size bony defects. Further, there is a requirement for 3D printable regenerative biomaterials to fabricate customized structures confined to the defect site. We present here a 3D printable composite formulation consisting of polycaprolactone (PCL) and silk fibroin microfibers and have established a robust protocol for fabricating customized 3D structures of complex geometry with the composite. The 3D printed composite scaffolds demonstrated higher compressive modulus than 3D printed scaffolds of PCL alone. Furthermore, the compressive modulus of PCL−Antheraea mylitta (AM) silk scaffolds is higher than that of the PCL− Bombyx mori (BM) silk scaffolds at their respective ratios. Compressive modulus of PCL−25AM silk scaffolds (73.4 MPa) is higher than that of PCL− 25BM silk scaffolds (65.1 MPa). Compressive modulus of PCL−40AM silk scaffolds (106.1 MPa) is higher than that of PCL−40BM silk scaffolds (77.7 MPa). Moreover, we have isolated, characterized, and integrated human gingival mesenchymal stem cells (hGMSCs), an effective autologous cell source, onto the 3D printed scaffolds to evaluate their bone regeneration potential. The results demonstrated that PCL−silk microfiber composite scaffolds of Antheraea mylitta origin showed much higher bioactivity than the Bombyx mori ones because of arginine−glycine−aspartic acid (RGD) sequences present in the Antheraea mylitta silk fibroin protein favoring cell attachment and proliferation. By day 14, the metabolic activity of hGMSCs was highest in PCL−40AM (4.5 times higher than that at day 1). In addition, to show the translational potential of this work, we have fabricated a patient defectspecific model (mandible) using the CT scan obtained by the micro-CT imaging to understand the printability of the composite for fabricating complex structures to restore maxillofacial bony defects with precision when applied in a clinical scenario.

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Polycaprolactone-Based Scaffolds Facilitates Osteogenic Differentiation of Human Adipose-Derived Stem Cells in a Co-Culture System

Cited by 8 publications

References 26 publications

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration

Three-Dimensional Printing of Customized Scaffolds with Polycaprolactone–Silk Fibroin Composites and Integration of Gingival Tissue-Derived Stem Cells for Personalized Bone Therapy

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