Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Abstract

Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future.

Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration model

“…F Line graph of the average amplitude of scotopic b wave at 0.01, 3.0, and 10.0 cd s/m 2 intensities for RCS rats in different groups at 8 weeks post-injection: non-cell injection (NONIJ, age-matched control), PBS injection, and cell injection of hDPSCs, hADSCs, hAFSCs, hBMSCs, hiPSCs, and hiPSC-RPE cells. *p < 0.05; "ns": the difference was not statistically significant CD73, and CD105 in four types of hMSCs (hAFSCs, hADSCs, hDPSCs, and hBMSCs), as well as the lack of expression of the human hematopoietic progenitor marker CD34 [48]. In addition, the human pluripotent markers Nanog, OCT4, and SSEA4 were detected in the hiPSC cell line HPS0077.…”

“…The hADSCs, hAFSCs, hBMSCs, and hDPSCs were MSCs, thus those cells were stained with antibodies against MSC markers (FITC-labeled anti-CD44 ( ] at the recommended concentrations using 5 µL (0.5 µg)/test, following a previously described method [48] for flow cytometry analysis of MSC markers on cells. The hiPSC-derived RPE cells were dissociated into single cells using 0.05% trypsin-EDTA phenol red (25200, Gibco, Grand Island, NY, USA) for 30 min at 37 °C.…”

A leptin-loaded poly-ϵ-caprolactone 3D printing scaffold for odontoblastic differentiation in human dental pulp cells

“…Leptin can induce the differentiation of dental pulp stem cells, including nerve, vascular, and periodontal ligament cells [47,55]. For leptin-adhered PCL to differentiate pulp cells efficiently, it must show sufficient elution at an appropriate rate [4,25,56].…”

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization

“…As can be seen from the results, the (+), (c), and (c/+) fractions could successfully synthesize a mineralized matrix, chondrocyte-typical proteins as well as neuronal cell markers. These are important characteristics of DPSCs, which are frequently reported in the literature [ 150 , 151 , 152 , 153 , 154 , 155 ]. The minor differences in the respective expression profiles might be relevant when aiming at the regeneration of a specific cell type.…”