Polycaprolactone-20% Tricalcium Phosphate Scaffolds in Combination With Platelet-Rich Plasma for the Treatment of Critical-Sized Defects of the Mandible: A Pilot Study

Rai, Bina; Ho, K.H.; Lei, Yang; Si-Hoe, Kuan-Ming; Teo, Choon-MengJeremy Jeremy; Yacob, Kamal bin; Chen, Fulin; Ng, Fooi-Chin; Teoh, Swee Hin

doi:10.1016/j.joms.2006.11.026

Cited by 69 publications

(49 citation statements)

References 24 publications

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“…Nonetheless, PCL-TCP scaffolds have been investigated for bone TE applications with reasonable success. 14,24,25 Inflammatory responses at the edges of the scaffold could still be determined with IBA-1-expressing microglial, and GFAP-expressing hypertrophic astrocytes. IBA-1 results indicated that PCL-TCP scaffolds activated fewer microglial cells initially (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Brain Tissue Interaction with Three-Dimensional, Honeycomb Polycaprolactone-Based Scaffolds Designed for Cranial Reconstruction Following Traumatic Brain Injury

Choy

Nga

Lim

et al. 2013

Tissue Engineering Part A

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Following traumatic brain injury (TBI), resultant voids are unable to support injections of suspension treatments, leading to ineffective healing. Moreover, without a structure to support the large defect, the defect site suffers from mechanical instability, which may impair the healing process. Therefore, having a delivery vehicle that can temporarily fill and provide mechanical support to the defect site may alleviate the healing process. In this work, we reported for the first time, the inflammatory response of brain tissue with polycaprolactone (PCL) and PCLtricalcium phosphate (TCP) scaffolds designed and fabricated for cranial reconstruction. After cranial defects were created in Sprague-Dawley rats, PCL and PCL-TCP scaffolds were implanted for a period of 1 week and 1 month. Following histology and immunofluorescence staining with the ionized calcium binding adaptor molecule-1 (IBA-1), glial fibrillary acidic protein (GFAP), nestin, and neuronal nuclei (NeuN), results indicated that IBA-1-positive activated microglia were observed across all groups, and declined significantly by 1 month ( p < 0.05). Interestingly, IBA-1-positive microglia were significantly fewer in the PCL-TCP group ( p < 0.05), suggesting a relatively milder inflammatory response. A decrease in the number of GFAP-positive cells among all groups over time ( > 29%) was also observed. Initially, astrocyte hypertrophy was observed proximal to the TBI site (55% in PCL and PCL-TCP groups, 75% in control groups), but it subsided by 1 month. Proximal to the TBI site, nestin immunoreactivity was intense during week 1, and which reduced by 1 month across all groups. NeuN-positive neurons were shrunken proximal to the TBI site ( < 0.9 mm), 32% smaller in the PCL-TCP group and 27% smaller in the PCL group. Based on above data indicating the comparatively milder, initial inflammatory response of brain tissue to PCL-TCP scaffolds, it is suggested that PCL-TCP scaffolds have notable clinical advantages as compared to PCL scaffolds.

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Section: Discussionmentioning

confidence: 99%

“…Over the years, our group has developed PCL and PCLtricalcium phosphate (PCL-TCP) as viable biomaterials for TE applications, including skin, 13 bone, [14][15][16] and drug delivery. 17,18 By using a solvent-free approach of fused deposition modeling, PCL-based scaffolds have been fabricated with a variety of porosities ranging from 70%-90%.…”

mentioning

confidence: 99%

Brain Tissue Interaction with Three-Dimensional, Honeycomb Polycaprolactone-Based Scaffolds Designed for Cranial Reconstruction Following Traumatic Brain Injury

Choy

Nga

Lim

et al. 2013

Tissue Engineering Part A

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“…233,253,254 One group has recently demonstrated enhanced osteoblast functionality in vitro and bone formation in vivo as a result of controlled delivery of calcium-phosphates and growth factors from PCL scaffolds. [255][256][257][258] In another novel approach, PCL scaffolds were functionalized with laminin-derived peptide sequences known to promote adhesion and proliferation (i.e., RGD, YIGSR). In vitro analysis with adipose-derived stem cells indicated that peptide grafting to PCL materials enhanced cellular adhesion and proliferation.…”

Section: Mechanisms Of Polymer Biodegradationmentioning

confidence: 99%

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Porter

Ruckh

Popat

2009

Biotechnology Progress

672

499

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Critical‐sized defects in bone, whether induced by primary tumor resection, trauma, or selective surgery have in many cases presented insurmountable challenges to the current gold standard treatment for bone repair. The primary purpose of a tissue‐engineered scaffold is to use engineering principles to incite and promote the natural healing process of bone which does not occur in critical‐sized defects. A synthetic bone scaffold must be biocompatible, biodegradable to allow native tissue integration, and mimic the multidimensional hierarchical structure of native bone. In addition to being physically and chemically biomimetic, an ideal scaffold is capable of eluting bioactive molecules (e.g., BMPs, TGF‐βs, etc., to accelerate extracellular matrix production and tissue integration) or drugs (e.g., antibiotics, cisplatin, etc., to prevent undesired biological response such as sepsis or cancer recurrence) in a temporally and spatially controlled manner. Various biomaterials including ceramics, metals, polymers, and composites have been investigated for their potential as bone scaffold materials. However, due to their tunable physiochemical properties, biocompatibility, and controllable biodegradability, polymers have emerged as the principal material in bone tissue engineering. This article briefly reviews the physiological and anatomical characteristics of native bone, describes key technologies in mimicking the physical and chemical environment of bone using synthetic materials, and provides an overview of local drug delivery as it pertains to bone tissue engineering is included. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009

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“…Previously, PCL-tricalcium phosphate scaffolds were developed and implanted into rat femoral defects. Histological evaluation illustrated infiltration of vascularized connective tissue and bone in treated rats, whereas the bone in-growth of untreated defects is minimal (Rai et al 2007a). Polycaprolactone-20% tricalcium phosphate (PCL-TCP) scaffolds were assessed for the treatment of critical-sized defects of the mandible.…”

Section: Polycaprolactone (Pcl)mentioning

confidence: 99%

Biomaterials Applicable for Alveolar Sockets Preservation: In Vivo and In Vitro Studies

Kunert‐Keil¹,

Gredes²,

Gedrange³

2011

Implant Dentistry - The Most Promising Discipline of Dentistry

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Polycaprolactone-20% Tricalcium Phosphate Scaffolds in Combination With Platelet-Rich Plasma for the Treatment of Critical-Sized Defects of the Mandible: A Pilot Study

Cited by 69 publications

References 24 publications

Brain Tissue Interaction with Three-Dimensional, Honeycomb Polycaprolactone-Based Scaffolds Designed for Cranial Reconstruction Following Traumatic Brain Injury

Brain Tissue Interaction with Three-Dimensional, Honeycomb Polycaprolactone-Based Scaffolds Designed for Cranial Reconstruction Following Traumatic Brain Injury

Bone tissue engineering: A review in bone biomimetics and drug delivery strategies

Biomaterials Applicable for Alveolar Sockets Preservation: In Vivo and In Vitro Studies

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