Polyaniline-polycaprolactone fibers for neural applications: Electroconductivity enhanced by pseudo-doping

Garrudo, Fábio F. F.; Mikael, Paiyz E.; Rodrigues, Carlos Alberto Chirano; Udangawa, Ranodhi N.; Paradiso, Patrizia; Chapman, Caitlyn A.; Hoffman, Pauline R.; Colaço, R.; Cabral, Joaquim M. S.; Morgado, Jorge; Linhardt, Robert J.; Ferreira, Frederico Castelo

doi:10.1016/j.msec.2020.111680

Cited by 26 publications

(35 citation statements)

References 74 publications

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“…The D.C. electrical conductivity of the composites was measured using the 4-point probe technique (Suss Manual 4 Probe station coupled to Keithley 4200 IV/CV meter, Tektronix, Beaverton, OR, USA). From previous tests, this equipment is able to measure electrical conductivities above 10 −7 S/m [35,36].…”

Section: Scaffold Design and Fabricationmentioning

confidence: 99%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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Understanding the mechano–biological coupling mechanisms of biomaterials for tissue engineering is of major importance to assure proper scaffold performance in situ. Therefore, it is of paramount importance to establish correlations between biomaterials, their processing conditions, and their mechanical behaviour, as well as their biological performance. With this work, it was possible to infer a correlation between the addition of graphene nanoparticles (GPN) in a concentration of 0.25, 0.5, and 0.75% (w/w) (GPN0.25, GPN0.5, and GPN0.75, respectively) in three-dimensional poly(ε-caprolactone) (PCL)-based scaffolds, the extrusion-based processing parameters, and the lamellar crystal orientation through small-angle X-ray scattering experiments of extruded samples of PCL and PCL/GPN. Results revealed a significant impact on the scaffold’s mechanical properties to a maximum of 0.5% of GPN content, with a significant improvement in the compressive modulus of 59 MPa to 93 MPa. In vitro cell culture experiments showed the scaffold’s ability to support the adhesion and proliferation of L929 fibroblasts (fold increase of 28, 22, 23, and 13 at day 13 (in relation to day 1) for PCL, GPN0.25, GPN0.5, and GPN0.75, respectively) and bone marrow mesenchymal stem/stromal cells (seven-fold increase for all sample groups at day 21 in relation to day 1). Moreover, the cells maintained high viability, regular morphology, and migration capacity in all the different experimental groups, assuring the potential of PCL/GPN scaffolds for tissue engineering (TE) applications.

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Section: Scaffold Design and Fabricationmentioning

confidence: 99%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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“…FT-IR analysis was performed to identify the chemical component of PVNF-n. As shown in Figure 2A , the FT-IR spectra of the PCL powder exhibited two characteristic peaks corresponding to the asymmetric/symmetric -CH 2 bands (2944cm −1 , 2,866 cm −1 ) and carbonyl groups (1720 cm −1 ) ( Garrudo et al, 2021 ). The VA powder exhibited two characteristic peaks corresponding to the stretching of the C–N band (1,413 cm −1 ) and N–H band (3,422 cm −1 ) ( Deng et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis

Tian

Zhou

Feng

et al. 2021

Front. Bioeng. Biotechnol.

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Site-specific release of therapeutics at the infected trachea remains a great challenge in clinic. This work aimed to develop a series of vancomycin (VA)-loaded polycaprolactone (PCL) composite nanofiber films (PVNF-n, n = 0, 1, and 5, respectively) via the electrospinning technique. The physiochemical and biological properties of PVNF-n were evaluated by a series of tests, such as FT-IR, XRD, SEM-EDS, and antibacterial assay. The PVNF-n samples displayed a typical network structure of fibers with random directions. VA was successfully introduced into the PCL nanofibers and could be sustained and released. More importantly, PVNF-5 showed relatively good antibacterial activity against both methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae (SPn). Thus, PVNF-5 was covered onto the self-expandable metallic stent and then implanted into a New Zealand rabbit model to repair tracheal stenosis. Compared to a metallic stent, a commercial pellosil matrix–covered stent, and a PVNF-0–covered metallic stent, the PVNF-5–covered airway stent showed reduced granulation tissue thickness, collagen density, α-SMA, CD68, TNF-α, IL-1, and IL-6 expression. In conclusion, this work provides an anti-infection film–covered airway stent that in site restrains tracheal stenosis.

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“…Some of them have been mixed with biopolymers to increase their biocompatibility and improve mechanical properties. 645,651,652 Mixtures of PANI with polycaprolactone have been extensively used in electrospinning for vasculature, 653 neural, 641,654,655 and osteogenic differentiation. 640,656 Stevens and co-workers developed electrospun block copolymers of aniline tetramer and PCL named AT-PCL.…”

Section: D Cell Culturesmentioning

confidence: 99%

Organic Bioelectronics for In Vitro Systems

et al. 2021

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Bioelectronics have made strides in improving clinical diagnostics and precision medicine. The potential of bioelectronics for bidirectional interfacing with biology through continuous, label-free monitoring on one side and precise control of biological activity on the other has extended their application scope to in vitro systems. The advent of microfluidics and the considerable advances in reliability and complexity of in vitro models promise to eventually significantly reduce or replace animal studies, currently the gold standard in drug discovery and toxicology testing. Bioelectronics are anticipated to play a major role in this transition offering a much needed technology to push forward the drug discovery paradigm. Organic electronic materials, notably conjugated polymers, having demonstrated technological maturity in fields such as solar cells and light emitting diodes given their outstanding characteristics and versatility in processing, are the obvious route forward for bioelectronics due to their biomimetic nature, among other merits. This review highlights the advances in conjugated polymers for interfacing with biological tissue in vitro, aiming ultimately to develop next generation in vitro systems. We showcase in vitro interfacing across multiple length scales, involving biological models of varying complexity, from cell components to complex 3D cell cultures. The state of the art, the possibilities, and the challenges of conjugated polymers toward clinical translation of in vitro systems are also discussed throughout.

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Polyaniline-polycaprolactone fibers for neural applications: Electroconductivity enhanced by pseudo-doping

Cited by 26 publications

References 74 publications

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

Vancomycin-Loaded Polycaprolactone Electrospinning Nanofibers Modulate the Airway Interfaces to Restrain Tracheal Stenosis

Organic Bioelectronics for In Vitro Systems

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