Polyaniline Nanowires-Based Electrochemical Immunosensor for Label Free Detection of Japanese Encephalitis Virus

Tuấn, Chu Văn; Huy, Trần Quang; Hieu, Nguyen Van; Tuan, Mai Anh; Trung, Tran

doi:10.1080/00032719.2012.755688

Cited by 26 publications

(13 citation statements)

References 25 publications

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“…From (2), the detection sensitivity was 0.024 ngmL −1 . This detection limit is substantially lower than the previously reported detection limit of 10 ngmL −1 for an interdigitated sensor [1] and a polyaniline nanowires based electrochemical immunosensor at below 10 ngmL −1 [5]. The CNPs modified SPCE biosensor reported in this work exhibited more than an order of magnitude higher in sensitivity as compared to the unmodified SPCE biosensor for JEV detection.…”

Section: Resultsmentioning

confidence: 42%

“…Early diagnosis is therefore critical in controlling JE outbreak and preventing the development of long-term serious symptoms in patients [3,4]. The conventional diagnostic methods for JEV include Enzyme-Linked Immunosorbent Assays (ELISA), Plaque Reduction Neutralisation Test, Reverse Transcription Polymerase Chain Reaction (RT-PCR), and virus isolation [1,2,5]. However, these methods require costly and bulky laboratory facilities, tedious diagnostic procedures, specially trained personnel, and long assay time.…”

Section: Introductionmentioning

confidence: 99%

“…Polyaniline nanowires modified interdigitated platinum (Pt) microelectrode electrochemical immunosensor with a detection limit of less than 10 ngml −1 has also been reported. Nonetheless, the interdigitated platinum (Pt) microelectrode used was not disposable, thus suffering a setback of crosscontamination problem [5].…”

Section: Introductionmentioning

confidence: 99%

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Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus

Lai

Chin

Pang

et al. 2017

Journal of Nanomaterials

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We reported a disposable and sensitive electrochemical biosensor strip based on carbon nanoparticles modified screen-printed carbon electrode (SPCE) for rapid and sensitive detection of Japanese Encephalitis Virus (JEV). Amino group functionalized carbon nanoparticles were prepared from preformed chitosan nanoparticles. Japanese Encephalitis Virus (JEV) antibody was immobilized onto the surfaces of carbon nanoparticles through amide bonds formation between amino groups of carbon nanoparticles and carboxylic groups of JEV antibody. The analytical performance of SPCE electrochemical biosensor strip was characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). SPCE electrochemical biosensor strip exhibited a linear detection range of 1-20 ngmL −1 with a low detection limit of 0.36 ngmL −1 (at S/N = 3) for JEV, detection sensitivity was 0.024 ngmL −1 for JEV, and analysis results were obtainable within 10 minutes. The potential clinical application of this SPCE electrochemical biosensor strip was demonstrated by the detection of JEV in human serum.

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Section: Resultsmentioning

confidence: 42%

Section: Introductionmentioning

confidence: 99%

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Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus

Lai

Chin

Pang

et al. 2017

Journal of Nanomaterials

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“…In recent times, biosensor-based methods have been envisaged as a promising tool for rapid detection of JEV antigens in outbreaks as well as for preliminary screenings and to recompense the conventional diagnostic methods (Tuan et al, 2013). In addition to that, they necessitate no costly chemicals and provide an outcome in few minutes.…”

Section: Biosensorsmentioning

confidence: 99%

“…In addition to that, they necessitate no costly chemicals and provide an outcome in few minutes. Serum antibody-based biosensors can detect up to the 1-10 g JEV antigens /ml with a detection limit of 0.75 g/ml (Huy et al, 2011) whereas electrochemical immunosensor developed using the APTES-GA-PrA-serum method (Tran et al, 2012) and Polyaniline nanowires (Tuan et al, 2013) detects the range between 25 ng ml−1 and 1µg ml−1 of JEV antigens, and normal detection limit was about 10 ng ml−1.…”

Section: Biosensorsmentioning

confidence: 99%

Japanese Encephalitis, Recent Perspectives on Virus Genome, Transmission, Epidemiology, Diagnosis and Prophylactic Interventions

Karthikeyan¹,

Shanmuganathan²,

Pavulraj³

et al. 2017

JEBAS

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Point‐of‐care detection of Japanese encephalitis virus biomarker in clinical samples using a portable smartphone‐enabled electrochemical “Sensit” device

Roberts

Dhanze

Sharma

et al. 2023

Bioengineering & Transla Med

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Japanese encephalitis (JE), a neglected tropical zoonotic disease prevalent in south‐east Asian and western pacific countries, caused by the flavivirus JE virus (JEV), has a dearth of electrochemical point‐of‐care (PoC) diagnostic tools available to manage endemic breakouts. To overcome this, we have developed a screen‐printed carbon electrode (SPCE) immunosensor for rapid PoC detection of JEV nonstructural 1 (NS1) antigen (Ag), found circulating in serum of infected individuals using a smartphone based portable “Sensit” device. The modification of SPCE surface with JEV NS1 antibody (Ab) was confirmed via observation of globular protein structures via scanning electron microscopy (SEM), increase in electrode surface hydrophilicity via contact angle measurement and decrease in current via differential pulse voltammetry (DPV). The fabrication and testing parameters were optimized based on highest current output obtained using DPV. The SPCE was tested for detection limit of target JEV NS1 Ag ranging from 1 fM to 1 μM, which was determined as 0.45 fM in spiked serum. The disposable immunosensor was also found to be highly specific in detecting JEV NS1 Ag over other flaviviral NS1 Ag. Finally, the modified SPCE was clinically validated by testing 62 clinical JEV samples using both a portable miniaturized electrochemical “Sensit” device coupled with a smartphone and a laboratory‐based potentiostat. The results were corroborated with gold‐standard RT‐PCR and showed 96.77% accuracy, 96.15% sensitivity, and 97.22% specificity. Hence, this technique may further be developed into a one‐step rapid diagnostic tool for JEV, especially in rural areas.

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Polyaniline Nanowires-Based Electrochemical Immunosensor for Label Free Detection of Japanese Encephalitis Virus

Cited by 26 publications

References 25 publications

Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus

Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus

Japanese Encephalitis, Recent Perspectives on Virus Genome, Transmission, Epidemiology, Diagnosis and Prophylactic Interventions

Point‐of‐care detection of Japanese encephalitis virus biomarker in clinical samples using a portable smartphone‐enabled electrochemical “Sensit” device

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