Polyamines

Tabor, Celia White; Tabor, Herbert

doi:10.1146/annurev.bi.53.070184.003533

Cited by 3,280 publications

(2,029 citation statements)

References 0 publications

Supporting

Mentioning

1,944

Contrasting

Unclassified

Order By: Relevance

“…This is reminiscent of the very sophisticated regulation of polyamine biosynthesis. Indeed, polyamines which are important modulators of cell proliferation and differentiation [23], are taken up by the cells similarly to histamine. It has been demonstrated that the activity of ornithine decarboxylase (ODC), the rate-limiting enzyme of the polyamine cascade, is regulated by intracellular polyamine concentrations in various ways, namely inhibition of translation, acceleration of ODC degradation and induction of an ODC-inhibitory protein named antizyme [24,25].…”

Section: S Corbel M Dy/febs Letters 391 (1996) 279-281mentioning

confidence: 99%

Evidence for bidirectional histamine transport by murine hematopoietic progenitor cells

Corbel

1996

FEBS Letters

View full text Add to dashboard Cite

Murine hematopoietic progenitor cells synthesize substantial amounts of histamine in response to IL-3 or calcium ionophore. They also take up extracellular histamine by an active transport system. In the present study we demonstrate that this system mediates both influx and efflux of histamine. Indeed, MR16155 and thioperamide, the two 1-13 antagonists which are most effective in inhibiting histamine uptake, likewise diminish the release of preloaded histamine from bone marrow cells. These compounds also inhibit the release of histamine which has been newly synthesized by hematopoietic progenitors in response to IL-3 or calcium ionophore, as assessed by the accumulation of the mediator inside the cells in the presence of the antagonists. The potency of different histamine receptor antagonists as inhihitors of histamine release increases with their capacity to block histamine uptake.Key words: Hematopoietic progenitor; Histamine; Bidirectional transport; Interleukin-3 Histamine uptake is temperature-dependent and partially requires sodium exchange [13]. Although we have provided evidence that this phenomenon is not mediated by classical histamine receptors, Ha receptor antagonists, such as thioperamide and MR16155, are potent inhibitors. Conversely to the polyamine transport system, which exists in numerous cell types and has been extensively studied [14,15], histamine transport seems to be restricted to astrocytes, endothelial and glial cells, and is still poorly understood [16][17][18]. It has been suggested that astrocytes could use the same means of transport for histamine uptake and release [18]. Herein, we provide evidence for a bidirectional transporter in bone marrow cells (BMC) and in the IL-3-dependent cell line FDCP1, as assessed by the finding that the inhibitors of histamine uptake likewise decrease release of preloaded histamine. We also show that this system is involved in the release of endogenous histamine which is newly synthesized in response to IL-3 or calcium ionophore since we found a decrease in histamine release accompanied by an increase in intracellular histamine contents in the presence of H3 receptor antagonists.

show abstract

Section: S Corbel M Dy/febs Letters 391 (1996) 279-281mentioning

confidence: 99%

Evidence for bidirectional histamine transport by murine hematopoietic progenitor cells

Corbel

1996

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Spermine is a tetravalent polyamine that is found in high concentrations in cells (1)(2)(3). It interacts with nucleic acids in diverse and important ways, functioning as a protective agent (4)(5)(6)(7)(8) and a modulator of DNA secondary structure (9).…”

Section: Introductionmentioning

confidence: 99%

Spermine Condenses DNA, but Not RNA Duplexes

Katz

Tolokh

Pabit

et al. 2017

Biophysical Journal

View full text Add to dashboard Cite

Interactions between the polyamine spermine and nucleic acids drive important cellular processes. Spermine condenses DNA and some RNAs, such as poly(rA):poly(rU). A large fraction of the spermine present in cells is bound to RNA but apparently does not condense it. Here, we study the effect of spermine binding to short duplex RNA and DNA, and compare our findings with predictions of molecular-dynamics simulations. When small numbers of spermine are introduced, RNA with a designed sequence containing a mixture of 14 GC pairs and 11 AU pairs resists condensation relative to DNA of an equivalent sequence or to 25 bp poly(rA):poly(rU) RNA. A comparison of wide-angle x-ray scattering profiles with simulation results suggests that spermine is sequestered deep within the major groove of mixed-sequence RNA. This prevents condensation by limiting opportunities to bridge to other molecules and stabilizes the RNA by locking it into a particular conformation. In contrast, for DNA, simulations suggest that spermine binds externally to the duplex, offering opportunities for intermolecular interaction. The goal of this study is to explain how RNA can remain soluble and available for interaction with other molecules in the cell despite the presence of spermine at concentrations high enough to precipitate DNA.

show abstract

“…This has led some researchers to suggest that polyamines mediate the action of plant hormones or are part of their signal response pathways [13]. In animal systems polyamine biosynthesis increases in response to hormones, and the polyamines produced are necessary for hormone action [14,15]. Since the early work of Slankis [16], phytohormones produced by the plant partner have been suspected to be involved in some of the morphological and/or anatomical modifications characteristic of the ectomycorrhizal association.…”

Section: Introductionmentioning

confidence: 99%

First‐report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment

et al. 1996

View full text Add to dashboard Cite

DFMO (~-DL-difluoromethylornithine), a specific irreversible inhibitor of ornithine decarboxylase (ODC), a polyamine biosynthetic pathway enzyme, strongly inhibits root growth and arbuscular mycorrhizal infection of Pisum sativum (P56 myc +, isogenic mutant of cv. Frisson). This inhibition is reversed when exogenous polyamine (putrescine) is included in the DFMO treatment, showing that the effect of DFMO on arbuscular mycorrhizal infection is indeed due to putrescine limitation and suggesting that ODC may have a role in root growth and mycorrhizal infection. However, treatment with gibbereHic acid (GA3) which increased root titers of polyamines strongly inhibited arbuscular mycorrhizal development. The possible role of polyamines in the regulation of the development of arbuscular mycorrhizal infection is discussed.

show abstract

Polyamines

Cited by 3,280 publications

References 0 publications

Evidence for bidirectional histamine transport by murine hematopoietic progenitor cells

Evidence for bidirectional histamine transport by murine hematopoietic progenitor cells

Spermine Condenses DNA, but Not RNA Duplexes

First‐report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment

Contact Info

Product

Resources

About