First‐report of the inhibition of arbuscular mycorrhizal infection of <i>Pisum sativum</i> by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment

Ghachtouli, Naïma El; Martin-Tanguy, J.; Paynot, M.; Gianinazzi, Silvio

doi:10.1016/0014-5793(96)00379-1

Cited by 102 publications

(48 citation statements)

References 35 publications

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“…Thus application of GA to mycorrhizal roots would be expected to phenocopy the della1/della2 mutant, and a dominant della mutant that is insensitive to GA but maintains function (55) would be expected to enable arbuscule formation even in the presence of GA. It has been shown previously that GA treatment suppresses arbuscule development in pea (41,72), although in these experiments the effects on the fungus and plant could not be easily distinguished. The application of GA 3 to M. truncatula roots inoculated with G. versiforme resulted in the expected alterations in plant growth and had a significant impact on development of symbiosis.…”

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confidence: 66%

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DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal symbiosis

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Lévy

Lévesque-Tremblay

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

253

267

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Most flowering plants are able to form endosymbioses with arbuscular mycorrhizal fungi. In this mutualistic association, the fungus colonizes the root cortex and establishes elaborately branched hyphae, called arbuscules, within the cortical cells. Arbuscule development requires the cellular reorganization of both symbionts, and the resulting symbiotic interface functions in nutrient exchange. A plant symbiosis signaling pathway controls the development of the symbiosis. Several components of the pathway have been identified, but transcriptional regulators that control downstream pathways for arbuscule formation are still unknown. Here we show that DELLA proteins, which are repressors of gibberellic acid (GA) signaling and function at the nexus of several signaling pathways, are required for arbuscule formation. Arbuscule formation is severely impaired in a Medicago truncatula Mtdella1/Mtdella2 double mutant; GA treatment of wild-type roots phenocopies the della double mutant, and a dominant DELLA protein (della1-Δ18) enables arbuscule formation in the presence of GA. Ectopic expression of della1-Δ18 suggests that DELLA activity in the vascular tissue and endodermis is sufficient to enable arbuscule formation in the inner cortical cells. In addition, expression of della1-Δ18 restores arbuscule formation in the symbiosis signaling pathway mutant cyclops/ipd3, indicating an intersection between DELLA and symbiosis signaling for arbuscule formation. GA signaling also influences arbuscule formation in monocots, and a Green Revolution wheat variety carrying dominant DELLA alleles shows enhanced colonization but a limited growth response to arbuscular mycorrhizal symbiosis.endosymbiosis | phytohormone | biotrophic | cyclops | Lotus japonicus

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confidence: 66%

“…that GA treatment inhibited the development of AM symbiosis and in particular arbuscule number (41).…”

mentioning

confidence: 89%

DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal symbiosis

Floß

Lévy

Lévesque-Tremblay

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

253

267

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“…Furthermore, GA has been shown to inhibit mycorrhizal colonization in Pisum sativum [2]. To understand the role of GA in this regulation, we sought to assess the function of GA during mycorrhizal colonization.…”

Section: Dear Editormentioning

confidence: 99%

A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants

et al. 2013

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“…The precise outcome depends on the concentration of GA applied. 12 Genes encoding enzymes of GA biosynthesis and metabolism are differentially regulated during symbiosis 7,[13][14][15][16][17] and associated with the colonized regions of the cortex. 18 Furthermore, both bioactive and inactive GAs are present in mycorrhizal roots.…”

Section: Introductionmentioning

confidence: 99%

DELLA proteins regulate expression of a subset of AM symbiosis-induced genes inMedicago truncatula

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Lévesque-Tremblay

Park

et al. 2016

Plant Signaling & Behavior

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The majority of the vascular flowering plants form symbiotic associations with fungi from the phylum Glomeromycota through which both partners gain access to nutrients, either mineral nutrients in the case of the plant, or carbon, in the case of the fungus. 1 The association develops in the roots and requires substantial remodeling of the root cortical cells where branched fungal hyphae, called arbuscules, are housed in a new membrane-bound apoplastic compartment.2 Nutrient exchange between the symbionts occurs over this interface and its development and maintenance is critical for symbiosis. Previously, we showed that DELLA proteins, which are well known as repressors of gibberellic acid signaling, also regulate development of AM symbiosis and are necessary to enable arbuscule development.3 Furthermore, constitutive overexpression of a dominant DELLA protein (della1-D18) is sufficient to induce transcripts of several AM symbiosis-induced genes, even in the absence of the fungal symbiont. 4 Here we further extend this approach and identify AM symbiosis genes that respond transcriptionally to constitutive expression of a dominant DELLA protein and also genes that do respond to this treatment. Additionally, we demonstrate that DELLAs interact with REQUIRED FOR ARBUSCULE DEVELOPMENT 1 (RAD1) which further extends our knowledge of GRAS factor complexes that have the potential to regulate gene expression during AM symbiosis. Development of arbuscular mycorrhizal (AM) symbiosis involves signal exchange between the symbionts and a highly conserved plant symbiosis signaling pathway plays a central role in controlling fungal growth into the root and the development of the symbiotic interface in the cortical cells. 5,6 The gene expression changes that underlie symbiotic development have been documented through detailed transcript profiling 7,8 and some transcriptional regulators have been identified, some of which are downstream components of the symbiosis signaling pathway. 4,[9][10][11] Development of AM symbiosis is also influenced by plant hormones and treatment of roots with gibberellic acid (GA) leads either to alterations in fungal entry into the root or abolishes arbuscule development. The precise outcome depends on the concentration of GA applied.12 Genes encoding enzymes of GA biosynthesis and metabolism are differentially regulated during symbiosis 7,13-17 and associated with the colonized regions of the cortex.18 Furthermore, both bioactive and inactive GAs are present in mycorrhizal roots. All of these data suggest a role for GAs in regulating aspects of symbiotic development. 18,19 DELLA proteins are members of a specific sub-group of the GRAS family of transcriptional regulators that possess a unique domain, the DELLA domain, located toward the N-terminal end of the protein.20 DELLAs function as repressors of GA signaling and their mechanism of action has been elucidated through extensive studies in Arabidopsis. Briefly, in the presence of GA, the GA receptor (GID) interacts with the DELLAs through ...

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First‐report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment

Cited by 102 publications

References 35 publications

DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal symbiosis

DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal symbiosis

A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants

DELLA proteins regulate expression of a subset of AM symbiosis-induced genes inMedicago truncatula

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