Poly(sodium-10-Undecylenate) as Pseudo-Stationary Phase in the Separation and Quantification of Polyaromatic Hydrocarbons by Micellar Electrokinetic Chromatography

Abstract

In this work we reported the synthesis of different molar mass fraction of poly(sodium-10-undecylenate) (PSU), and its subsequent utilization as pseudostationary phase in the separation of a mix of 4 polyaromatic hydrocarbons (PAHs); naphthalene (NAPH), fluorene (FLU), phenantrene (PHEN) and pyrene (PYR). Concentration and molar mass fraction of PSU were the main variable studied. The analytical parameters; efficiency (N), selectivity (α), and resolution (R s ) were calculated to assess the separation capacity. The best conditions for the separation were achieved using a buffer composed by 12.5 mM of each sodium borate and sodium hydrogen phosphate, yielding pH 9.2 in ACN/H 2 O (40/60) solvent, using PSU of molar mass fraction 30-50 kDa in concentration of 0.25 g 100 mL -1 . The applied voltage was 25 kV. Finally, Limit of Detection (LOD) and Limit of Quantification (LOQ) for 4 PAHs were calculated from the calibration curves in optimal separation conditions.