Poly(o-phenylenediamine) electrosynthesized in the absence of added background electrolyte provides a new permselectivity benchmark for biosensor applications

Rothwell, Sharon A.; Killoran, Sarah J.; Neville, Elaine M.; Crotty, Aoife M.; O’Neill, Robert

doi:10.1016/j.elecom.2008.05.018

Cited by 28 publications

(32 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…82 Implanted Pt C -based biosensors, however, sample the ECF around a relatively large population of cells, and smaller designs are needed both to reduce tissue damage (by reducing sensor diameter), and to study small brain regions or layers of cells within regions, for example, by using disk sensors. In this study, we fabricated Pt/PPD electrodes of different sizes and geometries to determine their sensitivity to H 2 O 2 and to the archetypal interference species, AA.…”

Section: Resultsmentioning

confidence: 99%

Contributions by a Novel Edge Effect to the Permselectivity of an Electrosynthesized Polymer for Microbiosensor Applications

et al. 2009

Self Cite

View full text Add to dashboard Cite

Pt electrodes of different sizes (2 × 10 -5 -2 × 10 -2 cm 2 ) and geometries (disks and cylinders) were coated with the ultrathin non-conducting form of poly(o-phenylenediamine), PPD, using amperometric electrosynthesis. Analysis of the ascorbic acid (AA) and H 2 O 2 apparent permeabilities for these Pt/PPD sensors revealed that the PPD deposited near the electrode insulation (Teflon or glass edge) was not as effective as the bulk surface PPD for blocking AA access to the Pt substrate. This discovery impacts on the design of implantable biosensors where electrodeposited polymers, such as PPD, are commonly used as the permselective barrier to block electroactive interference by reducing agents present in the target medium. The undesirable "edge effect" was particularly marked for small disk electrodes which have a high edge density (ratio of PPDinsulation edge length to electrode area), but was essentially absent for cylinder electrodes with a length of >0.2 mm. Sample biosensors, with a configuration based on these findings (25 µm diameter Pt fiber cylinders) and designed for brain neurotransmitter L-glutamate, behaved well in vitro in terms of Glu sensitivity and AA blocking.The design of biosensors for implantation in functioning biological tissues is an important area of research with significant socioeconomic impact. 1-4 Depending on the target analyte, different signal transduction pathways have been exploited in the design of enzyme-based biosensors, including direct oxidation of reduced oxidases, 5-7 dehydrogenase chemistries, 8-10 redox mediators, 11-13 and spectrophotometric approaches. 14-16 However, "first generation" electrochemical biosensors, based on reactions 1-3, remain the most common design for many applications. 1,2,[17][18][19] The majority of these are oxidase-based devices designed to operate in amperometric mode, detecting H 2 O 2 generated by the reaction of the co-substrate (dioxygen) with the reduced form of the enzyme.First-generation biosensors in general, and especially those designed for tissue implantation, 20-24 must be capable of effective rejection of electroactive interference by endogenous reducing agents in the target medium because these devices are normally operated at a high applied overpotential for efficient H 2 O 2 * To whom correspondence should be addressed. E-mail: robert.oneill@ucd.ie.

show abstract

Section: Resultsmentioning

confidence: 99%

Contributions by a Novel Edge Effect to the Permselectivity of an Electrosynthesized Polymer for Microbiosensor Applications

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…1b) and were separated into single units when required. The area of the working electrode utilized in this study is 12.96 mm 2 , (see Fig. 1a, working electrode 1) and it should be noted here that only the working electrodes undergo polymerization (see Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Since the first report on the polymerization of o-phenylenediamine (oPD) on conducting substrates via electrochemical oxidation [1], a number of papers have appeared on possible applications of the resultant polymer films. Recently, PoPDA has been utilized as permselective membrane in biosensor design [2], a surface modifying agent to facilitate immunospecies detection [3] and as a component in rechargeable cells [4]. Previous work has explored a new possible application for PoPDA in the field of sonochemically fabricated microelectrode arrays [5].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Poly(o‐phenylenediamine) Films for Application as Insulating Layers upon Carbon Substrates for Use within Sonochemically Fabricated Microelectrode Arrays

Gornall

Collyer²,

Higson

2010

Electroanalysis

View full text Add to dashboard Cite

The formation of insulating layers of poly(o-phenylenediamine) via electropolymerization was performed on single carbon screen-printed electrodes. The effects of experimental parameters on the properties of the film were investigated, allowing for technique optimization. These conditions were then used to deposit films upon surfaces of 100 interconnected electrodes, with cyclic voltammetry used to study the electroactivity of the resulting electrodes. The insulating property of the film, for use in the formation of microelectrode array amperometric sensors, was evaluated. Finally, the insulated sensors were exposed to ultrasonic ablation to form microelectrode arrays, and these were subsequently assessed using optical and electrochemical techniques.

show abstract

“…In various studies, working electrodes were coated by poly(o-phenylenediamine) film using aqueous solution of monomer as an outer layer for elimination interferences. 38,50 In cholesterol biosensors, poly(o-phenylenediamine) films were also used for elimination interference and determination of cholesterol in human serum could not be performed by using prepared biosensor. 12,51 However, in the present study, poly(ophenylenediamine) film is composed both permselective membrane and immobilization matrix for enzyme.…”

Section: (1)mentioning

confidence: 99%

“…Ortho isomer poly(o-phenylenediamine) of polyphenylenediamines is the most studied form, and is the subject of the present study. 38 Poly(o-phenylenediamine) films have been widely used on amperometric glucose, 26,30,[39][40][41][42][43][44][45] phenol, 46 L-lysine 47,48 and ethanol 49 biosensor for elimination of interference from electroactive species present in real samples. A literature search revealed that there were no reports of biosensor prepared by the polymerization conditions used in this study.…”

Section: (1)mentioning

confidence: 99%

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Zeybek¹,

Zeybek²,

Pekmez³

et al. 2012

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Um eletrodo enzimático amperométrico baseado em polímero foi preparado para detecção amperométrica de colesterol livre. Inicialmente, o filme polimérico poli(o-fenilenodiamina) (PoPD) foi eletropolimerizado no meio acetonitrila-água contendo o monômero o-fenilenodiamina (oPD) e ácido canforsulfônico em eletrodo de Pt através da técnica de voltametria cíclica. A enzima oxidase colesterol foi imobilizada sobre a superfície do eletrodo Pt/PoPD. A determinação de colesterol foi realizada por maio do monitoramento da corrente de oxidação de H 2 O 2 produzido enzimaticamente a +0,7 V vs. Ag/AgCl. A concentração da solução tampão, o valor de pH e a temperatura de trabalho otimizados foram 0,05 mol L −1 , 7,5 and 40 °C, respectivamente. O intervalo de trabalho do eletrodo de enzima para colesterol foi 9,8 × 10 −3 -11 µmol L −1 e tempo de resposta 150 s. Os efeitos de possíveis interferências presentes em amostras de soro em resposta ao eletrodo de enzima foram examinados. A determinação do colesterol total em amostras de soro foi realizada usando o eletrodo de enzima Pt/PoPD/ChOx proposto e os resultados foram em bom acordo com os obtidos pelo método espectrofotométrico.A polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (±)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H 2 O 2 at +0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L −1 , 7.5 and 40 °C, respectively. The working range of enzyme electrode to cholesterol was 9.8 × 10 −3 -11 µmol L −1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.

show abstract

Poly(o-phenylenediamine) electrosynthesized in the absence of added background electrolyte provides a new permselectivity benchmark for biosensor applications

Cited by 28 publications

References 41 publications

Contributions by a Novel Edge Effect to the Permselectivity of an Electrosynthesized Polymer for Microbiosensor Applications

Contributions by a Novel Edge Effect to the Permselectivity of an Electrosynthesized Polymer for Microbiosensor Applications

Evaluation of Poly(o‐phenylenediamine) Films for Application as Insulating Layers upon Carbon Substrates for Use within Sonochemically Fabricated Microelectrode Arrays

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Contact Info

Product

Resources

About