Poly (ethylene-co-vinyl alcohol)-based polymeric thermo-responsive nanocarriers for controlled delivery of epirubicin to hepatocellular carcinoma

Abstract

In this study, poly(ethylene-co-vinyl alcohol) (EVOH) as a novel biocompatible polymeric scaffold was surface modified by succinylation to get EVOHS and further pegylated to improve structural properties using methoxypolyethylene glycol (5000 Da) succinate (PEGS) along with targeting with retinoic acid (RA) to get final modified active and passive targeted conjugate (PEGS-EVOHS-RA) to evaluate its ability in carrying and delivery of epirubicin to hepatocellular carcinoma cell lines in response to varying temperatures. In this regard, the PEGS-EVOHS-RA conjugate was prepared through the desired chemical reactions and its structure was confirmed using 1H-NMR and FT-IR spectra. The micelles were prepared from PEGS-EVOHS-RA by dialysis method. The Particle size and zeta potential were measured, and entrapment efficacy along with in vitro release efficiency in different temperatures were also studied. The structural morphology of optimized nanomicelle was studied by transmission electron microscopy micrographs. The desired final micelles were evaluated for their toxicity using MTT assay on HepG2 human hepatocellular carcinoma cell lines at normal (37 °C) and elevated temperature (45 °C). The results revealed that, as the hydrophilicity of micelles increased, all characteristic properties improved. Then, these micelles can be considered as potentially effective thermo responsive delivery systems for targeted delivery of cytotoxic agents to hepatocellular carcinoma.

Aerosol characterisation of nebulised liposomes co-loaded with erlotinib and genistein using an abbreviated cascade impactor method

“…A p value < 0.05 was considered significant. (Hassanzadeh et al, 2017;Mahajan and Mahajan, 2016). Furthermore, the smaller particle size of nanocarriers ensures efficient incorporation into droplets during aerosol generation (Amini et al, 2014;Dandekar et al, 2010).…”

Preparation and Characterization of Poly(ethylene-co-vinyl alcohol)/poly(ε-caprolactone) Blend for Bioscaffolding Applications

“…This copolymer is synthesized by the polymerization of ethylene with vinyl acetate giving the poly(ethylene-co-vinyl acetate) following the hydrolysis of functional groups. E-VAL is also biodegradable [17], biocompatible [18][19][20][21], and possesses excellent mechanical and gas barrier properties. Few investigations have been reported on the application of this copolymer in the biomedical domain.…”

“…Matsumura et al [20] demonstrated the control of the differentiation and proliferation of the periodontal cells used to develop a highly organized hybrid implant. Hassanzadeh et al [21] reported on the surface modification of E-VAL by succinylation to obtain succinate modified E-VAL (EVOHS) to improve the structural properties and evaluated its ability to deliver epirubicin to hepatocellular carcinoma cells in response to varying temperature. They revealed that all the characteristic properties improved as the hydrophilicity of micelles increased, and these entities can be effective as thermo responsive delivery systems for targeting the delivery of cytotoxic agents to hepatocellular carcinoma.…”

Enhanced water and oxygen barrier performance of flexible polyurethane membranes for biomedical application

“…As one can see from Figure 2A, the spectrum of uncovered CFAR‐LT membrane presents all characteristic PU bonds, namely the symmetric N‐H stretching vibrations of the urethane groups near 3313 cm −1 ; the in‐plane N–H bending vibration of the isocyanate segments at 1593 cm −1 ; the stretching vibration of the carbonate groups C=O and the asymmetric stretching vibration of C–O (from N–CO–O) at 1741 and 1251 cm −1 , respectively; the C‐O‐C bond stretching vibration in the urethane group at 970 cm −1 5,13,34. The absorption peak at around 1635 cm −1 corresponds to the bonded carbonyl group 5,24,35 and indicates that a diamine was used as the chain extender in this case. A better phase separation of the hard and soft segments in case of CFAR‐LT testifies to a higher mobility of the macromolecular chains, thus promoting the diffusion of the small molecular species 13 .…”

“…It is also known that EVOH is a biocompatible material 9,20 . For example, Hassanzadeh et al show that polymer micelles based on EVOH modified by succinylation and further pegylated can be successfully used for the epirubicin delivery for the treatment of hepatocellular carcinoma 24 . The toxicity of such modified micelles was tested on HepG2 human hepatocellular carcinoma cell line using 3‐[4,5‐dimethylthriazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide assay as a function of temperature (up to 45°C).…”