Aerosol characterisation of nebulised liposomes co-loaded with erlotinib and genistein using an abbreviated cascade impactor method

Abstract

Erlotinib and genistein co-loaded liposomes were prepared by the thin-film hydration method. The effect of probe sonication as a size reduction method on drug incorporation and the properties of aerosols generated using air-jet and vibrating-mesh nebulisers was studied. The use of the Next Generation Impactor (NGI) to characterise inhaler formulations is limited by the need accurately to quantify drug deposited across 8 stages and is labour intensive to use. The Fast Screening Impactor (FSI) comprising two impaction stages was compared with the NGI to evaluate its applicability as a simple screening and labour-saving tool to characterise nebulised systems. For the developed liposomal formulations, an air-jet nebuliser generated a two-fold higher fine particle fraction (FPF) than a vibrating-mesh nebuliser. The findings demonstrated that the cooled FSI (5°C) operated at 15 L/min was effective in differentiating the aerosol properties of the nebulised liposome formulations investigated. Overall, the optimised co-loaded liposomes were more effectively delivered by an air-jet nebuliser, than from a vibrating-mesh nebuliser over a 10 min period as determined using the abbreviated impactor.

Fragrance Component Analysis for Nebulvapours of European Anchovy Oils by Using Colorimetric Printing and Electronic Nose

“…Optimized loaded liposomes were more delivered effectively by an air-jet nebulizer, than a vibrating-nebulizer during 10 min period via the abbreviated impactor in a study [31]. Similar to this conclusion, in this study, with applying in parallel of jet nebulizer, anchovy oil vapors were sent on tela fabric surface in effective rate successfully.…”

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

“…PARI VELOX ® represents vibrating-mesh nebulisation and PARI BOY ® SX air-jet nebulisation. Vibrating-mesh nebulisers extrude liquid through a perforated mesh to generate aerosols, whereas air-jet nebulisers use compressed air to press the liquid through a nozzle of defined size ( Figure 1 a,b) [ 39 , 40 ].…”

“…In the recent literature the two nebulisation techniques are described to function with nanoscaled drug carrier systems [ 44 , 45 ] and are suitable for therapies with small volumes since both require a minimum sample volume of only 2 mL. They provide comparable lung deposition profiles and show reportedly no damage of precursor liposomes [ 39 , 45 ]. A comparison of the measured size values for both nebulisation techniques of all formulations ( Table 2 ) revealed no significant difference ( p > 0.05) between PARI VELOX ® and PARI BOY ® SX.…”

“…One focus in the course of drug nebulisation was the application of liposomes [ 39 , 46 ]. State of the art in pulmonary drug delivery for the treatment of lung cancer is the nebulisation of different nanocarriers such as nanoparticles, nanoemulsions and liposomes [ 47 , 48 , 49 , 50 ].…”

Antioxidant Activities, Phenolic Contents and Electronic Nose Analysis of Black Garlic

“…Collected extraction oils of black garlic were placed in the chamber of nebulizer device (particle size <4 µm, Bayer company, Germany). Components analysis of obtained nebulous vapors were realized with electronic nose (PERES foodsniffer (Swiss Technology)) including volatile organic compound sensors combined smartphone (Samsung Galaxy S5 (Seoul, South Korea)) network library in a short time (120 second) [21,22] (Figure 2).…”