Pollen Viability and Germinability of Gamma Irradiated M4 Lines of Sesame

Audu, M.A.; Falusi, O. A.; Muhammad, Mirza; Daudu, O. A. Y.; Abubakar, A.

doi:10.35849/bjare202102002

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Sesame (Sesamum indicum L.) is one of the most important oil crops. Gamma irradiation is a reasonable tool to induce variability in sesame and is advantageous in increasing pollen viability (Audu et al, 2021;Ryu, Doo & Kim, 1992).…”

Section: Haploid Methods Used In Oilseed Plantsmentioning

confidence: 99%

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Yaman

Sanyürek

2023

Biotech Studies

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Haploid plant production is of great importance to shorten the breeding period in plant breeding programs. Obtaining pure lines in plant growing programs require an intensive work with huge labor and time. Obtaining one hundred percent homozy-gous pure lines is a key point for the improvement and development of new cultivars. Haploid plants with a single set of homozygous chromosomes have become a valua-ble tool in plant breeding. Dihaploid plants that are homozygous at all loci with dou-bling of their chromosomes can be propagated by seed and reach full homozygosity in a single generation. Traditional methods take seven years to reach homozygosity. Dihaploidization methods provide significant advantages in terms of gaining homo-zygosity in a short period of one year and bringing pure lines into agriculture. Anther culture and irradiated pollen technique are among the most widely used techniques in this respect; where physical or chemical agents are used to induce mutated pollen grains and anthers that are subsequently employed to develop dihaploids through in vitro cultures. These techniques are a good source to facilitate gene mapping, cyto-genetic research, and evolutionary studies. Irradiated pollen culture techniques have been applied to many oilseed crops to obtain pure lines. This study highlights some salient features of producing dihaploids using irradiated pollen grains and their maintenance.

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Section: Haploid Methods Used In Oilseed Plantsmentioning

confidence: 99%

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Yaman

Sanyürek

2023

Biotech Studies

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Influence of Gamma Irradiation on Pollen Viability, Pollen Tube Growth, and Fruit Development in Tomato (<i>Solanum lycopersicum</i> L.)

Kantoğlu

2024

Hortic J

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The goal of this study was to assess whether irradiated pollen technology could be used in tomato breeding research. The effects of irradiation on pollen viability, fruit set rate, and embryo formation were investigated. For this purpose, pollens were exposed to gamma rays of 0, 50, 100, 200, 300, and 400 Grays (Gy). The effect of irradiation on pollen viability and tube growth was found to be significant based on counting and measurements performed under in vitro conditions at 24, 48, 72, and 144 h after irradiation. Fruit set and embryo formation in seeds were evaluated 30 days after pollination with pollen irradiated at different doses. It was determined that increasing the irradiation dose resulted in reduced pollen viability and tube length. Endosperm formation was detected in all seeds after 50 Gy of irradiation. However, 50 Gy had no effect on gynogenesis stimulation. Therefore, 100, 200, and 300 Gy doses stimulated embryo formation without endosperm, while 400 Gy of irradiated pollen did not support fruit to set. These results indicate the importance of harvesting time to obtain viable embryos. It should be retracted to an earlier time since late harvest resulted in necrosis of globular embryos on the 30 th day after irradiation. Pollination with one-day-old irradiated pollen was more suitable for gynogenesis induction. The results showed that the irradiated pollen technique can be applied in tomato breeding studies, especially in terms of purifying the obtained breeding lines in a shorter time. In particular, determining the appropriate induction dose for gynogenesis depending on the genotype is important for stimulation efficiency.

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Pollen Viability and Germinability of Gamma Irradiated M4 Lines of Sesame

Cited by 2 publications

References 16 publications

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Obtaining haploid plants by irradiated pollen culture in oil seed crops

Influence of Gamma Irradiation on Pollen Viability, Pollen Tube Growth, and Fruit Development in Tomato (<i>Solanum lycopersicum</i> L.)

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