Pollen allergen skin test and specific IgE reactivity among Filipinos: a community-based study

Sabit, Maureen; Wong, Cecil; Andaya, Agnes; Ramos, John Donnie A.

doi:10.1186/s13223-020-00471-9

Cited by 9 publications

(6 citation statements)

References 47 publications

(56 reference statements)

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“…In recent years, allergic diseases caused by pollens have attracted much attention, especially in the plant species releasing pollens, specific IgE reactivity and the influence of air pollutants on pollen transmission [ 23 – 25 ]. Nevertheless, identification of allergenic proteins and their bioactivity have remained elusive.…”

Section: Discussionmentioning

confidence: 99%

Analysis of allergen components and identification of bioactivity of HSP70 in pollen of Populus deltoides

et al. 2021

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Background Allergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear. Methods The total proteins in pollen of P. deltoides were analyzed by proteomics, and the potential allergens were identified via the WHO/IUIS database and the allergenOnline database retrieval. One target protein was screened by bioinformatics and expressed in Escherichia coli. The biological activity of the expressed product was verified by animal experiments. Results The total of 3929 proteins in pollen of P. deltoides were identified, and 46 potential allergens belonging to 10 protein families were recognized by database retrieval. B9N9W6 protein of Hsp70 family was screened by bioinformatics analysis and expressed successfully. ELISA showed that B9N9W6 can stimulate the immune system to produce specific IgE and promote the generation of IL-4. Flow cytometry showed that B9N9W6 can significantly stimulate the proliferation of CD4+ T cells and promote the polarization of Th2 cells. The pathological sections of mice lung tissues indicated that alveolar destruction was more severe in the B9N9W6 group than that of extract group, and there were more inflammatory cells infiltration, mucus exudation and bleeding. Conclusion B9N9W6 is an important antigenic substance in the pollen of P. deltoides. Due to the conserved structure of Hsp70 family, more attention should be paid to the possibility of sensitization when Hsp70 from any pathogenic species is administered.

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Section: Discussionmentioning

confidence: 99%

Analysis of allergen components and identification of bioactivity of HSP70 in pollen of Populus deltoides

et al. 2021

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“…A recent study in Philippines using tree and grass pollen extracts to perform SPT in 130 patients with AAR showed that 63.85% were sensitized to mango (Mangifera indica), followed by Japanese acacia (Acacia auriculiformis, 27.69%), sensitive plant (Mimosa spp., 25.38%), spiny amaranth (Amaranthus spinosus, 21.54%), prickly lantana (Lantana camara, 20.0%). However, the correlations between SPT results and sIgE antibodies to those extracts were weak [57]. The prevalence of pollen sensitizations evaluated using available commercial allergen extracts in other studies were much lower and could not reflex the accurate prevalence in Southeast Asia due the different pollen profiles in Southeast Asian countries from those of other areas.…”

Section: Allergic Rhinitis Prevalence In Southeast Asiamentioning

confidence: 82%

Characteristics of allergen profile, sensitization patterns and Allergic Rhinitis in SouthEast Asia

Pham

Trinh

et al. 2022

Current Opinion in Allergy &Amp; Clinical Immunology

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Purpose of reviewTo highlight the characteristics of allergic rhinitis, local allergic rhinitis (LAR), and importance of allergens in Southeast Asian countries. Recent findingsThe Asia-Pacific region is very diverse with disparities in the epidemiological data between countries as well as in the unmet needs. The prevalence of allergic rhinitis has markedly increased in the past decades, with a high variation between countries, ranging from 4.5-80.3%. In terms of LAR, the reported prevalence in Southeast Asia is similar to that of other Asian countries (3.7-24.9%) but lower than that in western countries. House dust mites, cockroach, pollens, and molds are major allergens that are known triggers for of allergic rhinitis in this region, whereas the association with helminth infection requires further investigation.

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“…In recent years, allergic diseases caused by pollens have attracted much attention, especially in the plant species releasing pollens, speci c IgE reactivity and the in uence of air pollutants on pollen transmission [21][22][23]. Nevertheless, identi cation of allergenic proteins and their bioactivity have remained elusive.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Allergen Components and Identification of Bioactivity of HSP70 in Pollen of Populus Deltoides

Wei

Zhan

Jiang

et al. 2021

Preprint

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Background: Allergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear. Methods: The total proteins in pollen of P. deltoides were analyzed by proteomics, and the potential allergens were identified via the WHO/IUIS database and the allergenOnline database retrieval. The target protein was screened by bioinformatics and expressed in Escherichia coli. The biological activity of the expressed product was verified by animal experiments. Results: 3929 total proteins in pollen of P. deltoides were identified, and 49 potential allergens belonging to 10 protein families were recognized by database retrieval. B9N9W6 protein of Hsp 70 family was screened by bioinformatics analysis and expressed successfully. ELISA showed that B9N9W6 can stimulate the immune system to produce specific IgE and promote the generation of IL-4. Flow cytometry showed that B9N9W6 can significantly stimulate the proliferation of CD4+ T cells and promote the polarization of Th2 cells. The pathological sections of mice lung tissues indicated that alveolar destruction was more severe in the B9N9W6 group than that of extract group, and there were more inflammatory cells infiltration, mucus exudation and bleeding. Conclusion: B9N9W6 is an important antigenic substance in the pollen of P. deltoides. Due to the conserved structure of Hsp 70 family, more attention should be paid to the possibility of sensitization when HSP 70 from any pathogenic species is administered.

show abstract

Pollen allergen skin test and specific IgE reactivity among Filipinos: a community-based study

Cited by 9 publications

References 47 publications

Analysis of allergen components and identification of bioactivity of HSP70 in pollen of Populus deltoides

Analysis of allergen components and identification of bioactivity of HSP70 in pollen of Populus deltoides

Characteristics of allergen profile, sensitization patterns and Allergic Rhinitis in SouthEast Asia

Analysis of Allergen Components and Identification of Bioactivity of HSP70 in Pollen of Populus Deltoides

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