In this study, we investigated the possible development of threat-related attentional bias due to the work as a police officer. For this purpose, we compared four groups, differing with regard to their experience in policing and martial arts: (a) police officers with no experience in martial arts (n = 74), (b) martial artists (n = 50), (c) police officers with martial arts experience (n = 33), and (d) a control group (n = 48). Participants completed the Dot Probe Task, the Emotional Stroop Task, and the State-Trait Anxiety Inventory. The results on the Dot Probe and the Emotional Stroop Task showed that the groups did not differ with regard to threat-related attentional biases. However, the control group showed higher measures of trait anxiety compared with the other groups. The results are discussed in the light of police use of force and martial arts training, as well as with regard to optimizing psychological measures to capture functional threat-related attentional bias.