Polarized light‐scattering profile—advanced characterization of nonspherical particles with scanning flow cytometry

Strokotov, Dmitry I.; Moskalensky, Alexander E.; Nekrasov, Vyacheslav M.; Maltsev, Valeri P.

doi:10.1002/cyto.a.21074

Cited by 42 publications

(31 citation statements)

References 37 publications

(48 reference statements)

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“…Technical features of the SFC and the operational function of the optical cell were previously described in detail elsewhere (22,23). The SFC was fabricated by Cytonova LLC.…”

Section: Flow Cytometermentioning

confidence: 99%

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Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

et al. 2017

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Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above-mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle-resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label-free, enhanced morphological analysis of individual intact RBCs with compact single-detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape. © 2017 International Society for Advancement of Cytometry.

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“…Technical features of the SFC and the operational function of the optical cell were previously described in detail elsewhere (22,23). The SFC was fabricated by Cytonova LLC.…”

Section: Flow Cytometermentioning

confidence: 99%

“…A promising approach for complete and rapid characterization of RBCs is the scanning flow cytometry (SFC), based on measuring of a larger amount of light-scattering information from each cell in a flow (22,23). Specifically, light scattering profile (LSP) is measured in a wide range of scattering angles.…”

Section: Introductionmentioning

confidence: 99%

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

et al. 2017

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“…Orlova et al (2008) even validate their computed scattering results by comparisons to measurements performed with a scanning flow cytometer. Indeed, several approaches in addition to flow cytometers (Jacobs et al, 2009;Strokotov et al, 2011) have been taken towards measuring scattering by individual particles: for example, air flow systems measuring two-dimensional scattering patterns (Kaye et al, 2007) and digital holographic imaging (Berg and Videen, 2011). This paper is structured as follows: relevant light scattering theory is presented in Sect.…”

Section: H Lindqvist Et Al: Scattering By Realistic Inhomogeneous mentioning

confidence: 99%

Single scattering by realistic, inhomogeneous mineral dust particles with stereogrammetric shapes

et al. 2014

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Abstract. Light scattering by single, inhomogeneous mineral dust particles was simulated based on shapes and compositions derived directly from measurements of real dust particles instead of using a mathematical shape model. We demonstrate the use of the stereogrammetric shape retrieval method in the context of single-scattering modelling of mineral dust for four different dust types -all of them inhomogeneous -ranging from compact, equidimensional shapes to very elongated and aggregate shapes. The three-dimensional particle shapes were derived from stereo pairs of scanningelectron microscope images, and inhomogeneous composition was determined by mineralogical interpretation of localized elemental information based on energy-dispersive spectroscopy. Scattering computations were performed for particles of equal-volume diameters, from 0.08 µm up to 2.8 µm at 550 nm wavelength, using the discrete-dipole approximation. Particle-to-particle variation in scattering by mineral dust was found to be quite considerable and was not well reproduced by simplified shapes of homogeneous spheres, spheroids, or Gaussian random spheres. Effective-medium approximation results revealed that particle inhomogeneity should be accounted for even for small amounts of absorbing media (here up to 2 % of the volume), especially when considering scattering by inhomogeneous particles at size parameters 3 < x < 8. When integrated over a log-normal size distribution, the linear depolarization ratio and singlescattering albedo were also found to be sensitive to inhomogeneity. The methodology applied is work-intensive and the light-scattering method used quite limited in terms of size parameter coverage. It would therefore be desirable to find a sufficiently accurate but simpler approach with fewer limitations for single-scattering modelling of dust. For validation of such a method, the approach presented here could be used for producing reference data when applied to a suitable set of target particles.

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“…We used a scanning flow cytometer (SFC), described in detail elsewhere (37)(38)(39)(40)(41). The SFC was fabricated by Cytonova LLC (Novosibirsk, Russia).…”

Section: Instrumentationmentioning

confidence: 99%

Proposed Dynamics of CDB3 Activation in Human Erythrocytes by Nifedipine Studied with Scanning Flow Cytometry

et al. 2019

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Nifedipine is calcium channels and pumps blocker widely used in medicine. However, mechanisms of nifedipine action in blood are not clear. In particular, the influence of nifedipine on erythrocytes is far from completely understood. In this work, applying scanning flow cytometry, we observed experimentally for the first time the dynamics behind a significant increase of HCO 3 − /Cl − transmembrane exchange rate of CDB3 (main anion exchanger, AE1, Band 3, SLC4A1) of human erythrocytes in the presence of nifedipine in blood. It was found that the rate of CDB3 activation is not limited by the rate of nifedipine binding and/or Ca 2+ transport. In order to explain the experimental data, we suggested a kinetic model assuming that the rate of CDB3 activation is limited by the dynamics of the balance between two intracellular processes (1) the activation of CDB3 limited by its interaction with intracellular Ca 2+ , and (2) the spontaneous deactivation of CDB3. Thus the use of scanning flow cytometry allowed to clarify quantitatively the molecular kinetic mechanism of nifedipine action on human erythrocytes. In particular, the efficiency (~30) and rates of activation (~0.3 min −1 ) and deactivation (~10 −3 min −1 ) of CDB3 in human erythrocytes was evaluated for two donors.Nifedipine is widely used in medicine under the name of Adalat and other drugs. One common usage is the treatment of cardiovascular pathology such as atherosclerosis, coronary heart disease, hypertension, cerebral ischemia, and others (1). Other uses include painful spasms of the esophagus (2,3), Raynaud's phenomenon (4), tocolytic therapy (5). However, the effect of the treatment by nifedipine is not always positive (6). In particular, it is not clear (7) the influence of nifedipine on the rate of HCO 3− /Cl − transmembrane CDB3 protein (AE1, Band 3), which plays an important role in bicarbonate metabolism (8). Overall, a more complete understanding of nifedipine action in blood is needed.Nifedipine belongs to the subclass of dihydropyridines (DHPs), which are known as inhibitors of Ca 2+ transport across the erythrocyte membrane in both pathways: (1) inward leak through Ca 2+ entry channels (9-11), and (2) extrusion by plasma membrane Ca 2+ pumps (PMCA) (12,13). It was reported (13) that about 50% inhibition of PMCA was obtained at extracellular nifedipine concentration of 300 μM. Interestingly, the same extracellular concentration of 300 μM nifedipine was reported (14) to cause less (about 30%) inhibition of Ca 2+ entry channels of erythrocyte membrane. Stronger inhibition of PMCA than entry channels leads to the accumulation of intracellular Ca 2+ in erythrocytes in the presence of extracellular

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Polarized light‐scattering profile—advanced characterization of nonspherical particles with scanning flow cytometry

Cited by 42 publications

References 37 publications

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

Single scattering by realistic, inhomogeneous mineral dust particles with stereogrammetric shapes

Proposed Dynamics of CDB3 Activation in Human Erythrocytes by Nifedipine Studied with Scanning Flow Cytometry

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