Polar phenol detection in rat brain: Development and validation of a versatile UHPLC-MS method and application on the brain tissues of Corinthian currant (Vitis vinifera L.,var. Apyrena) fed rats

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

“…The chromatographic analysis was carried out on a Dionex UltiMate™ 3000 Rapid Separation UHPLC+ (Thermo Fisher Scientific, Bremen, Germany) coupled to an Exactive Plus™ Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany), equipped with a heated electrospray ionization probe (HESI-II; Thermo Fisher Scientific, USA) operated in negative mode. The chromatographic conditions and mass spectrometry parameters for the analysis of polar phenolics were set as previously described by Vasilakopoulou et al [ 4 ]. The total run time was 18 min.…”

“…The plasma/serum samples were initially treated as described in Section 2.4.1 . After evaporation, the residues were reconstituted in 400 μL of 0.1% ( v / v ) HCOOH and H 2 O, and were loaded onto OASIS ® HLB SPE cartridges as described by Vasilakopoulou et al [ 4 ]. Briefly, 1.5 mL of MeOH followed by the same volume of 0.1% formic acid were used to precondition the sorbent of the SPE cartridges.…”

“…One of the three commercial sub-varieties of Corinthian Currants, namely Vostizza, falls under the high-quality category and has a protected designation of origin (PDO) name. Corinthian Currants have been found to contain a plethora of polar phenolic components, including benzoic and phenyl acetic acid derivatives, hydroxy-cinnamic acids, anthocyanins and flavonoids [ 1 , 2 , 3 , 4 ]. While an increasing number of studies have shown a correlation between the consumption of polar phenolics and a reduction in chronic disease risk factors, discrepancies in the explanation of their beneficial effects have been found in terms of their bioavailability [ 5 , 6 ].…”

“…The % extraction recoveries (% R), which served as the main evaluation index, were obtained by comparing spiked versus matrix-matched plasma and serum samples in each case. Ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) was used for the detection and quantification of the studied polar phenolic moieties [ 4 ].…”

The Spectrum of Pharmacological Actions of Syringetin and Its Natural Derivatives—A Summary Review

“…The most recent literature reports indicate that being an ingredient of a novel drug Bushao Tiaozhi Capsule (BSTZC), isorhamnetin may help to regulate the lipids level in the blood, and therefore may be used in the treatment of hyperlipidemia [ 87 ]. In addition, an in vivo study on rats showed that isorhamnetin delivered as one of the ingredients of Corinthian currant (dried fruits) easily comes across the blood–brain barrier [ 88 ].…”