Pod set and Pollen Viability Studies in Yard Long Bean (Vigna unguiculata sub sp. sesquipedalis)

G, Merin E; Sarada, S.; Celine, V.A.

doi:10.24154/jhs.2019.v14i02.015

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…The rate of successful in hand pollination revealed by this study is comparable to hand pollination success of 2.3 -22.5 % in beans reported by Bhanu et al (2018). Similarly, Merin et al (2019) reported rate of successful in bean hand pollination of 23.8 %, while Vollmann et al (1992) reported rate of successful in hand pollination of beans below 18 %. The low pod setting of the hand pollinated flowers in beans is reported to be due to failure of the pollen tubes to penetrate the stigma and in some cases, fertilization occurs, but embryo abortion takes place due high temperature above 23 ˚C, genetic response of the parents and relative humidity below 45 or higher than 55 (Bhanu et al, 2018).…”

Section: Common Bean F2 Lines Seed Iron and Zinc Contentssupporting

confidence: 82%

Iron and zinc genetic biofortification of yellow common bean (phaseolus vulgaris l.) genotypes in Tanzania

Philipo

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Iron and zinc deficiencies are the global leading micronutrient deficiencies particularly in developing countries such as Tanzania. Iron deficiency in humans causes anemia, whereas zinc deficiency leads to compromised immunity, decreased growth rate, and mental retardation. This study was conducted in the Northern, Eastern, and Southern Highlands of Tanzania from 2018 to 2020 to address the iron and zinc deficiencies in humans using genetic biofortification. The approach involved screening common bean genotypes for iron and zinc contents and the factors (such as phytic acid, and ferritin) that inhibit and or enhance their availability in the human gut. The genotypes with high seed iron and zinc contents were used in the genetic biofortification of the consumers’ preferred yellow bean varieties. Field experiments (involving 99 common bean genotypes) were conducted at TARI-Selian, SUA, and TARI-Uyole to screen common bean genotypes for seed minerals and yield. The genotypes were planted following alpha lattice design in three replications each contained five blocks with 20 plots. Data were recorded for days to 75% flowering, number of pods/plant, number of seeds/pod, 100 seed weight, and seed yield. Furthermore, the contents of seed iron, zinc, phosphorus, magnesium, manganese, ferritin, phytic acid, and phytic acid to mineral molar ratios were determined. Results showed that there were highly significant (P ≤ 0.001) effects between bean genotypes, environments, and genotype by environment interaction on seed yield, yield related traits, seed iron, and zinc contents. The highest and stable bean genotypes for seed iron and zinc contents and seed yield were identified. High phosphorus, magnesium, manganese, and low phytic acid and phytic acid to mineral molar ratio bean genotypes were also identified. Furthermore, the study developed F2 bean crosses with a 12.5 - 146.4 % increase in seed iron content and a 1.0 - 53.1 % increase in zinc content. Seventeen of the developed F2 crosses had a high seed iron content ≥ 70 mg/kg and seed zinc content ≥ 30 mg/kg, these include BF01, BF04. BF05, BF06, BF08, BF10, BF13, BF16, BF22, BF24, BF25, BF27, BF29, BF31, BF32, BF33, and BF35.

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Section: Common Bean F2 Lines Seed Iron and Zinc Contentssupporting

confidence: 82%

Iron and zinc genetic biofortification of yellow common bean (phaseolus vulgaris l.) genotypes in Tanzania

Philipo

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“…The overall rate is 49.82% lower compared to Yard Long Bean (Vigna unguiculata subsp. sesquipedalis) (46).…”

Section: Pollen Viability Assaymentioning

confidence: 99%

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

et al. 2021

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Pollen viability, germination and compatibility are essential in determining the success of pollination and seed setting of high-valued crops. Rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) is an underutilized and unexplored indigenous legume with high potential for commercial production. In this study, pollen quality, viability, germination rate and incompatibility among selected six rice bean (V. umbellata) accessions from Barili, Cebu, Philippines were evaluated to determine the barriers and effective pollination habit for increased productivity while retaining the important traits, including high tolerance in poor soils, superior climatic resilience and resistance to pest and diseases. Results of acetocarmine calorimetric assay showed that rice beans’ (V. umbellata) pollens are highly viable, with accessions VU 004 (56.33 ± 4.91%) and VU 007 (54.34 ± 4.53%) having the optimum viability rate. Brewbaker and Kwack medium treated with 0.2 g.l-1 and 0.3 g.l-1 boric acid (H3BO4) enhanced the germination rate in vitro (11.56 ± 5.53% and 9.47 ± 6.50% respectively). Bud (14.96 ± 1.53%) and post-anthesis pollens (10.28 ± 0.94%) have optimum germination rate in 0.2 g.l-1 boric acid media, while anthesis pollens are suitable in media supplemented with sucrose and boric acid alone (12.20 ± 1.50%) and with 0.1 g.l-1 myo-inositol supplementation (8.49 ± 1.86%). Pollination test revealed that rice bean accessions have high self-compatibility (50.76 + 3.45%) and low cross-compatibility (26.57 + 2.49%). The findings provide an important background in understanding the pollen quality and intraspecific interaction among indigenous rice bean (V. umbellata) accessions in Barili, Cebu to improve production and hybridization.

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Intraspecific-cross compatibility in cowpea (Vigna unguiculata (L.) Walp.)

Amusa

Ogunkanmi

Adetumbi

et al. 2021

Journal of Crop Improvement

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Pod set and Pollen Viability Studies in Yard Long Bean (Vigna unguiculata sub sp. sesquipedalis)

Cited by 3 publications

References 5 publications

Iron and zinc genetic biofortification of yellow common bean (phaseolus vulgaris l.) genotypes in Tanzania

Iron and zinc genetic biofortification of yellow common bean (phaseolus vulgaris l.) genotypes in Tanzania

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

Intraspecific-cross compatibility in cowpea (Vigna unguiculata (L.) Walp.)

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