PLS Regression in the Spectrophotometric Data for the Simultaneous Determination of Levodopa and Carbidopa in Pharmaceutical Preparations by Using an Enzymatic Stopped‐Flow FIA Technique

Abstract

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Kinetic approach for the enzymatic determination of levodopa and carbidopa assisted by multivariate curve resolution-alternating least squares

“…The region comprised within 190 and 288 nm shows the absorbance of some excipients present in the samples [16]. Thus, in a first approach, the wavelengths from 288 to 600 nm were selected.…”

“…In spite of the structural similarity between the analytes, different products are obtained from the enzymatic reaction [16]. Fig.…”

“…Most of the studies are aided by chemometrics tools. Thus, some studies using parallel factor analysis (PARAFAC) [21,22], multivariate curve resolution-alternating least squares (MCR-ALS) [23,24], partial least squares (PLS) [16,25,26], three way partial least squares (N-PLS) [27,28] and artificial neural networks (ANN) [29,30] were reported.…”

“…These are commonly carried out by potentiometry [3], voltammetry [4,5], high performance liquid chromatography (HPLC) [6][7][8], capillary electrophoresis (CE) [9,10], NMR 1 H [11], fluorescence [12], synchronous fluorescence [13] and UV-vis spectrophotometry [14][15][16][17].…”

Simultaneous estimation of levodopa and carbidopa by RP‐HPLC using a fluorescence detector: its application to a pharmaceutical dosage form

“…A kinetic H‐point standard addition method has been reported for the simultaneous determination of LD and CD without any interference . Methods using HPLC , nuclear magnetic resonance spectroscopy , capillary electrophoresis and enzymatic stopped‐flow injection have also been reported. Some of these can also be used in the estimation of LD and CD with other catecholamines (noradrenaline, dopamine, l ‐tyrosine, etc.)…”