Plectranthus barbatus Andrews as anti-Helicobacter pylori agent with activity against adenocarcinoma gastric cells

Borges, Augusto Santos; Minozzo, Bruno Rodrigo; Santos, Heloá; Ardisson, Juliana Santa; Rodrigues, Rossana Lott; Romão, Wanderson; Borges, Warley de Souza; Gonçalves, Rita de Cássia Ribeiro; Beltrame, Flávio Luís; Kitagawa, Rodrigo Rezende

doi:10.1016/j.indcrop.2020.112207

Cited by 15 publications

(13 citation statements)

References 72 publications

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“…Although they correlated these results to the presence of forskolin and coleusin factor, they did not isolate them, but mentioned that these molecules showed cytotoxicity to murine melanoma cells (Agarwal and Parks Junior, 1983) and osteosarcoma (Geng et al, 2014). In addition, Borges et al (2020) identified an antineoplastic activity (IC50 = 33.89 ± 0.32 µg.mL -1 ) of ethyl acetate fraction (EAF) of P. barbatus leaves, but the identified constituents of EAF were plectrin, hydrolyzed abieatane, barbatusin, 3β-hydroxy-3-deoxibarbatusin, cyclobutatusin, luteolin, rosmarinic acid and coleoside B which we did not detect in our extracts.…”

Section: Resultsmentioning

confidence: 99%

“…Other phytochemicals such as the phenolic derivatives nepetoidins A and B (Grayer et al, 2003) and rosmarinic acid (Falé et al, 2011) were also found in P. barbatus composition (Silva et al, 2016). Phenolic plant-derived compounds comprise the composition of several classes of molecules such as simple phenolics, phenolic acids, coumarins, flavonoids, hydrolysable and condensed tannins, stilbenes, lignans and lignins (Silva et al, 2019) and, some of these have already been identified in P. barbatus extracts (Borges et al, 2020;Ezeonwumelu et al, 2019;Rodrigues et al, 2016).…”

Section: Introductionmentioning

confidence: 96%

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Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

et al. 2022

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Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

et al. 2022

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“…There was variability in the study regions for the selected manuscripts, with 25% of the papers coming from Asia ( Moon et al, 2011 ; Asha et al, 2013 ; Manayi et al, 2013 ; Wang et al, 2014 ; Park et al, 2016 ; Ibrahim et al, 2018 ; Kim et al, 2018 ; Lu et al, 2018 ; Saravanakumar et al, 2019 ; Ma X. et al, 2020 ; Das et al, 2020 ), 56.82% from America, of which 40.91% were from South America ( Adzu et al, 2015 ; Almeida et al, 2019 ; Arunachalam et al, 2019 ; Borges et al, 2020 ; da Silva Junior et al, 2016 ; de Cássia dos Santos et al, 2019 ; Garro et al, 2015 ; Klein-Júnior et al, 2013 ; Marcial et al, 2014 ; Mazzolin et al, 2010 ; Minozzo et al, 2016 ; Miranda et al, 2015 ; Mota Da Silva et al, 2015 ; Oliveira et al, 2015 ; Pastene et al, 2014 ; Ribeiro et al, 2013 ; Santos et al, 2012 ; Spósito et al, 2019 ) and 15.91% from North America ( Ankolekar et al, 2011 ; Brown and Jiang, 2013 ; Escobedo Hinojosa et al, 2014 ; Palacios-Espinosa et al, 2014 ; Espinosa-Rivero et al, 2015 ; Egas et al, 2018 ; Gomez-Chang et al, 2018 ), 9.09% of the articles originated from the Africa ( Okeleye et al, 2011 ; Lawal et al, 2014 ; Hamad et al, 2015 ; Fahmy et al, 2020 ), and 9,09% from the Europe ( Bisignano et al, 2013 ; Cardoso et al, 2018 ; Zengin et al, 2018 ; Cesa et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Flavonoids-Rich Plant Extracts Against Helicobacter pylori Infection as Prevention to Gastric Cancer

et al. 2022

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Gastric cancer is the fifth most common and fourth type to cause the highest mortality rates worldwide. The leading cause is related to Helicobacter pylori (H. pylori) infection. Unfortunately, current treatments have low success rates, highlighting the need for alternative treatments against carcinogenic agents, specifically H. pylori. Noteworthy, natural origin products contain pharmacologically active metabolites such as flavonoids, with potential antimicrobial applications.Objective: This article overviews flavonoid-rich extracts’ biological and pharmacological activities. It focuses on using these substances against Helicobacter pylori infection to prevent gastric cancer. For this, PubMed and Science Direct databases were searched for studies that reported the activity of flavonoids against H. pylori, published within a 10-year time frame (2010 to August 2020). It resulted in 1,773 publications, of which 44 were selected according to the search criteria. The plant family primarily found in publications was Fabaceae (9.61%). Among the flavonoids identified after extraction, the most prevalent were quercetin (19.61%), catechin (13.72), epicatechin (11.76), and rutin (11.76). The potential mechanisms associated with anti-H. pylori activity to the extracts were: inhibition of urease, damage to genetic material, inhibition of protein synthesis, and adhesion of the microorganism to host cells.Conclusion: Plant extracts rich in flavonoids with anti-H. pylori potential proved to be a promising alternative therapy source, reinforcing the relevance of studies with natural products.

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“…The determination of the minimum inhibitory concentration (MIC) of EO against H. pylori was evaluated using the broth microdilution assays in 96-well plates, according to the Standards for Antimicrobial Susceptibility Testing protocol [ 69 ], with adaptations [ 70 ]. The EO was diluted with the BHI broth supplemented with 10% foetal bovine serum and dimethylsulfoxide 2% (DMSO, Sigma) at 47 °C to prepare the concentrations at 6 μL/mL–25 μL/mL (0.6–2.5% v / v ).…”

Section: Methodsmentioning

confidence: 99%

“…A total of 100 µL aliquots of overnight bacterial suspension (approximately 10 6 CFU/mL; 1:20 0.5 McFarland Standard) [ 70 ] was added to each well of a 96-well plate. The cultures were incubated with 100 µL aliquots of EO for 72 h at 37 °C under microaerobic conditions (CampyGen) with shaking (120 rpm).…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition and In Vitro Anti-Helicobacter pylori Activity of Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae) Essential Oil

Neves

Mello

Smith

et al. 2022

Plants

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Helicobacter pylori is the most common cause of gastritis and peptic ulcers, and the number of resistant strains to multiple conventional antimicrobial agents has been increasing in different parts of the world. Several studies have shown that some essential oils (EO) have bioactive compounds, which can be attributed to antimicrobial activity. Therefore, EOs have been proposed as a natural alternative to antibiotics, or for use in combination with conventional treatment for H. pylori infection. Campomanesia lineatifolia is an edible species found in the Brazilian forests, and their leaves are traditionally used for the treatment of gastrointestinal disorders. Anti-inflammatory, gastroprotective, and antioxidant properties are attributed to C. lineatifolia leaf extracts; however, studies related to the chemical constituents of the essential oil and anti-H. pylori activity is not described. This work aims to identify the chemical composition of the EO from C. lineatifolia leaves and evaluate the anti-H. pylori activity. The EO was obtained by hydrodistillation from C. lineatifolia leaves and characterized by gas chromatography–mass spectrometry analyses. To assess the in vitro anti-H. pylori activity of the C. lineatifolia leaf’s EO (6 μL/mL–25 μL/mL), we performed broth microdilution assays by using type cultures (ATCC 49503, NCTC 11638, both clarithromycin-sensitive) and clinical isolate strains (SSR359, clarithromycin-sensitive, and SSR366, clarithromycin-resistant). A total of eight new compounds were identified from the EO (3-hexen-1-ol (46.15%), α-cadinol (20.35%), 1,1-diethoxyethane (13.08%), 2,3-dicyano-7,7-dimethyl-5,6-benzonorbornadiene (10.78%), aromadendrene 2 (3.0%), [3-S-(3α, 3aα, 6α, 8aα)]-4,5,6,7,8,8a-hexahydro-3,7,7-trimethyl-8-methylene-3H-3a,6-methanoazulene (2.99%), α-bisabolol (0.94%), and β-curcumene (0.8%)), corresponding to 98.09% of the total oil composition. The EO inhibited the growth of all H. pylori strains tested (MIC 6 μL/mL). To our knowledge, the current study investigates the relation between the chemical composition and the anti-H. pylori activity of the C. lineatifolia EO for the first time. Our findings show the potential use of the C. lineatifolia leaf EO against sensitive and resistant clarithromycin H. pylori strains and suggest that this antimicrobial activity could be related to its ethnopharmacological use.

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Plectranthus barbatus Andrews as anti-Helicobacter pylori agent with activity against adenocarcinoma gastric cells

Cited by 15 publications

References 72 publications

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

Phytochemical characterization and biological activities of Plectranthus barbatus Andrews

Flavonoids-Rich Plant Extracts Against Helicobacter pylori Infection as Prevention to Gastric Cancer

Chemical Composition and In Vitro Anti-Helicobacter pylori Activity of Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae) Essential Oil

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