Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis

Akeda, Koji; An, Howard S.; Okuma, M; Attawia, Mohamed; Miyamoto, Kei; Thonar, Eugene J-M.A.; Lenz, Mary Ellen; Sah, Robert L.; Masuda, Koichi

doi:10.1016/j.joca.2006.05.008

Cited by 359 publications

(272 citation statements)

References 53 publications

(56 reference statements)

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“…Our explanation was already supported by Gulotta et al, who suggested that the majority of patients who undergo this surgery experience immediate chondrocyte necrosis after surgery, followed by a reincorporation of their grafts [11]. In this process, PRP may have a reinforcement on chondral regeneration and proliferation of chondrocytes as a biologic inducer in the short term [16,26].…”

Section: Discussionmentioning

confidence: 51%

“…PRP contains growth factors such as platelet-derived growth factor, TGF-B, FGF, insulin growth factor, vascular endothelial growth factor, and epithelial cell growth factor [15]. In fact, platelet concentrate has been shown to promote bone healing, chondrocyte proliferation, and cartilage formation in orthopaedics [15][16][17]. Conversely, in a very recent review, the authors suggested that there is limited evidence to support the use of PRP in the management of chondral or osteochondral defects, whether alone or as an adjunct to surgical treatment [18].…”

Section: Introductionmentioning

confidence: 99%

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The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

Altan

Aydın

Erkoçak

et al. 2014

International Orthopaedics (SICOT)

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Purpose This study investigated the efficacy of platelet-rich plasma (PRP) on articular surfaces on which the mosaicplasty technique was performed. Our hypothesis was that PRP can accelerate the osseointegration process and enhance the quality of articular integrity after the mosaicplasty procedure. Methods Standard defects were created in the femoral groove of both patellofemoral joints of 12 New Zealand rabbits. PRP solution was placed inside the defect before fixation of the osteochondral autografts and injected inside the involved joint after capsular closure of the tested knees. The contralateral knees served as the control sides. The animals were euthanized three or six weeks after mosaicplasty, and both limbs were assessed according to Pineda's histological grading scale. Significance level was set at p≤0.05 a priori, and the Mann-Whitney U test was used for statistical analysis. Results Histologic findings at the interface between the transferred autograft and the original cartilage revealed better integration of the adjacent surfaces in the mosaicplasty with PRP group three weeks after the procedure; the difference was significant (p<0.05). However, no significant difference in the transition zone was observed between the groups six weeks after the experiment (p=0.59). Conclusions Our animal model showed that adjunctive use of PRP produced a better healing response and resulted in superior histological scores after three weeks compared with the mosaicplasty-only procedure. Interpretation of our results is important in terms of rapid return to previous activity levels. Thus, application of PRP can represent a valid therapeutic option for improving the efficacy of mosaicplasty by stimulating the local healing response.

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Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

Altan

Aydın

Erkoçak

et al. 2014

International Orthopaedics (SICOT)

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“…Additionally, the multitude of growth factors stored within the platelets' alpha granules are believed to improve the biological environment within which cartilage may heal (Smyth et al 2012). Several in vivo and in vitro published studies delineate the potential of PRP for improving chondrogenesis in ankle cartilage repair (Akeda et al 2006;Milano et al 2010).…”

Section: Discussionmentioning

confidence: 99%

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Ghiasi

Kalhor

Qomi

et al. 2015

Frontiers in Life Science

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This study presents a comparative assessment of adipose-derived stem cell (ADSCs) proliferation rates and their viability on five different scaffolds. Five different biomaterial scaffolds were prepared: alginate, poly lactic-co-glycolic acid, fibrin glue, inactive platelet-rich plasma, and active plateletrich plasma (APRP). Stem cells were isolated from human adipose tissue. Flow cytometry analysis was performed. Specifically, adipogenesis/osteogenesis/chondrogenesis-associated genes expression was analyzed by real-time polymerase chain reaction. These cells were seeded in the prepared scaffolds. After 14 days, the proliferation and viability of MSCs were evaluated using an MTT assay. Also, stemness genes expression was analyzed with the reverse transcriptasepolymerase chain reaction (RT-PCR) method. In addition, the DNA content assay was also performed. The obtained results showed a significant difference between cell proliferation and viability of different scaffolds. APRP and alginate were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p < .05). RT-PCR reactions demonstrated the expression of the various stemness-related markers (Nanog, Octamer4A, and Sox2) when ADSC cells were grown separately on the five different scaffolds. Our study indicates that compared with the scaffolds, APRP could be the best scaffold for support of ADSC proliferation. ARTICLE HISTORY

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“…362 The in vitro literature indicates that this biological adjunct may increase chondrocyte proliferation as well as synthetic capability, while limiting the catabolic effects of an inflammatory joint environment. 10,233,287,418 In a rabbit model, the addition of PRP showed improved healing of OCDs treated with microfracture surgery. 284 Human studies have reported variable effects of PRP injections alone as well as in addition to surgery.…”

Section: Part IV -Alternative Treatmentmentioning

confidence: 96%

“…225,275 PDGF may promote mitogenesis, angiogenesis, and chondrocyte proliferation. 10,225,275 Although, in theory, PRP may enhance the biologic activity of DBM, the combination of DBM and PRP has had contradictory results. 213,275,324 Th e purpose of the present study was to evaluate the eff ectiveness of DBM with or without PRP in the treatment of ankle OCDs in goats.…”

mentioning

confidence: 99%

Treatment of osteochondral defects of the talus

Bergen

Leeuw

Dijk

2008

Revue de Chirurgie Orthopédique et Réparatrice de l'Appareil Mo

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Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis

Abstract: PRP isolated from autologous blood may be useful as a source of anabolic growth factors for stimulating chondrocytes to engineer cartilage tissue.

Cited by 359 publications

References 53 publications

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

The effect of platelet-rich plasma on osteochondral defects treated with mosaicplasty

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Treatment of osteochondral defects of the talus

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