Platelet-rich fibrin (PRF) membranes accelerate open wound healing better than amniotic membranes: A histological study on the proliferation phase

Sari, Rezmelia; Larasati, Grace Sekar; Kuncorowati, Nurul Ghoutsiyah; Syaify, Ahmad

doi:10.1016/j.wndm.2020.100190

Cited by 8 publications

(10 citation statements)

References 26 publications

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“…The treatment group had a higher collagen density than the control group during both healing periods. These results were in line with a study conducted by Kiani et al 13 , in which collagen was found to be visible in the wound site on the third day to 2 weeks after injury. On the seventh day after the injury, more natural collagen fibers and fibroblast cells promotion were found.…”

Section: Resultssupporting

confidence: 93%

“…On the fourteenth day after the injury, collagen fibers will reach their stability and become a more organised arrangement on the wound site. 13 Sari et al 14 investigated the differences in the wound healing process during the proliferation phase after applying PRF and amniotic membranes. The results showed that fibroblasts, angiogenesis, collagen density, and epithelial thickness were higher in the PRF membranes group than in the amniotic membranes or the control group.…”

Section: Resultsmentioning

confidence: 99%

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<strong>Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits</strong>

Nasution

Dewanti

2022

Padjadjaran J Dent

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Introduction: Due to the high prevalence of periodontal diseases that increase the possibility of tooth loss, platelet-rich fibrin (PRF) as a regenerative treatment has been proposed. PRF is a potential second-generation platelet concentrate and autologous healing biomaterial which contain growth factors that accelerate wound healing mechanisms. This study aimed to evaluate the efficacy of platelet-rich fibrin application to improve gingival connective tissue collagen density in periodontitis-induced rabbits. Methods: An experimental study with a post-test only control group design approach was conducted on 20 samples calculated using the Federer formula. Periodontitis was induced in ten rabbits with silk ligatures tied around the cervical region of central incisors teeth bilaterally for 14 days. After periodontitis occurred, phase I treatment was performed and followed by curettage. PRF was placed in the lower region as a treatment group using a split-mouth design, whereas a control group without PRF was in the upper jaw region. Subjects were euthanised after the 7th and 14th days of healing. Histopathological analysis was performed, and collagen density was quantified using ImageJ software. Statistical analysis was carried out using paired-T and two-way ANOVA tests. Results: There was a significant difference (p=0.001; p<0.05) in gingival connective tissue collagen density between treatment and control groups on the 7th and 14th days of observation. There was a significant increase in gingival connective tissue collagen density in the treatment group. Conclusion: Platelet-rich fibrin could improve gingival connective tissue collagen density in periodontitis-induced rabbits.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

<strong>Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits</strong>

Nasution

Dewanti

2022

Padjadjaran J Dent

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“…This procedure was performed in the dermis region, to obtain representative results, the papillary and reticular layers were analyzed. 2 Cell counting was performed using ImageJ ® software using the "Cell counter" plugin.…”

Section: Macroscopic and Histological Evaluation Of Lesionsmentioning

confidence: 99%

“…1 In this sense, these injuries are often covered by wound dressings to prevent the accumulation of exudate, as well as to improve wound healing and prevent infection of the injured area by pathogens. 2 The wound healing process is dynamic and involves the steps of hemostasis, inflammation, proliferation, and remodeling. 3 In the United States of America, $8.2 million Medicare beneficiaries had some type of wound or infection in 2014.…”

Section: Introductionmentioning

confidence: 99%

Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca²⁺ cross‐linking for wound healing applications

et al. 2022

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This work aimed the development and evaluation of the wound healing activity of films based on sodium alginate, polyvinyl alcohol (PVA) and Ca2+ loaded with Agaricus blazei Murill hydroalcoholic extract (AbE). Firstly, AbE was prepared using a previously standardized methodology. The films were prepared by casting technique and cross‐linked with Ca2+ using CaCl2 as cross‐linking agent. The physicochemical, morphological and water vapor barrier properties of the films were analyzed and the pre‐clinical efficacy was investigated against the cutaneous wound model in mice. The films showed barrier properties to water vapor promising for wound healing. AbE showed physical and chemical interactions between both polymers, noticed by Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, and thermal analysis. The delivery of AbE in alginate/PVA films enhanced the antioxidant and wound healing properties of these polymers. Consequently, a reduction of malondialdehyde levels was observed, as well as an increase of the epidermis/dermis thickness and enhancement in collagen I deposition. Thus, these formulations are promising biomaterials for wound care and tissue repairing.

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“…Head-to-head clinicohistological studies have placed PRF superior to other biological membranes in terms of reduction of wound diameter and depth, including amniotic membrane, topical chlorhexidine gel, normal saline, topical metronidazole and conventional dressings including zinc oxide (Unna's paste) for various etiologies. [ 41 42 43 44 45 ] It is considered at par with other modalities like hyperbaric oxygen therapy, split skin and full thickness skin grafting, and vacuum-assisted negative pressure wound dressing. [ 46 ] The utility of PRF has extended into the field of wound healing by secondary intention via hemostasis, cellular chemotaxis and proliferation, angiogenesis, and extracellular matrix induction.…”

Section: Use Of Prf In Dermatologymentioning

confidence: 99%

Platelet-rich fibrin, preparation and use in dermatology

Dashore¹,

Chouhan²,

Nanda³

et al. 2021

Indian Dermatol Online J

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The goal of these recommendations is to provide a framework to practitioners for implementing useful, evidence-based recommendations for the preparation of platelet-rich fibrin (PRF) and its use in various dermatological indications. The Indian Association of Dermatologists, Venereologists and Leprologists (IADVL) assigned the task of preparing these recommendations to its taskforce on platelet-rich plasma. A comprehensive literature search was done in the English language on the PRF across multiple databases. The grade of evidence and strength of recommendation was evaluated on the GRADE framework (Grading of Recommendation, Assessment, Development and Evaluation). A draft of clinical recommendations was developed on the best available evidence which was also scrutinized and critically evaluated by the IADVL Academy of Dermatology. Based on the inputs received, this final consensus statement was prepared. A total of 40 articles (meta-analyses, prospective and retrospective studies, reviews [including chapters in books] and case series) were critically evaluated and the evidence thus gathered was used in the preparation of these recommendations. This expert group recommends use of A-PRF+ protocol, that is (200 g for 8 min) for preparation of solid PRF and C-PRF protocol (700 g for 8 min) for liquid PRF. Swing out bucket model of centrifuge or the horizontal centrifuge is recommended for preparation of both PRF, and liquid PRF. Centrifugation must begin within 90–120 s of drawing of blood. PRF can be used in various indications for skin rejuvenation and nonhealing ulcers as either monotherapy or in combination with other therapies.

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Platelet-rich fibrin (PRF) membranes accelerate open wound healing better than amniotic membranes: A histological study on the proliferation phase

Cited by 8 publications

References 26 publications

<strong>Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits</strong>

<strong>Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits</strong>

Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca²⁺ cross‐linking for wound healing applications

Platelet-rich fibrin, preparation and use in dermatology

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Platelet-rich fibrin (PRF) membranes accelerate open wound healing better than amniotic membranes: A histological study on the proliferation phase

Cited by 8 publications

References 26 publications

<strong>Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits</strong>

<strong>Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits</strong>

Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca2+ cross‐linking for wound healing applications

Platelet-rich fibrin, preparation and use in dermatology

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Product

Resources

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Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca²⁺ cross‐linking for wound healing applications