Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca²⁺ cross‐linking for wound healing applications

Abstract

This work aimed the development and evaluation of the wound healing activity of films based on sodium alginate, polyvinyl alcohol (PVA) and Ca2+ loaded with Agaricus blazei Murill hydroalcoholic extract (AbE). Firstly, AbE was prepared using a previously standardized methodology. The films were prepared by casting technique and cross‐linked with Ca2+ using CaCl2 as cross‐linking agent. The physicochemical, morphological and water vapor barrier properties of the films were analyzed and the pre‐clinical efficacy was investigated against the cutaneous wound model in mice. The films showed barrier properties to water vapor promising for wound healing. AbE showed physical and chemical interactions between both polymers, noticed by Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, and thermal analysis. The delivery of AbE in alginate/PVA films enhanced the antioxidant and wound healing properties of these polymers. Consequently, a reduction of malondialdehyde levels was observed, as well as an increase of the epidermis/dermis thickness and enhancement in collagen I deposition. Thus, these formulations are promising biomaterials for wound care and tissue repairing.

Polysacharide of Agaricus blazei gel mitigates bone necrosis in model of the jaws related to bisphosphonate via Wnt signaling

“…Finally, a process of intense agitation was successfully carried out, followed by cooling at 5 °C for 3 days to obtain a homogeneous phase of polymer, solvent and drug. The gels were prepared after investigating the physical properties, specificity, viscosity, kinetics and stability of the drug, in the following concentrations: 1.0% Agaricus blazei -glucan polysaccharides Gel (1.0% w/v PAb) 17 .…”