We are writing to request a better explanation of the method of venipuncture to produce platelet-rich fibrin (PRF) in rabbits in the article recently published by Weng et al. 2 Additionally, the authors of the present letter would like to highlight the use of rabbits for this captivating study not only because of the use of the surgical model but also because rabbits are considered one of the best species for examining platelets. 1 Different sites can be used for venipuncture in rabbits, such as the ear, jugular vein, and heart. Each site has its advantages and disadvantages when it comes to producing PRF.Venipuncture in the ear is the most common method in rabbits, and it is less invasive and easily accessible. However, the amount of blood that can be collected is limited and does not offer adequate blood flow.We have concerns about the PRF produced in the paper: (1) tubes containing silica were used to activate clot formation in a tube because of the low blood flow, and (2) an adequate analysis of the release of cytokines from the PRF was not provided.In their article, Weng et al. 2 should clarify these aspects because they go beyond an experimental surgical assessment. Moreover, as a translational study, a comparison with human beings in the Discussion section would help readers understand the relevance of the study's findings.In future studies, the authors should consider using the jugular vein for venipuncture in rabbits, as it allows for the collection of a faster and more significant amount of blood than the marginal ear vein, making it less stressful for the rabbit than a heart puncture. Although locating the jugular vein in rabbits can be difficult, and the procedure can still cause discomfort, it is essential to consider the circumstances of each case and ensure the best possible care for each rabbit.