Platelet rich fibrin - a novel acumen into regenerative endodontic therapy

Hotwani, Kavita; Sharma, Krishna Kumar

doi:10.5395/rde.2014.39.1.1

Cited by 53 publications

(36 citation statements)

References 41 publications

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“…It is formulated to produce a homogeneously-moisturized thick membrane and an exudate rich in platelets, leukocytes, vitronectin, and fibronectin expressed by fibrin clots [5] .…”

Section: Preparationmentioning

confidence: 99%

Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®

Crisci¹,

Lombardi²,

Serra³

et al. 2017

JUMD

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Aim:The hypothesis is that the production of leukocyte and platelet-rich fibrin (L-PRF) is easy in horses without modifying human protocol, thus allowing better standardization of human protocol. In this study, the aim is to standardize the production of L-PRF in horses to direct it to human production. Methods: The authors took 9 mL of blood from the jugular vein of 6 horses to produce L-PRF membranes at a temperature > 21 °C by measuring the size of the membranes (height, length, thickness, weight, surface) and clot. Therefore we analyzed their microbiological characteristics. Results: The production of leukocyte and platelet-rich fibrin (L-PRF) is easy in horses without modifying human protocol, thus allowing better standardization of human protocol. The parameters found in the horse are clearly similar to the parameters found in humans. In optical microscopy, most of the cellular bodies were found and concentrated in the proximal portion of each membrane, with the last 1/4 observed in the center; the distal part only had residual traces of cellular bodies. The L-PRF is composed constantly when the production process below described is strictly respected. The success of L-PRF depends entirely on blood collection, on the quick transfer to a centrifuge (within 1 min) and on centrifugation and squeezing temperatures (between 21 and 30 °C) of the clot. Conclusion:The experiments on horses for the standardization of L-PRF production will improve our understanding about wound healing, particularly in the regenerative therapy of chronic skin lesions in humans. The data collected show that the best preparation method is the 2 min compression of the clot after 0 min of blood sampling, using the 9 mL vacutainer system and not a 9 mL syringe. Key words:Autologous, buffy coat, growth factor level, platelet-rich fibrin, thrombocyte concentrate ABSTRACTArticle history:

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“…It is formulated to produce a homogeneously-moisturized thick membrane and an exudate rich in platelets, leukocytes, vitronectin, and fibronectin expressed by fibrin clots [5] .…”

Section: Preparationmentioning

confidence: 99%

Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®

Crisci¹,

Lombardi²,

Serra³

et al. 2017

JUMD

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“…They are a natural source of growth factors such as transforming growth factor, vascular endothelial growth factor, and platelet-derived growth factor 28. The use of platelet concentrates, particularly PRP and platelet rich fibrin (PRF) is a current treatment approach in oral and maxillofacial surgery, particularly in implant dentistry 29. The main purpose of the use of PRP is to increase the speed of healing of the soft and hard tissue.…”

Section: Discussionmentioning

confidence: 99%

The use of platelet rich plasma in the treatment of immature tooth with periapical lesion: a case report

et al. 2014

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This study describes the treatment of an immature permanent tooth with periapical lesion which was treated with regenerative approach using platelet rich plasma (PRP). The root canal of immature human permanent tooth with periapical lesion was gently debrided of necrotic tissue and disinfected with 2.5% NaOCl, and then medicated with triple antibiotic paste comprised of ciprofloxacin, metronidazole, and tetracycline. When the tooth was asymptomatic, PRP and mineral trioxide aggregate (MTA) were placed into the root canal. Six months after PRP treatment, radiographical examination revealed resolution of the radiolucency and progressive thickening of the root wall and apical closure. Our findings suggest that PRP can be used for the treatment of immature permanent teeth with periapical lesion, as part of a regenerative endodontic treatment procedure.

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“…[22] The procedure consists of drawing blood which is collected into test tubes without an anticoagulant and is centrifuged instantaneously. A tabletop centrifuge can be used for 10 min at 3000 rpm or for 12 min at 2700 rpm.…”

Section: Scaffoldsmentioning

confidence: 99%

Scaffolds in regenerative endodontics: A review

Gathani

Raghavendra

2016

Dent Res J

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Root canal therapy has enabled us to save numerous teeth over the years. The most desired outcome of endodontic treatment would be when diseased or nonvital pulp is replaced with healthy pulp tissue that would revitalize the teeth through regenerative endodontics. ‘A search was conducted using the Pubmed and MEDLINE databases for articles with the criteria ‘Platelet rich plasma’, ‘Platelet rich fibrin’, ‘Stem cells’, ‘Natural and artificial scaffolds’ from 1982–2015’. Tissues are organized as three-dimensional structures, and appropriate scaffolding is necessary to provide a spatially correct position of cell location and regulate differentiation, proliferation, or metabolism of the stem cells. Extracellular matrix molecules control the differentiation of stem cells, and an appropriate scaffold might selectively bind and localize cells, contain growth factors, and undergo biodegradation over time. Different scaffolds facilitate the regeneration of different tissues. To ensure a successful regenerative procedure, it is essential to have a thorough and precise knowledge about the suitable scaffold for the required tissue. This article gives a review on the different scaffolds providing an insight into the new developmental approaches on the horizon.

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Platelet rich fibrin - a novel acumen into regenerative endodontic therapy

Cited by 53 publications

References 41 publications

Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®

Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®

The use of platelet rich plasma in the treatment of immature tooth with periapical lesion: a case report

Scaffolds in regenerative endodontics: A review

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