Platelet quality measured with dynamic light scattering correlates with transfusion outcome in hematologic malignancies

Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

“…In contrast, the DLS score combines all of these aspects, as well as the response to temperature stress to provide a score that has shown potential in estimating the in vivo efficacy of fresh liquidstored PLTs before transfusion. 22,39,40 While cold-stored PLTs had a lower DLS score than those stored at room temperature, all units maintained a score greater than 12, for up to 14 days of storage, which has been associated with better corrected count increments at 1 hour posttransfusion. 39,40 The lower score is likely partially reflective of the increase in microparticles, and while the algorithm assumes that microparticles are undesirable in PLT components, we have previously shown that they may actually provide a hemostatic benefit.…”

“…22,39,40 While cold-stored PLTs had a lower DLS score than those stored at room temperature, all units maintained a score greater than 12, for up to 14 days of storage, which has been associated with better corrected count increments at 1 hour posttransfusion. 39,40 The lower score is likely partially reflective of the increase in microparticles, and while the algorithm assumes that microparticles are undesirable in PLT components, we have previously shown that they may actually provide a hemostatic benefit. 16,21 The DLS score is also influenced by the response of PLTs to temperature stress 22 and may be indicative of posttransfusion efficacy, as is HSR, 25 which indicates membrane stress.…”

The influence of four different anticoagulants on dynamic light scattering of platelets

“…TLX uses dynamic light scattering (DLS) to determine the PLT size, relative number of microparticles and response of PLT to temperature stress . DLS uses the principles of Brownian motion and Doppler shift.…”

“…ThromboLUX ™ (LightIntegra, Vancouver, Canada) was introduced as a quick and easy applicable test device to determine platelet (PLT) quality prior to transfusion to predict PLT increment and in vivo quality . However, providing good PLT quality in PLT concentrates (PC) might already start with the blood donation.…”

What about Platelet Function in Platelet Concentrates?

“…Another interesting approach is the use of light scattering to monitor platelet shape change and microparticle content following temperature cycling. 43,44 A score can be obtained (including platelet shape as well as microparticle count), which represents the quality of the PCs. The idea of such type of control could be the elimination of "poor" PCs or the distribution of them in function of the score.…”