Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

Johnson, Lacey; Tan, Shereen; Wood, Ben; Davis, April; Marks, Denese C.

doi:10.1111/trf.13630

Cited by 117 publications

(230 citation statements)

References 60 publications

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“…A renewed interest in cold storage of platelets has shown that, as with cryopreserved platelets, cold storage leads to increased activation and more procoagulant function, with increased expression of activation markers such as CD62P and exposure of PS, improved in vitro TEG and ROTEM responses, more vigorous aggregation responses, and increased thrombin generation in comparison to room temperature-stored platelets [113, 114, 189–191]. As such, assays for measurement of in vitro function that are suitable for characterization of cryopreserved platelets, including TEG or ROTEM, CAT, microparticle enumeration, and flow cytometry, are also very useful for characterization of cold-stored platelets.…”

Section: Quality Of Platelet Concentratesmentioning

confidence: 99%

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Quality Assessment of Established and Emerging Blood Components for Transfusion

Acker

Marks

Sheffield

2016

Journal of Blood Transfusion

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Blood is donated either as whole blood, with subsequent component processing, or through the use of apheresis devices that extract one or more components and return the rest of the donation to the donor. Blood component therapy supplanted whole blood transfusion in industrialized countries in the middle of the twentieth century and remains the standard of care for the majority of patients receiving a transfusion. Traditionally, blood has been processed into three main blood products: red blood cell concentrates; platelet concentrates; and transfusable plasma. Ensuring that these products are of high quality and that they deliver their intended benefits to patients throughout their shelf-life is a complex task. Further complexity has been added with the development of products stored under nonstandard conditions or subjected to additional manufacturing steps (e.g., cryopreserved platelets, irradiated red cells, and lyophilized plasma). Here we review established and emerging methodologies for assessing blood product quality and address controversies and uncertainties in this thriving and active field of investigation.

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Section: Quality Of Platelet Concentratesmentioning

confidence: 99%

“…For example, cold storage of platelets slows glycolysis as well as attenuating acetate metabolism, possibly through inhibition of oxidative phosphorylation. Given that cold storage causes a change from discoid to spherical shape, measurements such as MPV, swirl, HSR, and ESC are also informative [113, 114, 192]. …”

Section: Quality Of Platelet Concentratesmentioning

confidence: 99%

Quality Assessment of Established and Emerging Blood Components for Transfusion

Acker

Marks

Sheffield

2016

Journal of Blood Transfusion

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“…In contrast, aged, chilled, or otherwise activated platelets are expected to be heterogeneous, containing platelets with high polydispersity due to a large spread of different morphologies, high surface expression of activation markers, many microparticles, and the presence of microaggregates [21, 28, 29] (Figure 1(b)). Heterogeneous platelets are known to be more functional but less viable.…”

Section: Platelet Quality From the Perspective Of Productionmentioning

confidence: 99%

“…Heterogeneous platelet concentrates contain preactivated platelets, which are fit to react quickly once they enter circulation [15, 21, 28]. Thus, heterogeneous platelets are highly functional and have been shown to stop bleeding faster than homogeneous, viable platelets [28].…”

Section: Platelet Quality For Therapeutic Usementioning

confidence: 99%

“…In this review, we explore the possibility that microparticle content as a measure of platelet fragmentation and heterogeneity may fulfill the requirement for a universal quality indicator for platelet production, storage, viability, function, and compatibility [19–21]. We assess the heterogeneity of platelet concentrates from the three different perspectives of production, prophylactic transfusion, and therapeutic transfusion.…”

Section: Introductionmentioning

confidence: 99%

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Could Microparticles Be the Universal Quality Indicator for Platelet Viability and Function?

Maurer‐Spurej

Chipperfield

2016

Journal of Blood Transfusion

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High quality means good fitness for the intended use. Research activity regarding quality measures for platelet transfusions has focused on platelet storage and platelet storage lesion. Thus, platelet quality is judged from the manufacturer's point of view and regulated to ensure consistency and stability of the manufacturing process. Assuming that fresh product is always superior to aged product, maintaining in vitro characteristics should preserve high quality. However, despite the highest in vitro quality standards, platelets often fail in vivo. This suggests we may need different quality measures to predict platelet performance after transfusion. Adding to this complexity, platelets are used clinically for very different purposes: platelets need to circulate when given as prophylaxis to cancer patients and to stop bleeding when given to surgery or trauma patients. In addition, the emerging application of platelet-rich plasma injections exploits the immunological functions of platelets. Requirements for quality of platelets intended to prevent bleeding, stop bleeding, or promote wound healing are potentially very different. Can a single measurable characteristic describe platelet quality for all uses? Here we present microparticle measurement in platelet samples, and its potential to become the universal quality characteristic for platelet production, storage, viability, function, and compatibility.

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The platelet storage lesion, what are we working for?

Liu,

Su,

Guo

et al. 2023

Clinical Laboratory Analysis

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BackgroundPlatelet concentrate (PC) transfusions are crucial in prevention and treatment of bleeding in infection, surgery, leukemia, and thrombocytopenia patients. Although the technology for platelet preparation and storage has evolved over the decades, there are still challenges in the demand for platelets in blood banks because the platelet shelf life is limited to 5 days due to bacterial contamination and platelet storage lesions (PSLs) at 20–24°C under constant horizontal agitation. In addition, the relations between some adverse effects of platelet transfusions and PSLs have also been considered. Therefore, understanding the mechanisms of PSLs is conducive to obtaining high quality platelets and facilitating safe and effective platelet transfusions.ObjectiveThis review summarizes developments in mechanistic research of PSLs and their relationship with clinical practice, providing insights for future research.MethodsAuthors conducted a search on PubMed and Web of Science using the professional terms “PSL” and “platelet transfusion.” The obtained literature was then roughly categorized based on their research content. Similar studies were grouped into the same sections, and further searches were conducted based on the keywords of each section.ResultsDifferent studies have explored PSLs from various perspectives, including changes in platelet morphology, surface molecules, biological response modifiers (BMRs), metabolism, and proteins and RNA, in an attempt to monitor PSLs and identify intervention targets that could alleviate PSLs. Moreover, novel platelet storage conditions, including platelet additive solutions (PAS) and reconsidered cold storage methods, are explored. There are two approaches to obtaining high‐quality platelets. One approach simulates the in vivo environment to maintain platelet activity, while the other keeps platelets at a low activity level in vitro under low temperatures.ConclusionUnderstanding PSLs helps us identify good intervention targets and assess the therapeutic effects of different PSLs stages for different patients.

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Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions

Abstract: Cold storage and cryopreservation of PLTs led to morphologic and metabolic changes. However, storage under these conditions appears to maintain or even enhance certain aspects of in vitro PLT function.

Cited by 117 publications

References 60 publications

Quality Assessment of Established and Emerging Blood Components for Transfusion

Quality Assessment of Established and Emerging Blood Components for Transfusion

Could Microparticles Be the Universal Quality Indicator for Platelet Viability and Function?

The platelet storage lesion, what are we working for?

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