Platelet Function, Size and Yield in Whole Blood and in Platelet-Rich Plasma Prepared Using Differing Centrifugation Force and Time in Domestic and Food-Producing Animals

Clemmons, Roger M.; Bliss, Eugene L.; Dorsey-Lee, M. R.; Seachord, Carrie L.; Meyers, Kenneth M.

doi:10.1055/s-0038-1665324

Cited by 44 publications

(27 citation statements)

References 13 publications

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“…Generally, the platelets of human and various mammalians (including rabbits) have a similar ultrastructure and constituents. 9,10 Advantages of using a rabbit as an animal model include the following: (1) easy manipulation, (2) sufficient volume of blood for the preparation of platelet concentrates, (3) survival of platelets similar to the metabolic activity of bone (one third of human), and (4) a high cross-reactivity of antihuman antibodies for immunohistochemical studies.…”

Section: Discussionmentioning

confidence: 99%

Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits

Kim

Park

Choung

2001

The Journal of Prosthetic Dentistry

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Statement of problem.The use of the platelet concentration technique is widespread in dental implant surgery. However, its effect or mechanism is not clearly understood.Purpose. This study introduced an animal model for the platelet concentration technique and evaluated its effect on bone formation with natural cancellous bovine bone mineral.Material and methods. Adult New Zealand white rabbits were used as the animal model. A density gradient medium was used to obtain a constant platelet count for the preparation of platelet concentrates. In the experimental group, natural cancellous bovine bone mineral with added platelet concentrates was grafted onto critically sized bony defects of the rabbit calvarium. Bone formation in the tissue sections was evaluated with soft x-ray imaging and computer tomography.Results. The average platelet count of the rabbit platelet concentrates was 1487 × 10 3 /µL (287% concentrated). In all the tested parameters, greater bone densities were obtained in grafts that were combined with platelet concentrates. Conclusion.This study showed that the rabbit is a useful animal model for studying the platelet concentration technique. When combined with grafts of natural cancellous bovine bone mineral, the technique increased bone formation. (J Prosthet Dent 2001;86:428-33.)

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Section: Discussionmentioning

confidence: 99%

Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits

Kim

Park

Choung

2001

The Journal of Prosthetic Dentistry

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“…22 Finally, 7.5 mL of PRP suspension was obtained ( Table 1). Counting of the platelet concentration was also performed (platelet count exceeding 800Â10 6 /mL).…”

Section: Procedures Of Prp Preparation and Applicationmentioning

confidence: 99%

The Effect of Platelet-Rich Plasma on the Bone Healing around Calcium Phosphate?Coated and Non-coated Oral Implants in Trabecular Bone

Nikolidakis¹,

Dolder²,

Wolke³

et al. 2006

Tissue Engineering

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The effect of local application of autologous platelet-rich plasma (PRP) on bone healing in combination with the use of titanium implants with 2 different surface configurations was investigated. PRP fractions were obtained from venous blood sample of 6 goats and applied via gel preparation and subsequent installation in the implant site or via dipping of the implant in PRP liquid before insertion. Thirty-six implants (18 non-coated and 18 calcium phosphate (CaP) coated) were placed into the goat femoral condyles (trabecular bone). The animals were sacrificed at 6 weeks after implantation, and implants with surrounding tissue were processed for light microscopical evaluation. In addition to subjective description of the histological findings, histomorphometrical variables were also evaluated (the boneimplant contact and the bone mass adjacent to the implant). Significantly more interfacial bone-to-implant contact was observed for all 3 groups of CaP-coated implants and the titanium / liquid group (non-coated implant with PRP liquid) than for the other 2 non-coated titanium groups (with PRP gel or without PRP). The evaluation of the bone mass close to implant surface indicated that all the groups induced a significant increase of the bone mass except the PRP gel groups. On the basis of the observations, it was concluded that magnetron-sputtered CaP coatings can improve the integration of oral implants in trabecular bone. The additional use of PRP did not offer any significant effect on the bone response to the CaP-coated implants, whereas PRP in a liquid form showed a significant effect on bone apposition to roughened titanium implants during the early post-implantation healing phase.

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“…Large animal models including pigs, sheep, goats, dogs, and rabbits have been used as surrogates to study the effects of surgical intervention after ACL injury [7,17,19,20,22,26,44]. Among those, the porcine model has been shown to be the closest to the human based on the size and anatomy of the knee [48], functional dependence on the ACL [10], gait biomechanics [60], and similarity of hematology and wound healing characteristics [15,38,40]. However, it is unknown if the porcine model also could be used to study sex-related phenomena associated with ACL injury.…”

Section: Introductionmentioning

confidence: 99%

Validation of Porcine Knee as a Sex-specific Model to Study Human Anterior Cruciate Ligament Disorders

Kiapour

Shalvoy

Murray

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Animal models have long been considered an important modality for studying ACL injuries. However, to our knowledge, the value of these preclinical models to study sex-related phenomena associated with ACL injury and recovery has not been evaluated. Questions/purposesWe asked whether (1) prominent anatomic and (2) biomechanical factors differ between female and male porcine knees, particularly those known to increase the risk of ACL injury. Methods Eighteen intact minipig knees (nine males, nine females) underwent MRI to determine the femoral bicondylar width, intercondylar notch size (width, area and index), medial and lateral tibial slope, ACL size (length, cross-sectional area, and volume), and medial compartment tibiofemoral cartilage thickness. AP knee laxity at 30°, 60°, and 90°flexion and ACL tensile structural properties were measured using custom-designed loading fixtures in a universal tensile testing apparatus. Comparisons between males and females were performed for all anatomic and biomechanical measures. The findings then were compared with published data from human knees. Results Female pigs had smaller bicondylar widths (2.9 mm, ratio = 0.93, effect size = À1.5) and intercondylar notches (width: 2.0 mm, ratio = 0.79, effect size = À2.8; area: 30.8 mm 2 , ratio = 0.76, effect size = 2.1; index: 0.4, ratio = 0.84, effect size = À2.0), steeper lateral tibial slope (4.3°, ratio = 1.13, effect size = 1.1), smaller ACL (length: 2.7 mm, ratio = 0.91, effect size = 1.1; area: 6.8 mm 2 , ratio = 0.74, effect size = À1.5; volume: 266.2 mm 3 , ratio = 0.68, effect size = À1.5), thinner medial femoral cartilage (0.4 mm, ratio = 0.8, effect size = À1.1), lower ACL yield load (275 N, ratio = 0.81, effect size = À1.1), and greater AP knee laxity at 30°( 0.7 mm, ratio = 1.32, effect size = 1.1) and 90°(0.5 mm, ratio = 1.24, effect size = 1

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Platelet Function, Size and Yield in Whole Blood and in Platelet-Rich Plasma Prepared Using Differing Centrifugation Force and Time in Domestic and Food-Producing Animals

Cited by 44 publications

References 13 publications

Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits

Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits

The Effect of Platelet-Rich Plasma on the Bone Healing around Calcium Phosphate?Coated and Non-coated Oral Implants in Trabecular Bone

Validation of Porcine Knee as a Sex-specific Model to Study Human Anterior Cruciate Ligament Disorders

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