Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits

Kim, Eun Seok; Park, Eun Jin; Choung, Pill Hoon

doi:10.1067/mpr.2001.115874

Cited by 91 publications

(56 citation statements)

References 9 publications

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“…After six months more bone was found in defects treated with PRP and radiographic examination showed enhanced maturity and bone consolidation of the PRP treated graft. Furthermore, PRP increased in combination with bovine cancellous bone allograft increased bone formation in calvarial defects in rabbit [6]. Several factors might explain why we found no effect of PRP.…”

Section: Discussioncontrasting

confidence: 49%

“…Those factors are important in initiating fracture healing. By concentrating platelets and delivering them locally, a higher level of growth factors might be reached which could stimulate the healing process in bone in a more physiological way than delivery of a single growth factor [6,15]. In a recent clinical study, PRP was capable of increasing bone regeneration in mandibular defects in combination with autograft [15].…”

Section: Introductionmentioning

confidence: 99%

“…In a recent clinical study, PRP was capable of increasing bone regeneration in mandibular defects in combination with autograft [15]. In rabbits PRP increased bone formation in combination bovine cancellous bone in calvarial defects [6].…”

Section: Introductionmentioning

confidence: 99%

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Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixationAn experimental study in dogs

JENSEN

2004

Journal of Orthopaedic Research

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Platelet rich plasma (PRP) is an autologous source of growth factors. By application of PRP around cementless implants alone or in combination with bone allograft chips, early implant fixation and gap healing could be improved.We inserted two porous HA coated titanium implants extraarticularly in each proximal humerus of eight dogs. Each implant was surrounded by a 2.5 mm gap. Four treatments were block randomized to the four gaps in each dog: Treatment 1: empty gap, treatment 2: PRP, treatment 3: fresh frozen bone allograft, treatment 4: fresh frozen bone allograft + PRP.PRP was prepared from each dog prior to operation by isolating the buffycoat from centrifuged blood samples. Platelet count in PRP was increased h70'%, compared to baseline level. Calciumlthrombin was added to degranulate platelets and form a gel.Three weeks after surgery, push-out test and histomorphometri was performed. After three weeks, the non-allografted implants had poor mechanical properties. Bone grafting significantly increased implant fixation. bone formation in the gap and bone growth on the implant surface. We found no significant effect of PRP alone or mixed with bone allograft on implant fixation or bone formation.In conclusion, we showed the importance of bone allografting on early implant fixation and bone incorporation but we found no effect of PRP. More studies are needed to investigate the effect and possible clinical applications of platelet concentrates which are now being commercialised.

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Section: Discussioncontrasting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

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Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixationAn experimental study in dogs

JENSEN

2004

Journal of Orthopaedic Research

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“…Dogs also were used in studies with PRP. Kim et al 25 demonstrate positive results in the association of implants, dried frozen bovine bone and PRP. Suba et al 27 associate the beta- 15 , and for Jensen et al 29 .…”

Section: Discussionmentioning

confidence: 99%

“…Some of these studies also use rabbits 5,25,26 . Aghaloo et al 5 show a very similar study to this presented here, with differences in the preparation of the PRP, in the monitoring periods (two, four and six weeks here and one, two and four months in theirs) and in the results when in the current study the PRP seemed to be harmful to the repair, these authors practically demonstrate neutral action of the PRP.…”

Section: Discussionmentioning

confidence: 99%

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

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Effects of autologous platelet lysates on ceramic particle resorption and new bone formation in critical size defects: The role of anatomical sites

Soffer

Ouhayoun²,

Meunier

et al. 2006

J Biomed Mater Res

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This study investigated the effects of rabbit autologous platelet lysates (APL) on the performance of fillers consisting of calcium carbonate ceramic particles (CP) pertinent to new bone formation and repair. Critical-size defects in rabbit femurs and calvaria were filled with CP alone, CP plus APL, and CP plus APL with or without thrombin (THR). After 6 weeks, resorption of CP occurred under all conditions tested in the present study. Compared with respective CP alone controls, addition of APL resulted in significantly higher ceramic resorption, as evidenced by decreased ceramic particle diameter (p < 0.01) and number (p < 0.01) at both defect sites. The presence of THR prevented reduction of both CP diameter and number in the femoral defect sites. Addition of APL to the CP resulted in a significant (p < 0.03) decrease in new bone area at the calvarial sites, but not at the femoral sites; moreover, when THR was added to the CP plus APL fillers, bone formation in the femoral defects was significantly (p < 0.05) reduced. In addition to differences in the respective anatomical and cellular milieu, the biochemical events induced by mechanical loading at the femurs may explain the reduced ceramic particle resorption as well as the enhanced new bone formation when compared with the results obtained at the calvarial defect sites.

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Platelet concentration and its effect on bone formation in calvarial defects: An experimental study in rabbits

Cited by 91 publications

References 9 publications

Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixationAn experimental study in dogs

Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixationAn experimental study in dogs

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

Effects of autologous platelet lysates on ceramic particle resorption and new bone formation in critical size defects: The role of anatomical sites

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