Plastotoma rotundifolium aerial tissue extract has antibacterial activities

Ngezahayo, Jeremie; Pottier, Laurent; Ribeiro, Sofia Oliveira; Delporte, Cédric; Fontaine, Véronique; Hari, Léonard; Stévigny, Caroline; Duez, Pierre

doi:10.1016/j.indcrop.2016.04.004

Cited by 15 publications

(14 citation statements)

References 39 publications

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“…Recent findings indicate that, beyond their curative effectiveness through a bactericide or bacteriostatic mode of action, plant bioactive constituents can also contribute to limit the development of bacteria within infected hosts through the disruption of bacterial virulence, undermining the strength of pathogenic bacteria [33,34]. Direct and indirect bactericidal properties of P. rotundifolium triterpenic acids (e.g., ursolic and corrosolic acids) against Gram-positive S. aureus (sensitive and methicillin-resistant) and Gram-negative E. coli have already been described by our group [16]. The present study indicates that the aerial part of P. rotundifolium exert anti-virulence rather than bactericidal activities towards the Gram-negative opportunistic pathogen P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Rasamiravaka

Ngezahayo

Pottier

et al. 2017

IJMS

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Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability. Bioguided fractionation of the ethyl acetate extract led to the isolation of major anti-virulence compounds that were identified from nuclear magnetic resonance and high-resolution molecular spectroscopy spectra as cassipourol, β-sitosterol and α-amyrin. Globally, cassipourol and β-sitosterol inhibit quorum sensing-regulated and -regulatory genes expression in las and rhl systems without affecting the global regulators gacA and vfr, whereas α-amyrin had no effect on the expression of these genes. These terpenoids disrupt the formation of biofilms at concentrations down to 12.5, 50 and 50 µM for cassipourol, β-sitosterol and α-amyrin, respectively. Moreover, these terpenoids reduce the production of total exopolysaccharides and promote flagella-dependent motilities (swimming and swarming). The isolated terpenoids exert a wide range of inhibition processes, suggesting a complex mechanism of action targeting P. aeruginosa virulence mechanisms which support the wide anti-infectious use of this plant species in traditional Burundian medicine.

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Section: Discussionmentioning

confidence: 99%

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Rasamiravaka

Ngezahayo

Pottier

et al. 2017

IJMS

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“…In the fight against these resistant pathogens, plant extracts heavily exploited in different civilizations (Amblard et al, 2007;Zuo et al, 2015) could yield clues to new therapeutic options (Okusa et al, 2014;Rasamiravaka Lim et al, 2010Lim et al, et al, 2014Ngezahayo et al, 2015;Ngezahayo et al, 2016). The antimicrobial activities of honokiol and magnolol, the main constituents of M. officinalis, were effective against Gram-positive bacteria, such as Streptococcus mutans (Bang et al, 2000) (Chang et al, 1998;Bang et al, 2000;Ho et al, 2001;Hu et al, 2011).…”

Section: Antimicrobial Activitiesmentioning

confidence: 99%

Biological activity and toxicity of the Chinese herb Magnolia officinalis Rehder & E. Wilson (Houpo) and its constituents

Poivre

Duez

2017

J. Zhejiang Univ. Sci. B

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106

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Traditional Chinese herbal drugs have been used for thousands of years in Chinese pharmacopoeia. The bark of Magnolia officinalis Rehder & E. Wilson, known under the pinyin name "Houpo", has been traditionally used in Chinese and Japanese medicines for the treatment of anxiety, asthma, depression, gastrointestinal disorders, headache, and more. Moreover, Magnolia bark extract is a major constituent of currently marketed dietary supplements and cosmetic products. Much pharmacological activity has been reported for this herb and its major compounds, notably antioxidant, anti-inflammatory, antibiotic and antispasmodic effects. However, the mechanisms underlying this have not been elucidated and only a very few clinical trials have been published. In vitro and in vivo toxicity studies have also been published and indicate some intriguing features. The present review aims to summarize the literature on M. officinalis bark composition, utilisation, pharmacology, and safety.

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“…This species is very promising for its antibacterial effect, classified as ‘very interesting’ [MICs < 100 μg/mL, according to Rios and Recio ()]. Our recent study indicates that this antibacterial activity could be due to acid pentacyclic triterpenoids (Ngezahayo et al ., ).…”

Section: Resultsmentioning

confidence: 97%

In vitro Study of Five Herbs Used Against Microbial Infections in Burundi

Ngezahayo

Ribeiro

Fontaine

et al. 2017

Phytotherapy Research

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The emergence of antimicrobial resistant infectious diseases remains a major threat to worldwide public health, in developed and in developing countries. Therefore, new antimicrobial agents acting by new mechanisms of action are urgently needed. As plants used in traditional medicine may help to overcome these problems, Justicia subsessilis, Platostoma rotundifolium, Pavetta ternifolia, Stomatanthes africanus, and Virectaria major (plants highly cited to be used against microbial infections in traditional Burundian medicine) were studied to assess their traditional use efficacy. We conducted a preliminary phytochemical screening of the extracts, as well as their direct and indirect (effect on antibiotic resistance) antibacterial activity on four bacterial strains (Staphylococcus sp. and Escherichia coli) by broth microdilution methods. All five medicinal plants investigated in this work were found to have direct antibacterial activity against all tested bacterial strains (minimum inhibitory concentration = 62.5-1000 μg/mL) that may support the use of these species in traditional Burundian medicine. Extracts (with no direct antibacterial activity), tested at 200 μg/mL, decreased the MIC values of β-lactams and aminoglycoside antibiotics by a factor of 2 to 64-fold. These interactions between plant extracts and antibiotics could open an avenue of research against antibiotic resistance. Copyright © 2017 John Wiley & Sons, Ltd.

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Plastotoma rotundifolium aerial tissue extract has antibacterial activities

Cited by 15 publications

References 39 publications

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Biological activity and toxicity of the Chinese herb Magnolia officinalis Rehder & E. Wilson (Houpo) and its constituents

In vitro Study of Five Herbs Used Against Microbial Infections in Burundi

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