Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

Abstract

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding can be reversed using chemical means (10 mM HCl). It is demonstrated that a multiplexed detection and identification of several fungal pathogen DNA sequences subsequently on one sensor array is possible by this approach.

Current and Perspective Diagnostic Techniques for COVID-19

“… 34 Another miRNA biosensor uses generic neutravidin combined with electrochemically encoded responsive nanolabels to measure the opening of molecular beacons by means of a square-wave stripping voltammetry. 35 The biosensor that combines cyclic amplification with hairpin DNA detects attomolar miRNA via surface plasmon-enhanced electrochemiluminescence. 36 The gold nanoparticle with an RNA three-way junction motif can sense miRNA using electrochemical surface-enhanced Raman spectroscopy.…”

“… 41 The plasmonic nanoparticle-based optical biosensor array functionalized with capture DNA binds to complementary viral RNA, which can be reversed by chemical treatment (10 mM HCl). 35 FET with a two-dimensional channel made of a single graphene layer achieves label-free detection of RNA down to attomolar concentration. 42 Single-molecule fluorescence resonance energy transfer remains a promising tool for multiplex viral RNA detection (∼200 pM) with interconvertible hairpin-based sensors.…”

A miRNA biosensor based on localized surface plasmon resonance enhanced by surface-bound hybridization chain reaction

“…We obtained the enhancement of a previously developed LSPR nucleic acid biosensor ( Thamm et al, 2018 ; Zopf et al, 2019 ) by integrating surface HCR on it. This biosensor was reproduced by immobilizing 80 nm spherical gold nanoparticles on glass slides.…”

“…1 B). After each measurement cycle, the biosensor surface was regenerated by flowing 20 mM HCl ( Zopf et al, 2019 ) and verifying that the baseline centroid location was obtained again. A typical calibration curve of the system response is displayed in Fig.…”

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

“…Plasmonic microarrays are integrated in a microfluidic chamber. Reproduced with permission from Zopf et al, American Chemical Society [ 83 ]. d Handheld device developed for the detection of Hg 2+ , using aptamer-templated ZnO QDs.…”