A miRNA biosensor based on localized surface plasmon resonance enhanced by surface-bound hybridization chain reaction

Abstract

The dysregulation of the concentration of individual circulating microRNAs or small sets of them has been recognized as a marker of disease. For example, an increase of the concentration of circulating miR-17 has been linked to lung cancer and metastatic breast cancer, while its decrease has been found in multiple sclerosis and gastric cancer. Consequently, techniques for the fast, specific and simple quantitation of microRNAs are becoming crucial enablers of early diagnosis and therapeutic follow-up. DNA based biosensors can serve this purpose, overcoming some of the drawbacks of conventional lab-based techniques. Herein, we report a cost-effective, simple and robust biosensor based on localized surface plasmon resonance and hybridization chain reaction. Immobilized gold nanoparticles are used for the detection of miR-17. Specificity of the detection was achieved by the use of hairpin surface-tethered probes and the hybridization chain reaction was used to amplify the detection signal and thus extend the dynamic range of the quantitation. Less than 1 h is needed for the entire procedure that achieved a limit of detection of about 1 pM or 50 amol/measurement, well within the reported useful range for diagnostic applications. We suggest that this technology could be a promising substitute of traditional lab-based techniques for the detection and quantification of miRNAs after these are extracted from diagnostic specimens and their analysis is thus made possible.

High-Sensitivity High-Throughput Detection of Nucleic Acid Targets on Metasurface Fluorescence Biosensors

“…Extremely high sensitivity of 100 aM for microRNA (miRNA) was claimed based on the measured resonant wavelength shift on local plasmon resonance [ 55 ]; the miRNA detection went through a long process such that cell culture took 6 days, transduction and extraction of the miRNA took further 4 days, the isolation needed more than 100 min, and incubation on gold nanostructure occurred overnight; in total, a week was needed for one run. In contrast, a high-throughput sensing [ 56 ] conducted at 1 h for miRNA based on the resonant wavelength shift, surface-bound hybridization chain reaction was incorporated; the LOD was 1 pM. Thus, high throughput and high sensitivity are trade-off.…”

Trends in biosensing platforms for SARS-CoV-2 detection: A critical appraisal against standard detection tools

“… Biosensor Pathogen Detection target Limit of detection Linear range Ref. EC SARS-CoV-2 S or N protein 19 ng/mL and 8 ng/mL - [ 103 ] EC SARS-CoV-2 Antibodies - - [ 101 ] EC SARS-CoV-2 N-gene 6.9 copies/μL 585.4 to 5.854 × 10 7 copies/μL [ 99 ] Cell-based SARS-CoV-2 Antigen 1 fgmL 1- 10 fg and 1 μg mL 1- [ 105 ] Optofluidic SARS-CoV-2 Antibody 0.5 pM 1- [ 83 ] Nanoplasmonic SARS-CoV-2 Virus particles 370 vp/mL 0 to 10 7 vp/mL [ 80 ] SPR SARS-CoV-2 Antibody 1.02 pM 2-1000 pM [ 82 ] LSPR SARS-CoV-2 RNA 1 pM 1 nM to 1 μM [ 106 ] Lateral flow optical/chemiluminescence SARS-CoV-2 Serum IgA - - [ 78 ] SERS-LFIA SARS-CoV-2 Antibody 1.28×10 7 -fold dilution - [ 79 ] Lateral flow SARS-CoV-2 RNA 12 copies...…”

CRP Detection Using Gold Nanosensors – Effect of Layer Thickness and Immobilization Chemistry

“…For all the micro uidic assays a custom build LSPR instrument was used. It consists of a halogen light source HL − 2000 − FHSA (Ocean Optics, USA), an optical ber connected UV/VIS linear photodiode array spectrometer USB 2000+ (Ocean Optics, USA), a peristaltic pump (Is-matec Reglo-ICC, Cole-Parmer GmbH, Wertheim, Germany), a 2-way valve (Bio-Chem Fluidics Inc, Boonton, USA) for the waste and a custom designed 3D printed micro uidic chamber and sealing with two inputs and one output capillary at each channel side similar to an earlier published setup [25][26][27] . For some measurements single-use ow cells "Basic sensor platform II" (# 10001354, micro uidic chip shop GmbH, Jena, Germany) were used with adhesive tape gasket for Fl.…”