Plasma protein thiolation index (PTI) as a biomarker of thiol-specific oxidative stress in haemodialyzed patients

Colombo, Graziano; Reggiani, Francesco; Podestà, Manuel Alfredo; Garavaglia, Maria Lisa; Portinaro, Nicola; Milzani, Aldo; Badalamenti, Salvatore; Dalle‐Donne, Isabella

doi:10.1016/j.freeradbiomed.2015.08.022

Cited by 25 publications

(35 citation statements)

References 71 publications

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“…These results indicate that the level of oxidative stress is higher in patients with ESRD than in healthy individuals. Our findings are consistent with previous reports wherein patients with ESRD have been shown to have decreased plasma protein thiols and increased S-thiolated proteins (9,13,14). In addition, Przemysław et al (14) found that the total concentration of glutathione, which is a low molecular weight thiol-tripeptide, was decreased in terminal renal failure patients and that total concentrations of the remaining thiols in these patients were significantly increased compared to those in the healthy subjects.…”

Section: Discussionsupporting

confidence: 93%

“…Plasma protein thiols located primarily on albumin are depleted in patients with ESRD, and oxidized thiols that include homocysteine and cysteine accumulate, leading to the oxidation of reduced free and protein-bound thiols (4). An impaired homeostasis of blood thiols has been observed in ESRD patients, especially in those who have previously received HD therapy (9)(10)(11)). …”

Section: Introductionmentioning

confidence: 99%

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Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Budak¹,

Kahvecioğlu²,

Çelik³

et al. 2017

Turk Neph Dial Transpl

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OBJECTIVE:We aimed to evaluate thiol/disulfide homeostasis as an oxidative stress parameter in end-stage renal disease patients receiving different substitutive therapies. MATERIAL and METHODS:Twenty-four renal transplantation (46.2±8.1 years), 24 peritoneal dialysis (47.8±8.5 years), and 25 hemodialysis patients (45.7±16.0 years), and 24 healthy controls (47.6±8.1 years) were included. Serum native thiol, total thiol, and disulfide levels were measured for all subjects. RESULTS:Serum total thiol and native thiol levels in the ESRD patients were significantly lower than those in the controls (P<0.001). Native and total thiol levels were significantly lower in PD (256 ± 45 μmol/L, and 287 ± 57 μmol/L) compared to HD (324 ± 44 μmol/L and 356 ± 46 μmol/L) group (p<0.01 and p=0.03). Disulfide/native thiol and disulfide/total thiol levels increased significantly in the PD and transplantation patients compared to control group (P<0.001). Positive correlation was observed between total thiols (r= 0.491 P<0.001), disulfide/native thiol ratio (r=0.383, P<0.001), and blood urea nitrogen; disulfide levels and blood urea nitrogen showed a negative correlation (-0.415, P<0.001). Disulfide levels and glomerular filtration rate showed a positive correlation (r=0.276, P=0.030). CONCLUSION:Serum thiol/disulfide homeostasis can be an indicator of oxidative stress in end-stage renal disease and that thiol/disulfide homeostasis analysis should be included in the routine monitoring of end-stage renal disease patients. KEy wORDS: Hemodialysis, Peritoneal dialysis, Renal transplantationÖz AMAÇ: Çalışmada son dönem böbrek yetmezliği hastalığı için farklı renal replasman tedavisi alan hastalarda bir oksidatif stres belirteci olarak tiol/disülfit dengesinin araştırılması amaçlanmıştır. GEREÇ ve yÖNTEMLER:Yirmi dört böbrek nakli yapılmış (46,2±8,1 yaş), 25 hemodiyaliz (45,7±16,0 yaş) ve 24 periton diyalizi (47,8±8,5 yaş) hastaları ile 24 sağlıklı kontrol (47,6±8,1 yaş) çalışmaya dahil edildi. Tüm olgularda nativ tiyol, total tiyol ve disülfit seviyeleri ölçüldü. BULGULAR:Hastalarda kontrol grubu ile karşılaştırıldığında total tiyol ve nativ tiyol seviyeleri anlamlı olarak düşüktü (p<0,001). Nativ ve total tiyol düzeyi periton diyalizi hastalarında (256±45 μmol/L ve 287±57 μmol/L) hemodiyaliz hastalarına (324±44 μmol/L ve 356±46 μmol/L) göre anlamlı düşüktü (p<0,01 ve p=0,03). Periton diyalizi ve transplantasyon hastalarında disulfit/nativ tiyol ve disülfit/total tiyol düzeyleri kontrol grubuna gore anlamlı olarak yüksek bulundu (p<0,001). Total tiyol (r=0,491 P<0,001) ve disulfit/nativ tiyol oranı (r=0,383, P<0,001) ile kan üre nitrojeni arasında pozitif korelasyon; disülfit seviyeleri ve kan üre nitrojeni arasında negatif korelasyon (-0,415, P<0,001) saptandı. Disülfit seviyeleri ve glomerülar filtrasyon değeri arasında pozitif korelasyon (r=0,276, P=0,030) bulundu. SONUÇ:Tedavi alan son dönem böbrek hastalarında rutin hasta takibinde serum tiyol/disülfit oranı oksidatif stres belirteci olarak kullanılabilir.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Budak¹,

Kahvecioğlu²,

Çelik³

et al. 2017

Turk Neph Dial Transpl

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“…Decreased plasma protein thiols and increased S-thiolated proteins have previously been shown in end-stage renal disease patients (13)(14)(15).…”

Section: Introductionmentioning

confidence: 80%

Thiol/Disulphide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Single Center, Cross-Sectional Study

Esen¹,

Kahvecioğlu²,

Üstündağ³

et al. 2018

Turk Neph Dial Transpl

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OBJECTIVE:The aim of this study was to determine a novel oxidative stress marker, plasma thioldisulphide homoeostasis, in patients with autosomal dominant polycystic kidney disease (APKD). MATERIAL and METHODS:Thirty-four patients with autosomal dominant polycystic kidney disease and 30 healthy volunteers were included in the study. The thiol-disulfide homeostasis assay was performed. Total antioxidant capacity was evaluated via the FRAP assay. RESULTS:The median age was 46 (13) years in the patient group and 41 (13) years in the control group. In the APKD disease group, serum total and native thiol levels were statistically significantly lower (p = 0.002, p = 0.002, respectively). Serum native thiol levels were negatively correlated with age (r:-0.620 p: 0,000), systolic blood pressure (r: -0,697 p: 0,000), and diastolic blood pressure (r: -0,643 p: 0,000). A weak positive correlation was found between the disulfide/native thiol ratio and creatinine (r: 0.564 p <0.001), and a negative correlation was found between the disulfide/native thiol ratio and glomerular filtration rate (r: -0.372 p: 0.030). There was a significant positive correlation between the native thiol level and GFR (r: 0.699, p <0.001) level. CONCLUSION:Our findings suggest that thiol/disulfide homeostasis may be associated with autosomal dominant polycystic kidney disease progression. KEY WORDS:Oxidative stress, Autosomal dominant polycystic kidney disease, Thiol-disulphide, Creatinine ÖZ AMAÇ: Bu çalışmanın amacı, otozomal dominant polikistik böbrek (ODPK) hastalığı olanlarda yeni bir oksidatif stres markeri olan plazma tiyol-disülfid homeostazisini belirlemektir. GEREÇ ve YÖNTEMLER:Otozomal dominant polikistik böbrek hastalığı olan 34 hasta ve 30 sağlıklı gönüllü çalışmaya dahil edildi. Tiyol-disülfid homeostazı ölçüldü. Total antioksidan kapasite FRAP testi ile değerlendirildi. BULGULAR:Hastaların medyan yaşı 46 (13) yıl, kontrol grubunda 41 (13) yıldır. ODPK hastalarında serum total ve nativ tiyol düzeyleri istatistiksel olarak anlamlı derecede düşüktü (sırasıyla p=0,002, p=0,002). Serum nativ tiyol düzeyleri yaş (r:-0,620 p:0,000), sistolik kan basıncı (r:-0,697 p:0,000) ve diyastolik kan basıncıyla (r:-0,643 p:0,000) negatif korelasyon gösterdi. Disülfid / nativ tiyol oranı ile kreatinin arasında (r:0,564 p <0,001) zayıf pozitif korelasyon bulundu, disülfid / nativ tiyol oranı ve glomerüler filtrasyon hızı arasında negatif bir korelasyon bulundu (r:-0,372, p:0,030. Nativ tiyol düzeyi ile GFR arasında pozitif korelasyon bulundu (r:0,699, p<0,001). SONUÇ:Bulgularımız tiyol/disülfid homeostazının otozomal dominant polikistik böbrek hastalığının ilerlemesi ile ilişkili olabileceğini düşündürmektedir.

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“…The thiol molecules have been reported to scavenge free radicals and also play a role in detoxification, signal transduction and apoptosis [54]. Therefore, decreased levels of thiols have been found in some diseases associated with renal, cardiovascular and several other organ dysfunction [55]. In this study, IIRI decreased the level of both protein and non-protein thiols in the kidneys and heart while AI and vitamin C pre-treatment improved the levels of the thiols in both the heart and kidneys.…”

Section: Discussionmentioning

confidence: 99%

Preconditioning with Azadirachta indica ameliorates cardiorenal dysfunction through reduction in oxidative stress and extracellular signal regulated protein kinase signalling

Omóbòwálé

Oyagbemi

Adejumobi

et al. 2016

Journal of Ayurveda and Integrative Medicine

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BackgroundAzadirachta indica is widely distributed in Africa, Asia and other tropical parts of the world. A. indica (AI) is traditionally used for the treatment of several conditions including cancer, hypertension, heart diseases and skin disorders. Intestinal ischaemia-reperfusion is a common pathway for many diseases and may lead to multiple organ dysfunction syndrome and death.ObjectiveIn this study, we investigated the ameliorative effects of AI on intestinal ischaemia-reperfusion injury-induced cardiorenal dysfunction.Materials and methodsSixty rats were divided into 6 groups; each containing 10. Corn oil was orally administered to group A (control) rats for 7 days without intestinal ischaemia-reperfusion injury. Group B underwent intestinal ischaemia-reperfusion injury (IIRI) without any pre-treatment. Groups C, D, E and F were pre-treated orally for 7 days with 100 mg/kg AI (100 and (200 mg/kg) vitamin C (100 and 200 mg/kg) respectively and thereafter underwent IIRI on the 8th day.ResultsThe cardiac and renal hydrogen peroxide increased significantly whereas serum xanthine oxidase and myeloperoxidase levels were significantly elevated (p < 0.05) in IIRI only when compared to the control. The cardiac and renal reduced glutathione, glutathione peroxidase, protein thiol, non-protein thiol and serum nitric oxide (NO) decreased (p < 0.05) significantly following IIRI. Immunohistochemical evaluation of cardiac and renal tissues showed reduced expressions of the extracellular signal regulated kinase (ERK1/2) in rats with IIRI only. However, pre-treatment with A. indica and vitamin C significantly reduced markers of oxidative stress and inflammation together with improvement in antioxidant status. Also, reduced serum NO level was normalised in rats pre-treated with A. indica and vitamin C with concomitant higher expressions of cardiac and renal ERK1/2.ConclusionsTogether, A. indica and vitamin C prevented IRI-induced cardiorenal dysfunction via reduction in oxidative stress, improvement in antioxidant defence system and increase in the ERK1/2 expressions. Therefore, A. indica can be a useful chemopreventive agent in the prevention and treatment of conditions associated with intestinal ischaemia-reperfusion injury.

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Plasma protein thiolation index (PTI) as a biomarker of thiol-specific oxidative stress in haemodialyzed patients

Cited by 25 publications

References 71 publications

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Thiol/Disulphide Homeostasis in Autosomal Dominant Polycystic Kidney Disease: A Single Center, Cross-Sectional Study

Preconditioning with Azadirachta indica ameliorates cardiorenal dysfunction through reduction in oxidative stress and extracellular signal regulated protein kinase signalling

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