Preconditioning with Azadirachta indica ameliorates cardiorenal dysfunction through reduction in oxidative stress and extracellular signal regulated protein kinase signalling

Omóbòwálé, Temidayo Olutayo; Oyagbemi, Ademola Adetokunbo; Adejumobi, Olumuyiwa Abiola; Orherhe, Eguonor Vivian; Amid, S.A.; Adedapo, Adeolu Alex; Nottidge, Helen Oyebukola; Yakubu, Momoh Audu

doi:10.1016/j.jaim.2016.08.006

Cited by 14 publications

(5 citation statements)

References 58 publications

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“…Laha and Ezeigwe et al demonstrated that Gymnema sylvestre and extract of A. indica have pancreatic islet regeneration properties in diabetic mice 13,19 . The potential effects observed when using these extracts can certainly be ascribed to cellular mechanisms, which include scavenging of free radical, DNA repair, cell cycle alteration, programmed cell death mitigation, and the ability to modulate various signaling pathways 28,29 .…”

Section: Fig 6: Representative Images Of Cell Cycle Analysis Using Fl...mentioning

confidence: 99%

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2022

IJPSR

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The major pathogenic mechanisms of diabetes are chronic hyperglycemia and glucotoxicity. Excess production of reactive oxygen species due to hyperglycemia results in oxidative stress and changes in mitochondrial energy metabolism promote β cell dysfunction, eventually leading to apoptosis and the development of diabetic complications. Although several oral antihyperglycemic agents are available, their adverse effect profile affects their prolonged use. Medicinal plants, having a low toxicity profile, are being looked at as alternatives for diabetes treatment. The present study was thus planned to elucidate the mechanisms of action of 5 medicinal plantsviz. Azadirachta indica, Momordica charantia, Gymnema sylvestre, Syzygium cumini, and Trigonella foenum graecum selected on the basis of Ayurveda in diabetes using RINm5F cells. The effects of these plants on Streptozotocin (STZ) induced oxidative stress was assessed using variables like lipid peroxidation in terms of malondialdehyde (MDA) release, modulation of apoptosis, cell cycle analysis using flowcytometry, and insulin release using commercially available rat insulin Elisa kits. All the plant extracts in the presence of STZ inhibited the release of MDA as compared to STZ-treated cells. Maximum reduction was observed with Azadirachta indica and Gymnema sylvestre extracts compared to STZ treated cells, suggesting the ability to protect from cell death. Maximum and significant insulin release was observed at 200 µg/ml of Syzygium cumini followed by Gymnema sylvestre and Momordica charantia as compared to STZ treated cells. Our findings demonstrate that these plants have the potential to modulate diabetic conditions through their anti-oxidant property, anti-apoptotic and cytoprotective actions.

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Section: Fig 6: Representative Images Of Cell Cycle Analysis Using Fl...mentioning

confidence: 99%

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2022

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“…Increase in glutathione levels, better activity of the enzyme G-6-PD. X [3] Bark Powdered bark (20 g). Showed ethanolic extract has the highest content of flavonoids and phenols.…”

Section: Anti-inflammatory Undefinedmentioning

confidence: 99%

“…In a similar fashion, nitric oxide levels were maintained (control 0.036 mole/l, extract 0.034 mole/l and vit c 0.042 mole/    l), but diminished for the IIRI (0.025 mole/l).  Furthermore, levels of GSH were increased resulting in high recovery of the glucose-6-phosphate dehydrogenase (G6PD), therefore boosting the body's natural defenses [3]. Furthermore, studies by Ghatule et al, used acetic acid to induce colitis in rats.…”

Section: Antioxidant Effectmentioning

confidence: 99%

Current Evidence about Neem Extracts, the Molecules and Physiological Mechanisms Their Nutritional Components Interact with

Islas¹,

Acosta²,

Escalante-Acosta³

et al. 2019

Preprint

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Plant-based alternative medicine is normally grounded on empiric cultural perception, as the main objective, these practices intent to either maintain good health or to provide a route to turn-the-tide on a specific disease or ailment. Amongst the thousands of plants that have been used and studied, Neem (Azadirachta indica) seems to have a very interesting tale to tell, since its properties to ward-off certain diseases have overtime, and in a rigorous way, been proven. The preceding concise review is a collection of some of the most relevant studies today, not only focusing on the health benefits obtained by its use, but digging into the molecular mechanisms of how the properties come about. In particular, we take a look over antioxidant properties and how these mediate and mitigate important molecules such as IL-6 and TNF-, leading the way in reducing systematic damage by oxidative stress. Further, we relate this oxidative reduction to other systemic diseases such as cancer and diabetes, as these are currently becoming the most rampant killers. As of yet not all is known about the different ways of extracting or the total composition of an extract, as these may be from different parts of the plant. Therefore, we also allude to an important cautionary view where toxicological effects and conflicting outcomes arise. Overall, presented results show a great potential for the different extracts of Neem as their antioxidant activity can be taken advantage off, and potentially used in modern medicine.

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“…34 The potential effects that are seen when using A. indica extracts can positively be attributed to cellular and molecular mechanisms, which comprise detoxication, free radical scavenging, DNA repair, immune surveillance, programmed autophagy and cell death mitigation, cell cycle alteration, anti-inammatory, anti-metastatic and antiangiogenic activities and the capability to modulate different signaling pathways. 35,36 The anti-inammatory activity of NLP is due to the presence of the bioactive compound limonoid. 37,38 This research article addressed the fabrication of costeffective and eco-friendly bio-composites based on sulfatedcarboxymethyl cellulose (S-CMC) and the A. indica leaves (NLP) through a simple and green chemical approach.…”

Section: Introductionmentioning

confidence: 99%