Plasma Levels of miR-125b-5p and miR-206 in Acute Ischemic Stroke Patients After Recanalization Treatment: A Prospective Observational Study

Abstract

Introduction: Multiple microRNAs (miRNAs) participate in the response to hypoxic/ ischemic and ischemia-reperfusion events. However, the expression of these miRNAs in circulation from patients with acute ischemic stroke (AIS) receiving recanalization treatment has not been examined, and whether they are associated with the severity and outcome of stroke is still unknown. Materials and methods: In this prospective cohort study, plasma levels of miR-125b-5p, miR-15a-3p, miR-15a-5p, and miR-206 were measured at 24 hours after thrombolysis with or without endovascular treatment in 94 patients with AIS, as determined by qRT-PCR. Stroke severity was assessed based on National Institutes of Health Stroke Scale (NIHSS) score and infarct lesion. Intracranial haemorrhage (ICH) was recorded. An unfavorable outcome was defined as a modified Rankin Scale score greater than 2 at day 90 after stroke. Results: miR-125b-5p and miR-206 levels were correlated with NIHSS scores (P = .014 and P = .002) and cerebral infarction volumes (P = .025 and P = .030). miR-125b-5p levels were significantly higher in patients with an unfavorable outcome than in patients with a favorable outcome (P = .002) and showed good diagnostic accuracy in discriminating the presence of an unfavorable outcome (area under the curve .735, 95% confidence interval .623-.829, P < .001). No association was found between different miRNAs and ICH. Conclusions: In AIS patients after thrombolysis with or without endovascular treatment, miR-125b-5p is a novel prognostic biomarker highly associated with an unfavorable outcome. miR-125b-5p and miR-206 levels are associated with stroke severity.

Serum microRNA miR-491-5p/miR-206 Is Correlated with Poor Outcomes/Spontaneous Hemorrhagic Transformation after Ischemic Stroke: A Case Control Study

“…MiR-206, a muscle-enriched miRNA (myomiR), has an important role in myogenic differentiation [ 28 ]. MiR-206 was reported to be positively correlated with stroke severity [ 29 , 30 ] in humans and infarct volume in an animal model of embolic stroke [ 31 ], serving as a detrimental biomarker. Overexpression of miR-206 inhibits neuronal cell viability and proliferation, interfering with neurodevelopment [ 32 ].…”

Serum miRNA125a-5p, miR-125b-5p, and miR-433-5p as biomarkers to differentiate between posterior circulation stroke and peripheral vertigo

“…An in vivo experiment demonstrated that miR-125a-5p regulates blood brain barrier function [14]. MiR-125b-5p has previously been shown to be a predictive biomarker candidate for ischemic stroke outcomes [15,16]. MiR-433 is highly expressed in the brain tissue [11].…”

Current status of ischemic stroke treatment: From thrombolysis to potential regenerative medicine

“…Bone marrow derived mesenchymal stem cells EVs have been reported to home in the infarct area after transplantation and have therapeutic effects such as endogenous neurogenesis, increase the number of axons, and improve vital functions of animals [ 63 , 64 ]. When incorporated with different miRNAs, MSC-derived EVs were able to promote a neuroprotective response [ [65] , [66] , [67] ]. In some studies, MSCs overexpressing specific miRNAs were chosen and were able to be passed on to the daughter EVs.…”