Plasma hormonal profiles and dendritic spine density and morphology in the hippocampal CA1 stratum radiatum, evidenced by light microscopy, of virgin and postpartum female rats

Brusco, Janaína; Wittmann, Raul; Azevedo, Márcia Scherem de; Lucion, Aldo Bolten; Franci, Celso Rodrigues; Giovenardi, Márcia; Rasia‐Filho, Alberto A.

doi:10.1016/j.neulet.2008.04.063

Cited by 54 publications

(32 citation statements)

References 26 publications

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“…In the MePD of normally cycling females, plastic changes in the neuronal morphology (RasiaFilho et al, 2004) and neurochemical switches (e.g., changes in the local cholecystokinin immunoreactivity) occur across the estrous cycle (De Vries and Simerly, 2002; see a parallel discussion in Brusco et al, 2008). Neurons in the male MePD respond to olfactory stimulation and project to the ventral premammillary nucleus, where information from the perifornical area and from leptin regarding nutrition is integrated with information of the reproductive status (Cavalcante et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Glutamate Microinjected in the Posterodorsal Medial Amygdala Induces Subtle Increase in the Consumption of a Three‐Choice Macronutrient Self‐Selection Diet in Male Rats

Rosa¹,

Goularte²,

Trindade³

et al. 2011

The Anatomical Record

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Previous studies have involved the ''posterodorsal'' amygdaloid area with the control of food intake and the development of obesity in rats. Within this wide region, the posterodorsal medial amygdala (MePD) has connections with specific hypothalamic nuclei that increase feeding behavior and modulate energy balance. Glutamate is the major brain excitatory neurotransmitter, remarkably enhances centrally mediated food consumption, and is abundantly found in the MePD. Here, it was studied the effects of saline (0.3 lL) and glutamate (45 nM or 45 mM/0.3 lL) directly microinjected in the MePD of adult male rats on the consumption of a three-choice (high-carbohydrate, high-protein, or high-lipid) macronutrient selective diet. The rat adaptation to the experimental procedures and its body weight gain were continuously evaluated. Control data for all groups and results following microinjections were obtained after a fasting protocol. Feeding behavior was evaluated during the subsequent 2-hr period of free access to the selective diets. Both doses of glutamate microinjected in the MePD did not lead to a higher percentage of animals consuming any of the different diets (P > 0.05), although glutamate 45 mM induced a higher consumption of the high-carbohydrate diet when compared with presurgery control values (P < 0.01). Interestingly, present data indicate that glutamate in the male MePD induces only a subtle modification in the feeding behavior and suggest that large electrolytic lesions of the ''posterodorsal'' amygdaloid region might have affected other regions to alter drastically meal size consumption in rats. Anat Rec, 294:1226Rec, 294: -1232Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Glutamate Microinjected in the Posterodorsal Medial Amygdala Induces Subtle Increase in the Consumption of a Three‐Choice Macronutrient Self‐Selection Diet in Male Rats

Rosa¹,

Goularte²,

Trindade³

et al. 2011

The Anatomical Record

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“…Contrarily, proteins MAP and Tau, involved in the development of dendrites and axons respectively, do not change in frontal cortex as a consequence of E2 or P treatment [45], or due to hormonal variations along the estrous cycle [46]. Similarly, density of dendritic spines in the hippocampal CA1 stratum radiatum, seems to be not related with the high levels of estrogens reached during proestrous [47]. These data support the idea that estrogenic actions on dendritic architecture is regulated by several factors including the time elapsed after OVX and the connectivity and afferent activity of some particular brain region.…”

Section: Discussionmentioning

confidence: 90%

Morphological Changes Induced by the Absence of Ovarian Hormones in Nucleus Accumbens of Ovariectomized Rats

Quiñones¹,

Camacho-Ábrego²,

Rivera³

et al. 2009

TONEUROEJ

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Ovariectomy (OVX) has been widely used to explore the effect of ovarian hormones on brain regions. It has been observed that estrogen in OVX rats increases the dopaminergic function in nucleus accumbens (NAcc). To determine whether absence or presence of estradiol changes the dendritic structure of some dopaminergic neurons, we investigated the consequences of a daily injection for 2 weeks of 17-beta estradiol (E2) on the dendritic morphology of NAcc neurons in two groups of rats, immediately or 10 weeks administered after OVX. The structure of dendrites was measured by using the Golgi-Cox procedure followed by a Sholl analysis. We found a reduction in the number of spiny dendrites of the medium spiny neurons of the NAcc in animals in which E2 was administrated without delay after OVX. However, when the same treatment was administered 10 weeks after OVX, not any difference was observed. Independently of the hormone treatment, rats from the group of 10 weeks after OVX showed a reduction in the number of spiny dendrites of the medium spiny neurons when compared with their counterparts at 2 weeks. Data suggest that estrogens or their absence regulate synaptogenesis in the NAcc.

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“…It is relatively easy and fast (5-11 days), requires minimal equipments, and provides good results for human brain tissue fixed in formalin. Qualitative and quantitative studies can be conducted in well-impregnated neurons under light microscopy (see examples in Rasia-Filho et al, 2004;de Castilhos et al, 2006de Castilhos et al, , 2008Brusco et al, 2008;Dall'Oglio et al, 2008a,b) to be correlated with other neuronal and glial morphological and functional findings Martinez et al, 2006;Cunningham et al, 2007;de Castilhos et al, 2008;Rasia-Filho et al, 2009). Therefore, besides other current techniques and innovations, the Golgi method continues to be a relevant tool to neuroscience.…”

Section: Discussionmentioning

confidence: 99%

“…Soon after, Gabbott and Somogyi (1984) depicted the "single-section" Golgi method that proved to be easy, reliable, and relatively unexpensive. This approach has been successfully applied to clue the neuroendocrine modulation of the number of dendritic spines in different brain areas of male and female rats (Woolley et al, 1990;Woolley and McEwen, 1994;Rasia-Filho et al, 1999Brusco et al, 2008). It consists in sectioning the brain perfused with a paraformaldehyde and picric acid solution, placing the 60-200 m thick sections in potassium bichromate, and "sandwich" them between cover slips for the impregnation in silver nitrate.…”

Section: Introductionmentioning

confidence: 99%

The “single-section” Golgi method adapted for formalin-fixed human brain and light microscopy

Dall'Oglio¹,

Ferme²,

Brusco³

et al. 2010

Journal of Neuroscience Methods

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Plasma hormonal profiles and dendritic spine density and morphology in the hippocampal CA1 stratum radiatum, evidenced by light microscopy, of virgin and postpartum female rats

Cited by 54 publications

References 26 publications

Glutamate Microinjected in the Posterodorsal Medial Amygdala Induces Subtle Increase in the Consumption of a Three‐Choice Macronutrient Self‐Selection Diet in Male Rats

Glutamate Microinjected in the Posterodorsal Medial Amygdala Induces Subtle Increase in the Consumption of a Three‐Choice Macronutrient Self‐Selection Diet in Male Rats

Morphological Changes Induced by the Absence of Ovarian Hormones in Nucleus Accumbens of Ovariectomized Rats

The “single-section” Golgi method adapted for formalin-fixed human brain and light microscopy

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