The “single-section” Golgi method adapted for formalin-fixed human brain and light microscopy

Dall'Oglio, Aline; Ferme, Denise; Brusco, Janaína; Moreira, Jorge E.; Rasia‐Filho, Alberto A.

doi:10.1016/j.jneumeth.2010.03.018

Cited by 15 publications

(25 citation statements)

References 37 publications

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“…All the present methodological procedures were reported previously. Briefly, we used the “single‐section” Golgi method to characterize neuronal cell bodies and spiny dendrites according to Dall'Oglio, Ferme, Brusco, Moreira, and Rasia‐Filho (); Dall'Oglio et al (, ). Afterward, we used a new algorithm for the 3D reconstruction and precise visualization of pleomorphic dendritic spines described by Reberger, Dall´Oglio, Jung, and Rasia‐Filho ().…”

Section: Methodsmentioning

confidence: 99%

“…One series was sectioned at 50 μm for the thionin technique and another series was sectioned at 200 μm for the Golgi method. We used the same material processed previously and stored at a federal facility (UFCSPA, Brazil) by Dall'Oglio et al (, , ) and Reberger et al ().…”

Section: Methodsmentioning

confidence: 99%

“…The “single‐section” Golgi method (see further description in Dall'Oglio et al, , and references therein) was used to study the shape of CoA neurons in all cases (Table ). After additional 3 days immersed in the postfixation solution at RT, the sections were (a) rinsed in PBS and transferred to a solution of 0.1% osmium tetroxide (Sigma Chemicals Co.) in PBS for 20 min; (b) rinsed in PBS and immersed in 3% potassium dichromate (Merck) at 4 °C in the dark for 2 days; (c) rinsed again in distilled water, “sandwiched” between coverslips, and placed in a solution of 1.5% silver nitrate (Merck) at RT in the dark for 1 day; and (d) washed in distilled water.…”

Section: Methodsmentioning

confidence: 99%

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Neuronal types of the human cortical amygdaloid nucleus

Vásquez

Reberger

Dall'Oglio

et al. 2018

J of Comparative Neurology

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The human cortical amygdaloid nucleus (CoA) receives exteroceptive sensory stimuli, modulates the functions coded by the intrinsic amygdaloid circuit, and constitutes the beginning of the limbic lobe continuum with direct and indirect connections toward subcortical, allocortical, and higher order neocortical areas. To provide basic data on the human CoA, we characterized and classified the neurons using the thionin and the "single-section" Golgi method adapted for postmortem brain tissue and light microscopy. We found 10 different types of neurons named according to the morphological features of the cell body, dendritic branches, and spine distribution. Most cells are multipolar spiny neurons with two or more primary dendrites, including pyramidal-like ones. Three-dimensional reconstructions evidenced the types and diversity of the dendritic spines in each neuron. The unlike density of spines along dendritic branches, from proximal to distal ones, indicate that the synaptic processing and plasticity can be different in each CoA neuron. Our study provides novel data on the neuronal composition of the human CoA indicating that the variety of cells in this region can have phylogenetic, ontogenetic, morphological, and likely functional implications for the integrated human brain function. This can reflect both a more complex subcortical synaptic processing of sensory and emotional information and an adaptation for species-specific social behavior display.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Neuronal types of the human cortical amygdaloid nucleus

Vásquez

Reberger

Dall'Oglio

et al. 2018

J of Comparative Neurology

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“…() and Bolam (), and adapted for the human nervous tissue by Dall'Oglio et al. (, ). Briefly, bilateral brain blocks of the anterior temporal lobe (cases 1 to 3, Table ) were sectioned to approximately 1.5 cm 3 to contain the Me.…”

Section: Methodsmentioning

confidence: 99%

The human medial amygdala: structure, diversity, and complexity of dendritic spines

et al. 2015

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The medial nucleus of the amygdala (Me) is a component of the neural circuit for the interpretation of multimodal sensory stimuli and the elaboration of emotions and social behaviors in primates. We studied the presence, distribution, diverse shape, and connectivity of dendritic spines in the human Me of adult postmortem men. Data were obtained from the five types of multipolar neurons found in the Me using an adapted Golgi method and light microscopy, the carbocyanine DiI fluorescent dye and confocal microscopy, and transmission electron microscopy. Three-dimensional reconstruction of spines showed a continuum of shapes and sizes, with the spines either lying isolated or forming clusters. These dendritic spines were classified as stubby/wide, thin, mushroom-like, ramified or with an atypical morphology including intermediate shapes, double spines, and thorny excrescences. Pleomorphic spines were found from proximal to distal dendritic branches suggesting potential differences for synaptic processing, strength, and plasticity in the Me neurons. Furthermore, the human Me has large and thin spines with a gemmule appearance, spinules, and filopodium. The ultrastructural data showed dendritic spines forming monosynaptic or multisynaptic contacts at the spine head and neck, and with asymmetric or symmetric characteristics. Additional findings included en passant, reciprocal, and serial synapses in the Me. Complex long-necked thin spines were observed in this subcortical area. These new data reveal the diversity of the dendritic spines in the human Me likely involved with the integration and processing of local synaptic inputs and with functional implications in physiological and various neuropathological conditions.

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“…In addition to lack of uniform staining, consistency and reproducibility, this method is also time consuming, which practically limits its use as the first choice, particularly for the study of formalin-fixed brain tissue (Williams, 1983). It was also a mystery why sometimes glial cells were stained along with neurons which interfered analysis and interpretation (Adams, 1979;D'Amelio, 1983;Dall'Oglio et al, 2010;Howard and Barnes, 1979;Klatzo, 1952;Marani et al, 1987;Moliner, 1957;Zhang et al, 2003). Further, some authors commented that Golgi method (including its variants in general) worked better for neuronal staining with fresh brain tissue (Marin-Padilla, 1995) or tissue that was fixed in formalin for a very short duration, a few hours to few days (Marin-Padilla, 1995).…”

Section: Introductionmentioning

confidence: 99%