The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.
ObjectivesThis study was carried out to investigate the effect of rapid eye movement sleep (REMS) deprivation (REMSD) on the cytomorphology of the dorsal raphe (DR) neurons and to evaluate the possible role of REMSD-induced increased noradrenalin (NA) in mediating such effects.MethodsRats were REMS deprived by the flowerpot method; free moving normal home cage rats, large platform and post REMS-deprived recovered rats were used as controls. Further, to evaluate if the effects were induced by NA, separate sets of experimental rats were treated (i.p.) with α1-adrenoceptor antagonist, prazosin (PRZ). Histomorphometric analysis of DR neurons in stained brain sections were performed in experimental and control rats; neurons in inferior colliculus (IC) served as anatomical control.ResultsThe mean size of DR neurons was larger in REMSD group compared to controls, whereas, neurons in the recovered group of rats did not significantly differ than those in the control animals. Further, mean cell size in the post-REMSD PRZ-treated animals was comparable to those in the control groups. IC neurons were not affected by REMSD.ConclusionsREMS loss has been reported to impair several physiological, behavioral and cellular processes. The mean size of the DR neurons was larger in the REMS deprived group of rats than those in the control groups; however, in the REMS deprived and prazosin treated rats the size was comparable to the normal rats. These results showed that REMSD induced increase in DR neuronal size was mediated by NA acting on α1-adrenoceptor. The findings suggest that the sizes of DR neurons are sensitive to REMSD, which if not compensated could lead to neurodegeneration and associated disorders including memory loss and Alzheimer's disease.
Microgravity and sleep loss lead to cognitive and learning deficits. These behavioral alterations are likely to be associated with cytomorphological changes and loss of neurons. To understand the phenomenon, we exposed rats (225–275 g) to 14 days simulated microgravity (SMg) and compared its effects on CA1 hippocampal neuronal plasticity, with that of normal cage control rats. We observed that the mean area, perimeter, synaptic cleft, and length of active zone of CA1 hippocampal neurons significantly decreased while dendritic arborization and number of spines significantly increased in SMg group as compared with controls. The mean thickness of the postsynaptic density and total dendritic length remained unaltered. The changes may be a compensatory effect induced by exposure to microgravity; however, the effects may be transient or permanent, which need further study. These findings may be useful for designing effective prevention for those, including the astronauts, exposed to microgravity. Further, subject to confirmation, we propose that SMg exposure might be useful for recovery of stroke patients.
A prospective observational study was conducted at Bhimavaram hospitals Bhimavaram, to assess the symptoms, risk factors and prescribing patterns in cerebral stroke patients. A total of 80 patients of age group 20-90 (mean age 63.1) were considered in the study which includes both the genders. These patients were assessed regarding their demographics, symptoms, risk factors and prescribing patterns. The incidence of stroke was higher in males (70%) compared to females (30%). Out of these study population 85% experienced Ischemic stroke, 11% Transient ischemic attack (TIA) and 4% Hemorrhagic stroke. These patients presented weakness as common symptom, Hypertension as prominent risk factor and anti-platelet agents as commonly prescribed drugs. Early management of Hypertension and Diabetes may reduce the risk of stroke. Our study suggests that life style changes play a key role in prevention of stroke.
Rape is a form of sexual violence against women perpetrated by individuals, community groups or personnel of state agencies and others to establish their power. All rapes cannot be bracketed into a single category because the identity of the rapist and the one who has been raped may vary. Also, while some rapes elicit strong protests, there are other cases which are tacitly supported by the dominant majority. Rapes in regions of India which come under the Armed Forces (Special Powers) Act (AFSPA) fall into the latter category. Most of such rapes are inflicted with impunity by the members of the armed forces because of the protective provisions included in AFSPA. Such incidents never attract widespread anger because the victims are often from minority ethnic or religious groups. The irony is that the majority of people who participated in protests after Nirbhaya incident in Delhi may not even be aware of the numbers of such type of cases that have occurred in the North-East or in the Kashmir Valley.
Both the Bangladesh state and society are yet to settle the questions over and narratives related to the Liberation War of 1971. Broadly, there are two groups with contradictory and conflicting interpretations of the events related to that war. This has also led to the mushrooming of militant groups in the country. The beginning of trial of perpetrators of Liberation War crimes since 2010 and the execution of a few of the leaders has further polarised the society and politics of Bangladesh. The existing debates over the Bangladesh Liberation War cannot be studied without looking into the roles of India and Pakistan. The two countries have their own interpretations and political fallout of the 1971 liberation war.
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