Plasma homoarginine, arginine, asymmetric dimethylarginine and total homocysteine interrelationships in rheumatoid arthritis, coronary artery disease and peripheral artery occlusion disease

Kayaçelebi, Arslan Arinç; Willers, Janina; Pham, Vu Vi; Hahn, Andreas; Schneider, J.; Rothmann, Sabine; Frölich, Jürgen C.; Tsikas, Dimitrios

doi:10.1007/s00726-015-1915-3

Cited by 22 publications

(15 citation statements)

References 51 publications

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“…For North America and Australia, the rate is 8%-20%. The TT rate in Japan is 12% (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)24). The results of this study are in compliance with the frequency reported in white Turkish population.…”

Section: Discussionsupporting

confidence: 86%

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Investigation of the Methylenetetrahydrofolate Reductase Gene C677T and A1298G Polymorphism in Chronic Periodontitis

Kocaman¹

2019

Erciyes Med J

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Investigation of the Methylenetetrahydrofolate Reductase Gene C677T and A1298G Polymorphism in Chronic Periodontitis Objective: This case control study examined the frequency and distribution of the C677T and A1298C single-nucleotide polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene in Turkish patients with chronic periodontitis. Materials and Methods: This study included 89 subjects: 49 patients with chronic periodontitis and 40 healthy persons. Medical and periodontal histories were taken, and PD, CAL, PI, and BOP were recorded as the clinical parameters. The blood samples were taken from the subjects, and their DNA was obtained. The MTHFR gene's single nucleotide polymorphisms 677C→T and 1298A→C genotyping were analyzed using the RFLP assay, then PCR. Results: There was a significant difference between the groups except for age when the clinical findings. Single-nucleotide polymorphisms in the MTHFR gene C677T and 1298C regions were not found to be significantly different between the groups. Conclusion: The study revealed no association between the MTHFR polymorphism and the risk of chronic periodontitis; however, increasing the number of patients is useful when performing further studies. This present study is important because it is, to the best of our knowledge, the first study to research the interrelation between the MTHFR gene and chronic periodontitis.

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Section: Discussionsupporting

confidence: 86%

“…In severe MTHFR deficiency, hyperhomocysteinemia and homocysteinuria occur (12). Hyperhomocysteinemia is a risk factor for coronary heart, peripheral, vascular, and cerebrovascular disease (10)(11)(12)(13)(14). Recent studies reported an increased plasma Hcy levels in patients with chronic periodontitis (15).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Methylenetetrahydrofolate Reductase Gene C677T and A1298G Polymorphism in Chronic Periodontitis

Kocaman¹

2019

Erciyes Med J

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“…The results of our study confirm the findings of previous studies, which report an elevation or a significant increase in plasma Hcy concentration. Many studies have also shown that the level of Hcy is significantly elevated in patients with RA compared with the level of Hcy in healthy subjects (14)(15)(16).…”

Section: Discussionmentioning

confidence: 99%

Plasma Homocysteine Concentrations In Patients With Rheumatoid Arthritis

Vasiljevic

Lucic

Živanović³

et al. 2015

Serbian Journal of Experimental and Clinical Research

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In this study, we investigated the concentration of serum homocysteine (Hcy) in patients with rheumatoid arthritis (RA) compared with the control group and the connection between homocysteine and parameters of inflammation and disease activity. Sixty RA patients and 20 healthy controls were included in the study, and clinical examination and investigation were performed during which disease activity was assessed. Peripheral blood samples were used for all of the assays. Levels of Hcy were 33% higher in the RA patients than in the control subjects (mean +/− SD 11.79±3.72 μmol/L versus 8.90±1.38 μmol/L; p< 0.01). A significant correlation was found between parameters of inflammation (C-reactive protein) and homocysteine in patients (r=0.322, p=0.012). Patients with high disease activity had a significantly greater increase in homocysteine (p<0.05). An increase in plasma homocysteine in RA patients is related to the parameters of inflammation and disease activity. Elevated Hcy levels occur commonly in patients with RA and may explain some of the increased cardiovascular mortality seen in RA patients.

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“…In RA, this could be beneficial since elevated plasma homocysteine has been associated with increased risk of cardiovascular diseases. 27 …”

Section: Discussionmentioning

confidence: 99%

MicroRNAs interfere with DNA methylation in rheumatoid arthritis synovial fibroblasts

Gaur¹,

Karouzakis²,

Glück³

et al. 2016

RMD Open

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BackgroundThe DNA of rheumatoid arthritis synovial fibroblasts (RASF) is globally hypomethylated; this contributes to an aggressive behaviour. In an attempt to remethylate these cells, we supplemented with methyl donors. We investigated the possible interference of microRNAs (miRs).Material and methodsRASF were treated with L-methionine or betaine. Transcripts of de novo methyltransferases (DNMTs) and miRs were measured by real-time PCR, and a transcription PCR array was performed. Levels of homocysteine, matrix metalloproteinase-1 (MMP-1) and global DNA methylation were determined. Transfection with lipofectamine was performed with specific pre-miRs and anti-miRs, such as miR29 and let7f.ResultsL-methionine was more efficient to increase DNA methylation than betaine. This was associated with a reduced expression of DNMT3A mRNA in betaine-treated RASF. Betaine increases the expression of miR29 in RASF which targets DNMT3A, thereby limiting the remethylation process. Nevertheless, betaine inhibited the expression of multiple transcription factors, decreased the release of MMP-1, biosynthesis of homocysteine and cell migration.ConclusionAlterations in cellular miRs profiles, in particular the upregulation of miR29, which targets DNMT3A, may limit the efficiency of betaine if it is used as DNA remethylating agent. However, L-methionine also has similar impact on miR29 expression. On the other hand, betaine has multiple other beneficial effects on the activated phenotype of RASF; it is not excluded that the effect of betaine on DNMT3A is, at least in part, indirect. Clinical trials with betaine could be promising.

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Plasma homoarginine, arginine, asymmetric dimethylarginine and total homocysteine interrelationships in rheumatoid arthritis, coronary artery disease and peripheral artery occlusion disease

Cited by 22 publications

References 51 publications

Investigation of the Methylenetetrahydrofolate Reductase Gene C677T and A1298G Polymorphism in Chronic Periodontitis

Investigation of the Methylenetetrahydrofolate Reductase Gene C677T and A1298G Polymorphism in Chronic Periodontitis

Plasma Homocysteine Concentrations In Patients With Rheumatoid Arthritis

MicroRNAs interfere with DNA methylation in rheumatoid arthritis synovial fibroblasts

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