Plaque Formation by Mumps Virus and Inhibition by Antiserum

Flanagan, Thomas D.; Barron, Almen L.

doi:10.1128/aem.19.2.360-366.1970

Cited by 13 publications

(7 citation statements)

References 6 publications

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“…HAI and neutralization tests showed the presence of antibody in serum samples. The magnitude of response was significant and similar in most cases to human cases of natural mumps (7). The lowest antibody response observed was that occurring in the animal which received a 25-mg dose of cortisone acetate.…”

Section: Contaminatedmentioning

confidence: 56%

“…This agent was passaged nine times in primary human amnion cell cultures and two or three times in primary rhesus thyroid cell cultures. The preparations used to inoculate animals titered 4 X 107 plaque-forming units (PFU)/ml (7). Virus diluent was medium 199 with 2% newborn calf serum.…”

Section: Methodsmentioning

confidence: 99%

“…Neutralization titers were expressed as the reciprocal of serum dilution which resulted in 80% plaque reduction. These procedures have been described (7) in another communication.…”

Section: Methodsmentioning

confidence: 99%

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Response to Experimental Infection with Mumps Virus in Rhesus Monkeys

et al. 1971

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Experiments were conducted in which rhesus monkeys were infected with mumps virus by inoculation into the parotid gland. Virus was isolated from buccal swab specimens from animals up to 6 days after inoculation. Immunofluorescence staining for mumps antigen was positive, and infectious virus was recovered from tissue biopsies of inoculated glands. Histological examination of biopsied glands revealed lymphocytic infiltration of the tissue. Virus was not isolated from urine, buffy coat, or biopsies of thyroid and spleen. In other experiments, animals were infected by inoculation into the thyroid gland. Virus was not isolated from buccal swabs, urine specimens, or thyroid tissues from these monkeys. All inoculated animals responded immunologically to mumps virus regardless of route of infection. Previously infected animals did not shed virus when challenged with mumps virus. A histological response to challenge in glandular tissue was observed which suggested a hypersensitivity to mumps antigen. The response was qualitatively identical to that in acutely infected tissue but more intense. None of the animals infected developed antibodies to thyroid tissue regardless of route of injection of virus or site of challenge of immune monkeys.

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Section: Contaminatedmentioning

confidence: 56%

Section: Methodsmentioning

confidence: 99%

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Response to Experimental Infection with Mumps Virus in Rhesus Monkeys

et al. 1971

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“…Infectivity assays. Infectious measles virus and mumps virus were assayed by a plaque formation method in BGM cells as previously described for mumps virus (3). VSV was also titered by plaque assay.…”

Section: Methodsmentioning

confidence: 99%

Characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the BGM/MV cell line

Menna¹,

Collins²,

Flanagan³

1975

Infect Immun

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Serological methods of mixed agglutination and indirect immunofluorescence showed the BGM/MV cell line to possess only monkey antigens. As a means of further characterizing the species constitution of the BGM/MV cell line, the species specificity of viral-induced interferon from these cells, as well as the response of these cells to exogenous interferons, was determined. Low titers of spontaneously elaborated interferon capable of protecting monkey but not mouse cells were detected in BGM/MV culture fluids. Interferon induced by Newcastle disease virus infection of BGM/MV cells was capable of conferring an antiviral state on monkey and, to a lesser extent, on mouse cells. Exogenous interferons of both homologous (BGM/MV) and heterologous sources failed to confer an antiviral state on BGM/MV cells. BGM/MV cells were found to be partially refractive to superinfection with measles virus but freely replicated mumps and vesicular stomatitis virus.

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“…Virus antibody. Antibody to mumps virus was determined by a plaque reduction neutralization test (7). The titers were expressed as the reciprocal of the dilution of serum or parotid extract which resulted in a 50O reduction of input virus.…”

Section: Methodsmentioning

confidence: 99%

Immunocyte Response to Experimental Mumps Virus Infection in Rhesus Monkeys

1973

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Nineteen rhesus monkeys were inoculated with mumps virus by retrograde ductal inistillation into the parotid gland. Evidence of infectionl was obtained in all instainces. Virus was isolated from buccal swabbings and parotid biopsies for 1 week after inoculation. A vigorous serum-neutralizing antibody response occurred within 3 weeks, and there was marked monocytic infiltration of the parotid stroma. The monocytic infiltrate comprised as much as 60% of the total gland volume 1 week after infection. The predominant inflammatory cells were non-immunoglobulincontaining mononuclear cells resembling lymphocytes. Plasma cells containing immunoglobulin G (IgG), IgA, IgM, and IgE increased in numbers in the gland after infection, the greatest increase occurring in IgG-containing cells. Neutralizing antibodies and inlterferon were found in extracts prepared from the infected glands. Neutralizing activity was highest in samples taken 3 weeks after infection but was detectable in samples taken as soonl as 36 to 48 h after infection. Interferon activity was detected in signiificaint amounts 36 to 48 h after infection. Challenge of previously inifected ainimals resulted in an increase ill the monocytic infiltrate as well as an increase in numbers of immunoglobulini-containiiig plasma cells. However, reinfection did not occur as evidenced by the inability to culture shed virus after challenge. This model should be useful for in vivo study of biochemical mediators which evoke inflammatory cell infiltration and which may be significant both in protection and in tissue damage.

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Plaque Formation by Mumps Virus and Inhibition by Antiserum

Cited by 13 publications

References 6 publications

Response to Experimental Infection with Mumps Virus in Rhesus Monkeys

Response to Experimental Infection with Mumps Virus in Rhesus Monkeys

Characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the BGM/MV cell line

Immunocyte Response to Experimental Mumps Virus Infection in Rhesus Monkeys

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