Characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the BGM/MV cell line

Menna, Jay H.; Collins, Arlene R.; Flanagan, Thomas D.

doi:10.1128/iai.11.1.159-163.1975

Cited by 10 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This paper and the accompanying paper (6) describe a regulated type of persistent-state measles virus infection of a cell line established in our laboratory. The evolution of this cell line is described with respect to cellular morphology and state of virus infection.…”

mentioning

confidence: 99%

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line

Menna¹,

Collins²,

Flanagan³

1975

Infect Immun

Self Cite

View full text Add to dashboard Cite

The parameters of a persistent-state measles virus infection in BGM/MV cells were examined. The BGM/MV cell line was established by cocultivation of measles virus-infected primary C3H mouse brain cells with a stable line of African green monkey kidney cells (BGM). Initially, a morphologically mixed population of cells existed: BGM-like (epithelioid) and fibroblasts. Gradually the fibroblasts were replaced by BGM-like cells, resulting in a morphologically homogeneous population. Measles cytopathic effect was noted 2 days after initiation of this culture and persisted for approximately 290 days. The time of disappearance of viral cytopathic effect corresponded to the time at which morphological homogeneity was reached. Low titers of infectious measles virus were detected in the BGM/MV culture up to 20 days postseeding; thereafter none was observed. After 440 days in culture, 100% of BGM/MV cells demonstrated intractyoplasmic measles antigen by immunofluorescence. Nuclear fluorescence was never observed. Electron microscopy revealed the presence of measles virus nucleocapsid within and almost completely filling the cytoplasm of BGM/MV cells. The plasma membrane of these cells appeared normal; no maturing or budding particles were observed. Measles virus hemagglutinin was not detected in either clarified cell lysates or in supernatant culture fluids. Cell membrane alteration by measles virus was detected in less than 1% of these cells by hemadsorption and by membrane immunofluorescence. The hemadsorption activity of the cells could be enhanced (30 to 70%) by treatment with actinomycin D or enucleation with cytochalasin B; these treatments, however, were unsuccessful in inducing detectable levels of measles hemagglutinin. Treatment of BGM/MV cells with 5-bromo-2'-deoxyuridine (BUdR) at 5 to 50 Ag/ml and cytosine arabinoside at 1 to 50 gg/ml failed to enhance hemadsorption activity. Doses of 5bromo-2'-deoxyuridine ranging from 5 to 200 ,ug/ml and of actinomycin D ranging from 0.1 to 10 Ag/ml were ineffective in inducing the synthesis of infectious virus. Various physical methods of induction of infectious virus was also unsuccessful.

show abstract

mentioning

confidence: 99%

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line

Menna¹,

Collins²,

Flanagan³

1975

Infect Immun

Self Cite

View full text Add to dashboard Cite

show abstract

Enhanced parainfluenza I (6/94) virus detection in latently infected human brain cell cultures by treatment with cytochalasin D and dimethyl sulfoxide

Wróblewska

Wellish

Gilden

1978

Journal of Medical Virology

View full text Add to dashboard Cite

The ability of cytochalasin D (CD) and dimethyl sulfoxide (DMSO) to enhance parainfluenza I (6/94) virus replication was studied in various cell culture systems. Treatment of CV1 cells with CD (1 microgram/ml) dissolved in DMSO prior to primary 6/94 virus exposure at 10(0)--10(5) multiplicities of infection did not substantially enhance virus replication. However, there was a transient increase in cell associated virus one day after infection of DMSO-treated cultures. CD treatment of cultures of human brain cells latently infected with 6/94 virus (LIHB cells) did not enhance 6/94 virus detection. Cocultivation of CV1 cells with CD-treated LIHB cell cultures, and cocultivation of LIHB cell cultures with CD-treated CV1 cells, resulted in the production of both cell-associated and cell-free 6/94 virus three and five days after cocultivation. No virus was detected after similar cocultivation of untreated LIHB cell cultures with untreated CV1 cells. The usefulness of CD-DMSO treatment in the rescue of virus from 6/94 LIHB cell cultures appears limited to a cocultivation system. The use of these techniques to enhance virus rescue from human tissues suspected of harboring latent viral genomes is discussed.

show abstract

Interferon production and response to exogenous interferon in two cell lines of mouse brain origin persistently infected with Sendai virus

Collins

Flanagan

1977

Archives of Virology

View full text Add to dashboard Cite

Two persistently infected cell lines established from C3H mouse brain cells infected in vivo with Sendai virus were shown to differ with respect to interferon (IF) production and response to exogenous IF. MB/Sen carrier cells contained 1-5 per cent antigen positive cells when examined by immunofluorescence, and virus was occasionally recovered from the culture medium. MB/SenAS carrier cells were maintained with 0.16 per cent Sendai antiserum in the supernatant medium. All MB/SenAS cells contained viral antigen and infectious virus was present in the culture medium. MB/Sen released IF spontaneously into the culture medium. Further IF production could be stimulated in MB/Sen by superinfection with Newcastle disease virus (NDV) or vesicular stomatitis virus (VSV). Exogenous IF provided good protection against VSV challenge. In contrast, MB/SenAS produced no IF spontaneously but could be stimulated by NDV and VSV to produce IF. Exogenous IF failed to reduce the amount of VSV released into the supernatant fluid. Replication of VSV was restricted in MB/SenAS as shown by a 2.3 log10 lower virus yield compared to MB/Sen.

show abstract

Characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the BGM/MV cell line

Cited by 10 publications

References 9 publications

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line

Enhanced parainfluenza I (6/94) virus detection in latently infected human brain cell cultures by treatment with cytochalasin D and dimethyl sulfoxide

Interferon production and response to exogenous interferon in two cell lines of mouse brain origin persistently infected with Sendai virus

Contact Info

Product

Resources

About