Experimental orchitis was first produced by Voisin et al. (1951) by iso-and autosensitization in guinea pigs ( 1 ) , by Freund et al. (1954) in rats (2), and by Pokorna et d. (1963) in mice (3). Recently, Mancini, Andrada et d. (1965) were able to produce allergic orchitis in man (4). A group of four volunteer patients suffering from prostatic carcinoma was studied. Each of the four patients was immunized prior to surgical castration with testicular suspensions (autoimmunization and isoimmunization) . After a period of 3-8 weeks, two of the four patients developed patchy lesions in the contralateral testis consisting of congestion, serous edema and sloughing of germinal cells. Low titered circulating antibodies also appeared. To obtain a model for autoallergic orchitis closely related to man, an attempt was made to induce autoallergic orchitis in monkeys.
Materials and Methods. Animals.Eight adult male rhesus monkeys (Macaca mulat t a ) 5 years of age or older and weighing 15-20 lb were selected for these experiments. They were unilaterally orchiectomized (hemicastrated) and blood specimens were obtained. The histological picture of the testis was normal from all animals except one (Monkey no. 4) which presented arrest of spermatogenesis in about 50% of the tubules.Tissue extracts. The testicular material obtained from each experimental animal at the time of hemicastration, if not used immediately, was preserved at -40'. The glands were trimmed, washed in cold buffered saline, cut up and homogenized for 5 min in phosphate buffered saline, pH 7.2, by means of a Teflon homogenizer. Two ml of saline was
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