Plants used by the Cree Nation of Eeyou Istchee (Quebec, Canada) for the treatment of diabetes: A novel approach in quantitative ethnobotany

Leduc, Charles; Coonishish, Jason; Haddad, Pierre S.; Cuerrier, Alain

doi:10.1016/j.jep.2005.09.038

Cited by 129 publications

(120 citation statements)

References 21 publications

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“…(American cranberry) are recognized as sources of antidiabetic phytochemicals, recent surveys have identified V. angustofolium Ait. (Canadian lowbush blueberry) as highly recommended by Quebec traditional practitioners and Cree Elders of Eeyou Istchee for treatment of diabetic symptoms and complications (Haddad et al, 2003;Leduc et al, 2006). Lowbush blueberry fruits are collected and consumed throughout regions of north-eastern North America and possess well-documented antioxidant properties (Costantino et al, 1992;Kalt et al, 1999;Wang and Jiao, 2000;Kay and Holub, 2002;Rimando et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

Harris

Burt

Saleem

et al. 2007

Phytochemical Analysis

107

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A method was developed for the analysis of Vaccinium angustifolium Ait. (Lowbush blueberry), which is a widely used natural health product, particularly for the treatment of diabetic symptoms. While the anthocyanin content of the fruit has been well characterized, the chemistry of the vegetative parts used in supportive therapy for diabetes has been largely ignored. Using a metabolomics-based approach for compound identification with an emphasis on phenolic metabolites, a single HPLC-PAD-APCI/ MS method was developed for the separation and quantitation of the major metabolites found in the 95% ethanol extracts of leaf, stem, root and fruit. The leaf extract contained high concentrations of chlorogenic acid (~100 µg/mg extract) and a variety of quercetin glycosides that were also detected in the fruit and stem extracts. Flavan-3-ol monomers (+)-catechin and (−)-epicatechin were found in all plant parts but their procyanidin dimers were exclusively identified in the stem and root. The accuracy and precision of the presented method were corroborated by low intra-and inter-day variations in quantitative results in all plant part extracts. Further validation of the extraction and analytical protocols focused on identified compounds with reputed anti-diabetic activity, revealing recoveries greater than 80% and detection limits of 0.12-2.73 µg/mL.

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Section: Introductionmentioning

confidence: 99%

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

Harris

Burt

Saleem

et al. 2007

Phytochemical Analysis

107

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“…When the bioactivities of extracts made from separated organs of the same species (i.e., root, stem, leaf, and fruit extracts) were compared, certain plants exhibited organ-specific activities , some of which could be attributed to differences in phenolic constituents within the plant organs (Harris et al, 2007). Here, we extend these findings to an analysis of minhikw, the Cree term for Picea glauca, a plant reported useful for controlling diverse diabetic complications among several First Nations groups (Arnason et al, 1981;Leduc et al, 2006), particularly the Cree of Whapmagoostui. We report that polar fractions of Picea glauca (minhikw) needle are more effective than bark and cone extracts at protecting PC12 peripheral neuronal precursors from both glucose toxicity and glucose deprivation.…”

Section: Introductionmentioning

confidence: 64%

Antidiabetic Activity of Extracts from Needle, Bark, and Cone ofPicea glauca.: Organ-Specific Protection from Glucose Toxicity and Glucose Deprivation

Harris¹,

Lambert²,

Saleem³

et al. 2008

Pharmaceutical Biology

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The incidence of type 2 diabetes mellitus has reached epidemic proportions worldwide. Canadian aboriginal communities, particularly the Cree Nation of Eeyou Istchee, have been identified as a high-risk population. Culturally acceptable treatment options are limited notably for diabetic complications resulting in peripheral neuropathy. Here, we describe results of an ongoing collaborative research project with Cree of Eeyou Istchee to identify botanicals capable of protecting peripheral neuronal precursors from glucose toxicity and glucose deprivation in vitro. Polar fractions of three plant organs (needles, cone, and bark) collected from Picea glauca (Moench) Voss (Pinaceae), were tested for toxicity under normoglucose, glucotoxicity, and glucose deprivation conditions. The profile of phenolic metabolites in each extract was first characterized by high-performance liquid chromatography-diode array detection-atmospheric pressure chemical ionisation mass spectrometry (HPLC-DAD-APCI/MS). We report here that these fractions are well-tolerated by PC12 neuronal precursors under normoglucose conditions. LD 50 concentrations of needle extracts exceeded 100 µg/mL, whereas the LD 50 of bark and cone extracts was 40 and 36.4 µg/mL, respectively. We further show that the cytoprotective properties of minhikw after glucose challenge are concentration-dependent and organ-specific. Needle * Dedicated to John Thor Arnason of the University of Ottawa, Department of Biology, on the occasion of his sixtieth birthday. extracts protected PC12 cells from both glucotoxicity and glucose deprivation. Bark extracts had negligible activity. Cone extracts further impaired PC12 cell glucose tolerance. This study provides the first validation of antidiabetic activity of minhikw organs at the cellular level relevant to the management of diabetic peripheral neuropathy.

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“…Dessa forma, os pesquisadores selecionaram 16 espécies para teste de atividade antibacteriana, sendo que 75% destas mostraram a referida atividade. Técnicas quantitativas também têm sido usadas para selecionar plantas com atividade hipoglicemiante (Leduc et al, 2006;AndradeCetto et al, 2006;Funke;Melzig, 2006). Utilizando a lógica do consenso dos informantes, Berlin (1999) analisou a variação léxica nos nomes atribuídos as plantas medicinais entre 14 municípios Tzotzil e Tzeltal, no México.…”

Section: Problemas Na Apresentação E Análise Dos Dadosunclassified

As pesquisas etnodirigidas na descoberta de novos fármacos de interesse médico e farmacêutico: fragilidades e pespectivas

Albuquerque¹,

Hanazaki²

2006

Rev. bras. farmacogn.

155

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RESUMO:As investigações etnofarmacológicas e etnobotânicas têm sido a principal abordagem reconhecida por cientistas em todo o mundo, como uma estratégia de seleção de plantas medicinais.As qualidades e fortalezas dessas abordagens já foram sufi cientemente discutidas, restando poucas dúvidas de seu potencial e impactos biológicos, econômicos e sociais. Este artigo focaliza os estudos etnodirigidos (etnobotânicos e etnofarmacológicos) levantando os problemas encontrados na qualidade das pesquisas, que podem afetar a sua aplicação na descoberta de novos fármacos de interesse médico e farmacêutico. Adicionalmente, apontamos alguns elementos que podem colaborar para a melhoria da qualidade das abordagens e publicações e que podem ser aplicados a diferentes estudos etnodirigidos. Nós também sugerimos que os investigadores reconsiderem algumas das abordagens dominantes, bem como a visão, não raro simplista, desses estudos.Unitermos: Etnobotânica, etnofarmacologia, métodos em etnobotânica, método científi co, plantas medicinais, bioprospecção.ABSTRACT: "Ethnodirected research in the discovery of new drugs of medical and pharmaceutical interest: fl aws and perspectives". Ethnopharmacological and ethnobotanical investigations have been the main approach used by scientists all over the world as a strategy to select medicinal plants. The qualities and strengths of these approaches have been suffi ciently discussed, and there are a few doubts left in relation to their potential and biological, economic, and social impacts. This article focuses on ethnodirected studies (ethnopharmacological and ethnobotanical), raising problems found in the quality of these studies. In addition, we point out some elements that might contribute to the improvement of approaches and publications that can be applied to different ethnodirected studies. We also suggest that researchers reconsider some of the dominant approaches, as well as the view of these studies, which is often simplistic.. Keywords:Ethnobotany, ethnopharmacology, methods in ethnobotany, scientifi c methods, medicinal plants, bioprospecting. INTRODUÇÃOMuitas áreas estão envolvidas na pesquisa de novas substâncias oriundas de plantas, como a fi toquímica, que trabalha no isolamento, purifi cação e caracterização de princípios ativos; a etnobotânica e a etnofarmacologia, que buscam informações a partir do conhecimento de diferentes povos e etnias; e a farmacologia, que estuda os efeitos farmacológicos de extratos e dos constituintes químicos isolados (Maciel et al., 2002;Mendonça-Filho;Menezes, 2003;Vendruscolo et al., 2005). Nos últimos anos, no Brasil, várias pesquisas foram realizadas com contribuições relevantes sobre o assunto, não só quanto a aspectos fi toquímicos, mas também quanto à atividade biológica de plantas que ocorrem nos diferentes ecossistemas brasileiros (Desmarchelier et al., 1999;Jorge et al., 2004;Duarte et al., 2004;Lima et al., 2006;Hiruma-Lima et al., 2006).Há, nesse sentido, vários caminhos para o estudo de plantas medicinais, destacando-se quatro tipos...

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Plants used by the Cree Nation of Eeyou Istchee (Quebec, Canada) for the treatment of diabetes: A novel approach in quantitative ethnobotany

Cited by 129 publications

References 21 publications

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

Antidiabetic Activity of Extracts from Needle, Bark, and Cone ofPicea glauca.: Organ-Specific Protection from Glucose Toxicity and Glucose Deprivation

As pesquisas etnodirigidas na descoberta de novos fármacos de interesse médico e farmacêutico: fragilidades e pespectivas

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