Plant regeneration through somatic embryogenesis from leaf and root explants of<i>Rhynchostylis retusa</i>(L.) Blume

Islam, S. M. Shahinul; Bhattacharjee, Bakul

doi:10.5958/0974-4517.2015.00025.7

Cited by 10 publications

(6 citation statements)

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“…In recent years, plant regeneration via direct somatic embryogenesis had been successfully established in various orchids including Dendrobium (Chung et al 2005), Epipactis (Moradi et al 2017), Malaxis (Mahendran and Bai 2016), Phalaenopsis (Chen and Chang 2006; Gow et al 2010; Feng and Chen 2014), Rhynchostylis (Islam and Bhattacharjee 2015) and also a Tolumnia related genus, Oncidium (Chen et al 1999; Chen and Chang 2002; Su et al 2006; Hong et al 2008a, b). In these previous studies, cytokinins, especially thidiazuron, were found to be effective to induce direct somatic embryogenesis from the leaf explants (Chen et al 1999; Chen and Chang 2002; Su et al 2006; Hong et al 2008a, b).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

et al. 2018

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BackgroundTolumnia genus (equitant Oncidium) is a group of small orchids with vivid flower color. Thousands of hybrids have been registered on Royal Horticulture Society and showed great potential for ornamental plant market. The aim of this study is to establish an efficient method for in vitro propagation.ResultsLeaf explants taken from in vitro-grown plants were used to induce direct somatic embryogenesis on a modified 1/2 MS medium supplemented with five kinds of cytokinins, 2iP, BA, kinetin, TDZ and zeatin at 0.3, 1 and 3 mg l−1 in darkness. TDZ at 3 mg l−1 gave the highest percentage of explants with somatic globular embryos after 90 days of culture. It was found that 2,4-D and light regime highly retarded direct somatic embryogenesis and showed 95–100% of explant browning. Histological observations revealed that the leaf cells divided into meristematic cells firstly, followed by somatic proembryos, and then somatic globular embryos. Eventually, somatic embryos developed a bipolar structure with the shoot apical meristem and the root meristem. Scanning electron microscopy observations showed that the direct somatic embryogenesis from leaf explants was asynchronously. The somatic embryos were found on the leaf tip, the adaxial surface and also the mesophyll through a cleft, and it reflected the heterogeneity of the explant. The 90-day-old globular embryos were detached from the parent explants and transferred onto a hormone-free 1/2 MS medium in light condition for about 1 month to obtain 1-cm-height plantlets. After another 3 months for growth, the plantlets were potted with Sphagnum moss and were acclimatized in a shaded greenhouse. After 1 month of culture, the survival rate was 100%.ConclusionsIn this report, a protocol for efficient regenerating a Tolumnia orchid, Louise Elmore ‘Elsa’, was established via direct somatic embryogenesis and might reveal an alternative approach for mass propagation of Tolumnia genus in orchid industry.

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Section: Introductionmentioning

confidence: 99%

Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

et al. 2018

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“…Sagarik) được vi nhân giống bằng mô sẹo phôi hóa [9]. Tái sinh cây thông qua hình thành phôi soma từ mảnh cắt lá và rễ của lan Đuôi chồn Rhynchostylis retusa (L.) Blume [10]. Ảnh hưởng của các chất điều hòa sinh trưởng lên sự phát triển, sự tái sinh và nhân sinh khối protocorm có nguồn gốc từ hạt non của lan Đuôi chồn (R.retusa) đã được báo cáo [11].…”

Section: đặT Vấn đềunclassified

“…Tuy nhiên, chiều dài rễ thu được cao hơn (3,3 cm), rễ mập, xanh, có nhiều lông tơ [12]. Theo Islam và Bhattacharjee (2015), khi nghiên cứu khả năng ra rễ của chồi Rhynchostylis retusa (L.) Blume đã thu được kết quả môi trường ½MS + IAA 0,5 mg/l và IBA 0,5 mg/l cho số lượng rễ cao nhất (5,8) và chiều dài rễ cao nhất (4,17 cm) [10].…”

Section: ảNh Hưởng Của Naa đếN Khả Năng Ra Rễ Của Chồiunclassified

Vi Nhân Giống Lan Đai Châu (Rhynchostylis Gigantea L.) Thông Qua Giai Đoạn Protocorm

Thanh¹,

Lan²

2021

TNUJST

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Lan Đai châu là loài lan bản địa quý của Việt Nam có nguy cơ tuyệt chủng do mất môi trường sống thích hợp và sự khai thác quá mức của con người. Quy trình vi nhân thông qua phát sinh protocorm từ mô sẹo đã được xây dựng thành công để bảo tồn và phát triển nguồn gen loài lan này. Môi trường nuôi cấy bổ sung các chất điều hòa sinh trưởng BAP, NAA và dịch chiết khoai tây, chuối tiêu đến sự tạo protocorm, nhân sinh khối và tái sinh chồi đã được khảo sát. Môi trường MS bổ sung BAP 2,0 mg/l phù hợp cho tạo protocorm từ mô sẹo, tỉ lệ đạt 100%. Môi trường nhân protocorm phù hợp nhất là môi trường VW bổ sung BAP 1,0 mg/l, NAA 0,5 mg/l, nước dừa 100 ml/l, khoai tây 40 g/l, chuối tiêu 30 g/l cho sinh khối protocorm tăng 4,66 lần so với ban đầu. Môi trường VW bổ sung nước dừa 100 ml/l, khoai tây 40 g/l, chuối tiêu 30 g/l cho hiệu quả nhân sinh khối PLBs tăng 17,9 lần so với ban đầu và tái sinh chồi tốt nhất. Môi trường VW bổ sung NAA 1,0 mg/l, chuối tiêu 50 g/l phù hợp nhất cho ra rễ của chồi với số rễ đạt 4,0 rễ/chồi, chiều dài rễ đạt 2,33 cm. Quy trình vi nhân giống này có thể ứng dụng để sản xuất cây giống Đai châu với quy mô lớn.

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“…By using direct regeneration method, shoots and roots could be developed directly from an explant (Ayob et al 2013;Raju et al 2015). Leaf and cotyledon are one of the most desirable explants that are commonly used for in vitro regeneration due to their ability to preserve the genetic homozygosity (Huang et al 2014;Islam and Bhattacharjee 2015).…”

Section: Introductionmentioning

confidence: 99%

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

Alttaher¹

2021

IJAB

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Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate and efficiency. This has made an in vitro propagation of E. longifolia a desirable alternative. Hence, this study reports an effective method of direct organogenesis of shoot. In vitro seedling’s leaves were cultured on Murashige and Skoog (MS) medium containing 1.0 mg L-1 6-benzylaminopurine (BAP), producing 1.8 ± 0.5 shoots per leaf with a regeneration frequency of 68.2%. The shoot buds were directly formed from leaves without intermediate callus formation. To obtain complete plantlets, the shoots were in vitro rooted with an average number of 4.2 ± 0.4 roots per shoot in half-strength MS (½MS) medium supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized to field conditions with an 85% survival rate. Genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) analysis. The results showed that the monomorphic banding patterns of in vitro raised plantlets and their mother plant were similar, confirming its homogeneity and the reliability of the multiplication system. © 2021 Friends Science Publishers

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Plant regeneration through somatic embryogenesis from leaf and root explants ofRhynchostylis retusa(L.) Blume

Cited by 10 publications

References 0 publications

Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

Plant regeneration via direct somatic embryogenesis from leaf explants of Tolumnia Louise Elmore ‘Elsa’

Vi Nhân Giống Lan Đai Châu (Rhynchostylis Gigantea L.) Thông Qua Giai Đoạn Protocorm

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

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