Plant regeneration from the callus derived from root explants of Cucumis melo L. cv. Pusa sharbati

Kathal, Rekha; Bhatnagar, S. P.; Bhojwani, Sant S.

doi:10.1016/0168-9452(94)90230-5

Cited by 17 publications

(7 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further increase in the concentration of BAP did not improve the shoot size and number. Several workers reported similar results in cucumber (Kathal et al, 1988;Misra and Bhatnagar, 1995) and in Cucurbita interspecific hybrid cultivar "Shintoza" (Sarowar et al, 2003). Increased concentration of BAP reduced shoot length and promoted massive base callus.…”

Section: )supporting

confidence: 62%

In vitro propagation of Anchote (Coccinia abyssinica) (Lam.) Cogn.]

Bekele¹,

Abera²,

Getahun³

2013

Afr. J. Plant Sci.

View full text Add to dashboard Cite

Coccinia abyssinica (Lam.) Cogn.] (Cucurbitaceae) is a tuberous neutraceutical plant locally known as Anchote (Oromo language). A micropropagation protocol was developed for C. abyssinica. Seeds were planted ex vitro in a pot that contained different soil mix ratios, and two weeks old seedlings were used as a source of explants. Shoot tip and nodal explants were sterilized and cultured on full MS medium variously supplemented with 6-benzylaminopurine (BAP), indole acetic acid (IAA), naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) to produce adventitious shoot and rooting. High germination percentage (97%) was obtained from decoated seeds sown on soil mix ratios of 2:1:1 (sand, loam soil, coffee husk, respectively). Sodium hypochlorite at a concentration of 2.0% exposure of 5 min gave high percentages of survived nodal (79.43±0.6) and shoot tip (74.33±0.58) explants. 6-Benzylaminopurine (BAP) (3.0 µM) was found to be an optimum concentration for shoot induction, yielding 80% for nodal and 70% for shoot tip explants. The combination of BAP (3.0 µM) with IAA (0.5 µM) was obtained as optimum concentration yielding 13.4 and 11.03 shoots per explants for nodal and shoot tip respectively for shoot multiplication. Half strength MS medium supplemented with IBA (0.5 µM) and IAA (1.5 µM) yielded more than 90% rooting with optimum root number and length. For acclimatization, sterilized soil mix of 2:1:1 (top forest soil: coffee husk: sand) was optimized yielding 80% on transparent polyethylene plastic tube and 82.2% survived plantlets. This protocol provides a foundation for further studies to generate genetically improved C. abyssinica and related cucurbitaceous species.

show abstract

Section: )supporting

confidence: 62%

In vitro propagation of Anchote (Coccinia abyssinica) (Lam.) Cogn.]

Bekele¹,

Abera²,

Getahun³

2013

Afr. J. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…In this study, it was found that low concentration of cytokinin enhanced shoot multiplication. In other species many researchers reported the similar results (Kathal et al1988(Kathal et al , 1994Sing et al 1996 in Cucumis melo and Haque et al 2010 in pumpkin).…”

Section: Resultssupporting

confidence: 71%

Efficient Direct Organogenesis from Shoot Tips and Nodal Segments of Ash Gourd (Benincasa Hispida L.)

et al. 2014

View full text Add to dashboard Cite

Abstract:The effect of external application of phytohormone on explants viz., shoot tips and nodal segments of ash gourd (Benincasa hispida L.) was tested on the frequency of shoot and root induction. Shoot tips and nodal segments were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BAP, Kinetin), auxin (IBA, NAA) and gibberellic acid (GA 3 ) for multiple shoot formation and root induction. The highest number (up to 90%) of multiple shoot formation was obtained from the shoot tips in MS medium fortified with 1.5 mg/l BAP + 0.2 mg/l GA 3, where average number of shoots per culture was 5.55. In case of nodal segment, better response (up to 78%) for shoot multiplication was found in MS with 2.0 mg/l BAP + 0.2 mg/l GA 3. The concentration of 1.0 mg/l IBA was found to be most effective for root initiation in microshoot developed from both types of explants. Rooted plantlets were acclimatatized and established in sandy soil with good survival rate.Key words: Organogenesis, ash gourd, MS medium, shoot tips, nodal segments mvivskt PvjKz govi KvÊkxl© I ce© vsk G·c− v›U e¨envi K ‡i KvÛgy Kz j I gj Drcv` ‡b dvB ‡Uvni ‡gv ‡bi cÖ ‡qv ‡Mi e¨vwn¨K cÖ fve cix ¶v Kiv n ‡q ‡Q| eûgy Kz j Ges gj Rb¥vevi D ‡Ï ‡k¨ KvÊkxl© Ges ce© vsk¸ ‡jv GgGm wgwWqv ‡gi mv ‡_ wewfboe Nb ‡Z¡ i I wgkÖ ‡Yi mvB ‡UvKvBwbÝ (weGwc I KvB ‡bwUb), Aw·b (AvBweG I GbGG) Ges wR ‡eª wjK GwmW (wRG 3 ) mn ‡hv ‡M KvjPvi Kiv n ‡q ‡Q| 1.5 wgwjMÖ vg/wjUvi weGwc + 0.2 wgwjMÖ vg /wjUvi wRG 3 ni ‡gvb¸wj GgGm wgwWqv ‡gi g ‡a¨ e¨envi K ‡i KvÊkxl© †_ ‡K me ‡P ‡q †ewk (90% ch© šÍ ) eû-KvÛgy Kz j Drcv`b m¤¢ e n ‡q ‡Q Ges †hLv ‡b KvjPvi cÖ wZ gy Kz j msL¨v `u vwo ‡q ‡Q 5.55| ce© vs ‡ki † ¶ ‡Î KvÛgy Kz j Drcv` ‡bi Rb¨ 2.0 wgwjMÖ vg/wjUvi weGwc + 0.2 wgwjMÖ vg/wjUvi wRG 3 e¨envi K ‡i fvj dj cvIqv †M ‡Q (78% ch© šÍ )| Dfq ai ‡bi G·c− v ‡›Ui † ¶ ‡Î 1.0 wgwjMÖ vg/ wjUvi Nb ‡Z¡ i AvBweG e¨envi K ‡i ¶zª KvÛgy Kz j †_ ‡K me© vwaK gj Drcv`b cwijw ¶Z n ‡q ‡Q| wkKo MRv ‡bv AYy Pviv¸ ‡jv ‡K evjy hy ³ gvwU ‡Z Lvc LvIqv ‡bv Ges ¯' vqxKiY Kiv n ‡q ‡Q| G ‡`i †eu ‡P _vKvi nvi h ‡_ó fv ‡jv|

show abstract

“…Regeneration (Table 2) can also occur indirectly through callus. Regeneration through callus induction can be achieved using cotyledon (Moreno et al, 1985)t hypocotyl (Kathal et ai., 1986) and root (Kathal et al, 1994) explants. In each case, the age of the donor explant is critical.…”

Section: Regeneration By Organogenesismentioning

confidence: 99%

“…In each case, the age of the donor explant is critical. For example~highest growth callus and nodule formation is obtained with roots excised from 21-day..oId seedlings, while no nodule development occurs in 7-or 3S..day-old seedling root segments (Kathal et al, 1994).…”

Section: Regeneration By Organogenesismentioning

confidence: 99%