Melon Biotechnology

Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.)

“…To date, transgenic plants of C. sativus (Truson, Simpson, and Shahin 1986;Chee 1990), C. melo (Guis et al 1998), Cucurbita maxima and C. pepo (Pang et al 2000), and C. lanatus (Choi, Soh, and Kim 1994) were released. Various methodologies, including Agrobacterium rhizagenes and A. tumefaciensmediated transformation via somatic embryogenesis and organogenesis and biolistic transformation, have been used for production of these plants.…”

“…Various Agrobacterium-mediated and particle-bombardment transformation procedures have reported in melon (reviewed by Guis et al 1998), and Agrobacterium-mediated methods have been practically used for producing transgenic melon plants. The first successful Agrobacteriummediated transformation event produced conferred kanamycin resistance in melon by introducing the NPT-II gene (Fang and Grumet 1990).…”

“…A second problem was the low overall frequency of transformation events because of the presence of escapes (Guis et al 1998). In initial studies, transgenic plants were generated via adventitious-shoot organogenesis.…”

Research tools for functional genomics in melon (<i>Cucumis melo</i> L.): Current status and prospects

“…Various transformation procedures have been reported for melon (reviewed by Guis et al 1998), and an Agrobacteriummediated procedure has been found to be practical for the production of transgenic melon plants. The first successful Agrobacterium-mediated transformation conferred kanamycin resistance to melon by the introduction of the NPT-II (Fang and Grumet 1990) and GUS genes (Dong et al 1991;Valles and Lasa 1994).…”

“…In response, Guis et al (2000) developed a simple and efficient regeneration system that facilitated the production of diploid transformants at a high rate. Second, the frequency of transformation events was low due to the occurrence of "escapes" (Guis et al 1998). In initial studies, transgenic plants were generated via adventitious shoot organogenesis.…”