Plant regeneration from embryogenic suspension cultures of oil palm (Elaeis guineensis Jacq.)

Touchet, B. De; Duval, Yves; Pannetier, Catherine

doi:10.1007/bf00234588

Cited by 81 publications

(42 citation statements)

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“…Numerous secondary metabolites of medicinal uses were detected in cell suspension cultures of various plant species (Preil, 2005;Wilken et al, 2005). Several reports have been published on the establishment of cell suspensions of African oil palm (Elaeis guineensis) (de Touchet et al 1991;Teixeira et al, 1995;Aberlenc-Bertossi et al, 1999). Under the best conditions, the initial weight of cells increased about 4-fold in 1 month (de Touchet et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

“…Several reports have been published on the establishment of cell suspensions of African oil palm (Elaeis guineensis) (de Touchet et al 1991;Teixeira et al, 1995;Aberlenc-Bertossi et al, 1999). Under the best conditions, the initial weight of cells increased about 4-fold in 1 month (de Touchet et al, 1991). Similarly, many researchers carried out work on cell cultures of different date palm cultivars including cv.…”

Section: Introductionmentioning

confidence: 99%

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A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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Tissue culture techniques enable mass propagation of elite cultivars of date palm (Phoenix dactylifera L.). The main limitations for date palm in vitro multiplication are the low rates achieved using solidified medium and the long period needed to produce acclimatized plantlets. This research focuses on the comparison between different culture types and plant growth regulator (PGR) combinations on callus growth and somatic embryo formation of cv. Zaghlool. For callus growth, 200 mg friable embryogenic callus dispensed in Rasotherm and Phytacon flasks containing 200 ml liquid (100 rotations per minute) and in temporary immersion system, RITA ® bioreactor (5 min immersion every 12 h), were compared with cultures grown on 200 ml solidified medium. For somatic embryo formation, 500 mg of friable embryogenic callus grown in Erlenmeyer flasks filled with 50 ml liquid or solid MS medium. The medium was supplemented with 0.1 mg l -1 Naphthaleneacetic acid (NAA), 1.5 g l -1 activated charcoal (AC) with or without 0.05 mg l -1 6-Benzyl amino purine (BAP) compared to PGR-free medium. Results proved that cell suspension cultures produced the highest callus fresh mass as compared to the other systems tested, and the callus fresh mass reached 4 g after 16 weeks. The temporary immersion system did not significantly enhance the fresh mass of callus compared to the solidified medium. For somatic embryo induction, the number of somatic embryos increased in cell suspensions 6-16 fold compared to the solidified medium. Using liquid MS medium enriched with 0.1 mg l -1 NAA and 1.5 g l -1 AC gave rise to the highest number of somatic embryos formed from 500 mg initial callus: 160 embryos. The number of somatic embryos was also affected by the callus source. The calli induced from leaflet segments excised from converted somatic embryos resulted in a lower somatic embryo number than those of shoot tip origin with about 60 somatic embryos per 500 mg callus. The formation of somatic embryos using liquid medium required only 6 weeks, thus considerably reducing the previously reported period of 18 weeks which is required for somatic embryo formation using solidified media.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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“…Sistem kultur cair menyediakan kondisi kultur yang lebih seragam, penggantian medium lebih mudah dan dapat digunakan wadah yang lebih besar daripada kultur padat. Penggunaan medium cair mulai banyak dikembangkan untuk kultur in vitro kelapa sawit dengan tujuan meningkatkan laju pertumbuhan dan keseragaman kalus serta embrio somatik (de Touchet et al, 1991;Duval et al, 1993;Sumaryono et al, 1994;Teixeira et al, 1995;Ginting & Fatmawati, 1997;Tahardi, 1998).…”

Section: Pendahuluanunclassified

“…Pada medium padat, laju pertumbuhan kalus dan embrio somatik dianggap lebih rendah dibandingkan pada medium cair (de Touchet et al, 1991;Duval et al, 1993;Tahardi, 1998;Tarmizi et al, 2003;Soh et al, 2006). Di samping itu, kultur cair digunakan untuk meningkatkan keseragaman embrio somatik dan meningkatkan skala produksi.…”

Section: Pematangan Dan Perkecambahan Embrio Somatikunclassified

Pertumbuhan dan perkembangan kalus embriogenik dan embrio somatik kelapa sawit (Elaeis guineensis Jacq.) pada sistem perendaman sesaat Growth and differentiation of embryogenic callus and somatic embryos of oil palm (Elaeis guineensis Jacq.) in a temporary immersion system

SUMARYONO¹,

RIYADI²,

KAS³

et al. 2016

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SummaryIn temporary immersion system (TIS),plant materials are exposed to the medium fora short time, therefore they are more exposedto the air and a lack of oxygen frequentlyexperienced by a liquid culture can be avoided.This experiment was conducted to determinethe procedure for callus proliferation up tosomatic embryo germination of oil palm(Elaeis guineensis Jacq.) in TIS culture.Embryogenic calli of oil palm clone MK 638from Marihat Research Institute were culturedon solid medium in the dark culture room andthen used as materials for TIS. Immersion timefor all cultures was three minutes every sixhours. Callus proliferation was conducted inDF liquid culture with 5 mg/L 2,4-D and0.1 mg/L kinetin with transfer interval of 4, 6and 8 weeks. The treatments for somaticembryo maturation were kinetin and ABA,whereas for somatic embryo germination wasIBA, kinetin and GA 3 . The results show thatthe best transfer interval for callus proli-feration was four weeks. In this treatment therelative growth rate of callus was0.38 g/g/week. Somatic embryo initiation fromthe callus was done in DF mediumsupplemented with 1 mg/L 2,4-D and 0.1 mg/Lkinetin. The percentage of somatic embryowas 80% based on biomass fresh weight afterthe fourth subculture. The addition of 0.5 mg/Lkinetin and 0.05 mg/L ABA improved somaticembryo maturation of oil palm; the averagenumber of somatic embryos at advanced stages(torpedo and cotyledonary) was 16.3 embryosper flask. The addition of 2 mg/L IBA and0.5 mg/L kinetin in DF medium with half-strength macro-salt enhanced significantly thegermi-nation of somatic embryos. GA 3 at0.1 mg/L increased the total number ofgerminants.RingkasanPada sistem perendaman sesaat (SPS),bahan tanam hanya terpapar sebentar dalammedium sehingga paparan dengan udara lebihlama dan kekurangan oksigen yang seringterjadi pada kultur cair dapat diatasi. Penelitianini bertujuan menetapkan prosedur untukperbanyakan kalus embriogenik sampai denganperkecambahan embrio somatik kelapa sawit(Elaeis guineensis Jacq.) dalam kultur SPS.Kalus embriogenik kelapa sawit klon MK 638yang diperoleh dari Balai Penelitian Marihatdiperbanyak pada medium padat di ruang gelapyang kemudian digunakan sebagai bahan untukkultur cair SPS. Lama perendaman semuakultur di SPS diatur tiga menit denganfrekuensi setiap enam jam. Perbanyakan kalusdalam medium cair DF dengan 2,4-D 5 mg/Ldan kinetin 0,1 mg/L dilaksanakan denganinterval subkultur 4, 6 dan 8 minggu.Perlakuan pematangan embrio somatik adalahkinetin dan ABA sedangkan perlakuan untukperkecambahan embrio somatik adalah IBA,kinetin dan GA 3 . Hasil penelitian menunjuk-kan bahwa untuk proliferasi kalus embriogenikkelapa sawit, interval subkultur terbaik adalahempat minggu. Pada perlakuan ini laju tumbuhrelatif kalus mencapai 0,38 g/g/minggu.Inisiasi embrio somatik dari kalus dilakukanpada medium DF ditambah 2,4-D 1 mg/L dankinetin 0,1 mg/L. Persentase embrio somatikmencapai 80% dari total bobot basah biomassasetelah subkultur keempat. Penambahan kinetin0,5 mg/L dan ABA 0,05 mg/L meningkatkanpematangan embrio somatik kelapa sawit; rata-rata jumlah embrio somatik fase lanjut (torpedodan kotiledon) adalah 16,3 embrio per bejana.Penambahan IBA 2 mg/L dan kinetin 0,5 mg/Lpada medium DF dengan setengah garammakro meningkatkan perkecambahan embriosomatik secara nyata. GA 3 0,1 mg/L mening-katkan jumlah kecambah yang terbentuk.

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“…In the case of E. guineensis, several types of explants were already used to initiate somatic embryogenesis process: Mature or immature zygotic embryos (Balzon et al, 2013;Silva et al, 2014), cell suspensions from immature leaf segments (De Touchet et al, 1991), leaf segments (Pádua et al, 2013) and immature male inflorescence (Jayanthi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

Bonetti¹,

Nesi²,

Quisen³

et al. 2016

Afr. J. Biotechnol.

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Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were compared (Y3 and modified MS), and several growth regulators (2,4-D, Picloram, BA, 2iP), putrescine and activated charcoal (AC) were used during the different stages. Somatic embryos (SE) were obtained from ZE-derived embryogenic masses cultured on Y3 medium with or without 2,4-D (9 µM) + 1000 µM putrescine and 40% were converted into seedlings in the Y3 medium supplemented with 2 g.L -¹ AC and without growth regulators, under light. SE developed in 49 to 53% TCL-derived calli and 50% of them were converted into seedlings in the same conditions described for ZE calli. The yield of both processes was compared, showing the superiority of TCL explants for SE production.

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Plant regeneration from embryogenic suspension cultures of oil palm (Elaeis guineensis Jacq.)

Cited by 81 publications

References 11 publications

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Pertumbuhan dan perkembangan kalus embriogenik dan embrio somatik kelapa sawit (Elaeis guineensis Jacq.) pada sistem perendaman sesaat Growth and differentiation of embryogenic callus and somatic embryos of oil palm (Elaeis guineensis Jacq.) in a temporary immersion system

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

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