Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

Bonetti, K. A. P.; Nesi, J.; Quisen, R. C.; Quoirin, Marguerite

doi:10.5897/ajb2016.15482

Cited by 5 publications

(5 citation statements)

References 29 publications

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“…Several studies has also been reported regarding the usage of 2.4-D in micropropagation of oil palm. The usage of zygotic embryos as explants sources produced the highest somatic embryos formation from callus grown on Y3 medium without or low concentration of 2.4-D [14]. The induction of somatic embryos in immature oil palm inflorescences of the pisifera palm occurred at a concentration of 500 μM 2,4-D [15].…”

Section: The Effect Of 24-d and Naa On Somatic Embryos Formationmentioning

confidence: 99%

Evaluation of 2.4-D and NAA Concentrations for Callus and Somatic Embryos Formation in Oil Palm

Reflini¹,

Production²

2017

JOAAT

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The evaluation of 2.4-D and NAA concentrations for oil palm micropropagation were conducted in this study. Callus was initiated from immature leaflet of ten oil palm trees. Callus formation began two months after culture and increased at the subsequent subcultures. On average, the best callus induction rate was obtained in a culture medium without 2.4-D in combination with 6 mg/L of NAA. After 11 months of culture, somatic embryos were found mostly from nodular aggregate and nodular friable callus. At the end of culture time, the results showed that 6 mg/L of NAA slightly increase somatic embryos formation by the addition of 0.5 mg/L 2.4-D in both explants and callus culture. When 2.4-D was only added during callus culture, the formation of somatic embryos was low. Different results were shown in treatment with 10 mg/L of NAA. The formation of somatic embryos were much better by the addition of 0.5 mg/L 2.4-D only during callus culture. When 0.5 mg/L 2.4-D was added during explants and callus culture, the formation of somatic embryos were very low. In general, treatment of 10 mg/L NAA added with 0.5 mg/L 2.4-D during callus culture was the best media for oil palm propagation.

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Section: The Effect Of 24-d and Naa On Somatic Embryos Formationmentioning

confidence: 99%

Evaluation of 2.4-D and NAA Concentrations for Callus and Somatic Embryos Formation in Oil Palm

Reflini¹,

Production²

2017

JOAAT

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“…This callogenesis frequency of OxG hybrid was comparable to that of E. guineensis at 15% [8] or 11-20% [12] and another OxG hybrid at 11.25% [7]. Callogenesis frequency of OxG hybrids using zygotic embryos was very high at 77.9-95.3% after 90 days on Brazilian OxG hybrids [4] and at 10-60% after 6 months on other Brazilian OxG hybrids [6]. Activated charcoal at 0.25% was used to reduce oxidation in the culture of zygotic embryos of OxG hybrids [13].…”

Section: Callus Initiationmentioning

confidence: 99%

“…In vitro propagation of OxG hybrids through somatic embryogenesis (SE) has been reported mostly using zygotic embryos as explants [4,5,6], but SE in OxG hybrids from young leaf explants has been reported only by Ginting et al [7] using one OxG hybrid on solid media. However, SE with young leaves as explants has been widely used in E. guineensis [8].…”

Section: Introductionmentioning

confidence: 99%

Embryogenic callus initiation from leaf explants of Elaeis oleifera x Elaeis guineensis (OxG) hybrids

Sumaryono¹,

Riyadi²,

Saptari³

et al. 2018

IOP Conf. Ser.: Earth Environ. Sci.

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Abstract. Elaeis oleifera x Elaeis guineensis (OxG) hybrid is an oil palm interspecific crossbreed to produce a variety with better oil quality, slow stem growth, and resistance to bud rot disease. However, one of the problems in OxG hybrid breeding program is an inefficiency in the conventional propagation method, while the protocol for OxG hybrid tissue culture has not been established yet in Indonesia. Somatic embryogenesis (SE) using a temporary immersion system (TIS) will be used for OxG clonal propagation as it has been established successfully in E. guineensis. Explants were young leaves derived from genotypes of selected F1 and backcross (BC) of OxG hybrids where the E. oleifera was originated in Brazil and Suriname. Callus initiation used eight media with various compositions of minerals, hormones, and active charcoal. Initial callus emerged in 8-10 weeks after culture. The average of callogenesis frequency was 3.49% in F1 and 5.59% in BC hybrid explants, with the highest at 10.73% occurred in BC Brazil. Callogenesis of OxG hybrid showed a high potency as callogenesis of E. guineensis. The callus had nodular structure which was potentially embryogenic. After 3 cultures, some of the embryogenic calli were transferred to TIS for somatic embryo induction.

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“…Several studies have shown that protocols for plant regeneration by SE in tree species are challenging for the mass propagation of these plants [28]. Several strategies have been used to develop protocols for obtaining somatic embryos from TCLs in tree species [29,30]. Thus, there is a need to increase knowledge of SE in each tree species to increase the availability of plants for use in reforestation programs.…”

Section: Summary Of Tcl Applications On Tree Speciesmentioning

confidence: 99%

Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

Sharma,

Magotra,

Chourasia

et al. 2023

Forests

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An increased dependency on plant-based resources for food, shelter, and medicinal usage has increased their sustainable and unsustainable exploitation. To use this resource sustainably, plant tissue culture (PTC) is one important technology. Among different PTC techniques, thin cell layer (TCL) technology is a relatively simple and easily adaptable technique for in vitro cultures of plants. This technique uses small explants about 0.5–2 mm in thickness excised from different plant organs. It has been successfully used in the large-scale propagation of vegetables, legumes, and plants with medicinal benefits. TCL technology has proven to be effective in stimulating various organogenic responses when combined with various new methods such as nanotechnology or microtome-based explantation, especially in tree species. It is considered an important tool in plant biotechnology. Although the morphogenetic response per explant is usually higher in conventional explants, the appropriate use of plant growth regulators and geometric factors in TCL has the potential to make it more efficient and beneficial. This article provides an overview of the concept of TCL as applied to different plant species, particularly trees, since there are few, if any, summaries of TCL technology, especially in trees. This review will certainly revitalize this important technology so that it can be used effectively for successful mass propagation in the field of plant tissue culture.

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Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

Cited by 5 publications

References 29 publications

Evaluation of 2.4-D and NAA Concentrations for Callus and Somatic Embryos Formation in Oil Palm

Evaluation of 2.4-D and NAA Concentrations for Callus and Somatic Embryos Formation in Oil Palm

Embryogenic callus initiation from leaf explants of Elaeis oleifera x Elaeis guineensis (OxG) hybrids

Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

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