Plant regeneration from cell suspension cultures of Vigna aconitifolia

A b s t r a c tSomatic embryogenesis and plant regeneration were induced from immature embryonal axes and immature cotyledons of peanut (Arachis hypogaea L. fastigata type cv JLM-1). Influence of different auxins, cytokinins and sugars on somatic embryogenesis from immature cotyledon explants was also investigated. Among the different auxins tested, 2,4-dichlorophenoxyacetic acid (2,4-D) was most effective, producing the highest frequency of responding cultures and highest average number of somatic embryos per responding culture, while dicamba, picloram, indolepropionic acid, a-naphthaleneacetic acid, 2,4,5-trichlorophenoxypropionic acid and a-naphthoxyacetic acid were also effective for embryogenesis. Indolebutyric acid, indoleacetic acid, p-chlorophenoxyacetic acid and trichlorophenoxyacetic acid were not beneficial. Among the four cytokinins tested, zeatin slightly enhanced the frequency of somatic embryogenesis, while kinetin, 6-y-y-dimethylallylaminopurine and benzyladenine were relatively inhibitory. Among the different carbon sources tested, sucrose was the best for embryo induction and at 6% sucrose the highest frequency of responding cultures and average number of somatic embryos per explant were obtained. For inducing embryogenesis from embryonal axes, 2,4-0 was more effective than picloram. Highest plant conversion frequency from somatic embryos was obtained in presence of dicamba or NAA and using cotyledon explants.

Section: Methodsmentioning

confidence: 99%

Somatic embryogenesis in peanut: Influence of growth regulators and sugars

Eapen

George

1993

“…Selfed chickpea ovules were used to investigate the role of (i) genotype, (ii) age of the ovule, (iii) size of the ovule and (iv) culture medium. The control medium used in the experiments was ML6 medium with 90 g l )1 sucrose (Kumar et al, 1988), 1 mg l )1 zeatin and 0.25 mg l )1 indole acetic acid (Mallikarjuna, 1999). Ovules were cultured in the light with a 16:8 h photoperiod at 25°C.…”

Section: Embryo Rescue In Ovulementioning

confidence: 99%

Embryo rescue and plant regeneration in vitro of selfed chickpea (Cicer arietinum L.) and its wild annual relatives

Clarke

Wilson

Kuo

et al. 2006

The main constraint to the transfer of desired traits into cultivated chickpea from wild Cicer relatives is the presence of post-zygotic barriers which result in abortion of the immature embryo following interspecific hybridisation. Rescue of hybrid embryos in vitro and regeneration of hybrid plantlets could allow chickpea breeders to transfer desirable traits from wild relatives of chickpea. The development of embryo rescue techniques using selfed chickpea and selfed wild relatives is being used as a first step to protocols for wide hybrids. Optical microscopy studies of embryogenesis, in both selfs and hybrids, identified deleterious changes in the fertilised hybrid seed as early as 2-4 days after pollination in some crosses. These observations suggest that the appropriate time to rescue chickpea Â C. bijugum hybrids is at the early globular stage of embryogenesis (2-7 days old), which requires the development of a complex tissue culture medium. In contrast hybrids between chickpea Â C. pinnatifidum abort later (up to 15-20 days old) at the heartshaped or torpedo stages, and are easier to rescue in vitro. Genotype also plays a significant role in the ability of immature selfed ovules to germinate in vitro. In this paper we report on the optimisation of protocols for rescueing immature embryos using selfed chickpea and its wild relatives in ovule, and subsequently to regenerate plantlets.

“…In this species detailed protocols have been worked out for regenerating plantlets from a number of somatic tissue explant [3,8,11], protoplast-derived cell clonies [25] and cell-suspension culture [15]. In addition, tissue culture studies have also been done in V. mungo [9]; V. unguieulata (syn.…”

Section: Introductionmentioning

confidence: 99%

Culture conditions effecting plant regeneration from cotyledons of Vigna radiata (L.) Wilczek

Gulati

Jaiwal

1990

Conditions for plant regeneration from excised cotyledons of Vigna radiata were studied. Complete plant developed from the uncallused proximal ends of cotyledons on Murashige & Skoog's (MS), Gamborg's (Bs) and 'C' (MS salts + Bsvitamins) basal media. The basal medium 'C' was found to be best for plant regeneration. Regeneration frequency, however, varied with genotype, size, orientation and age of explant and the different plant growth regulators combination in the medium. Addition of cytokinins induced callusing at the proximal ends of cotyledons followed by multiple shoot formation. Out of 6-benzyl aminopurine (BAP), kinetin (KIN), N (A 2 isopentyl) adenine (2iP) and adenine sulphate (AS), only BAP and KIN were found to be more effective in enhancing the frequency of shoot regeneration. BAP at 1 x 10-SM induced maximum (60%) shoot regeneration whereas maximum number of shoots (8 to 9 shoots) per explant was observed with 5 x 10 6 M BAP. Cotyledons excised from two-day old seedlings were most regenerative. The regenerative response of cotyledons decreased when sliced into two equal parts either longitudinally or transversely. Callusing and organogenic differentiation occured only if the petiolar end of cotyledons was in contact with medium. None of the tested treatments were effective in inducing shoot bud differentiation from subcultured callus. Well developed shoots rooted when incubated on half strength MS, MS and MS basal medium supplemented with IAA (5 x 10-6M). The rooted plants were transferred to pots and later established in the field with 60% success.