Culture conditions effecting plant regeneration from cotyledons of Vigna radiata (L.) Wilczek

Abstract

Conditions for plant regeneration from excised cotyledons of Vigna radiata were studied. Complete plant developed from the uncallused proximal ends of cotyledons on Murashige & Skoog's (MS), Gamborg's (Bs) and 'C' (MS salts + Bsvitamins) basal media. The basal medium 'C' was found to be best for plant regeneration. Regeneration frequency, however, varied with genotype, size, orientation and age of explant and the different plant growth regulators combination in the medium. Addition of cytokinins induced callusing at the proximal ends of cotyledons followed by multiple shoot formation. Out of 6-benzyl aminopurine (BAP), kinetin (KIN), N (A 2 isopentyl) adenine (2iP) and adenine sulphate (AS), only BAP and KIN were found to be more effective in enhancing the frequency of shoot regeneration. BAP at 1 x 10-SM induced maximum (60%) shoot regeneration whereas maximum number of shoots (8 to 9 shoots) per explant was observed with 5 x 10 6 M BAP. Cotyledons excised from two-day old seedlings were most regenerative. The regenerative response of cotyledons decreased when sliced into two equal parts either longitudinally or transversely. Callusing and organogenic differentiation occured only if the petiolar end of cotyledons was in contact with medium. None of the tested treatments were effective in inducing shoot bud differentiation from subcultured callus. Well developed shoots rooted when incubated on half strength MS, MS and MS basal medium supplemented with IAA (5 x 10-6M). The rooted plants were transferred to pots and later established in the field with 60% success.

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

“…Kinetin was less effective than BA in terms of bud proliferation as well as shoot bud induction, but exerted a beneficial effect on shoot elongation (> 3.36 cm). Promotion of shoot bud differentiation in combined use of BA and kinetin has been reported in Feronia limonia L. hypocotyl explants (Vyas et al 2005) and cotyledonary explants (Hossain et al 1994) as well as Vigna radiata L (Gulati and Jaiwal 1990). The use of either cytokinin separately or in synergistic combination led to a characteristic phenotype in vitro after 7 days of culture at the bud proliferation stage before subsequent subculture (Fig.…”

In vitro Regeneration of Recalcitrant Green Gram (Vigna radiata L. Wilczek) from Immature Cotyledons for Genetic Improvement

“…The maximum multiple shoot production was obtained from cotyledonary node when cultured in MS media fortified with 1.5 mg/l BAP and 1.0 mg/l kinetin. Shoot multiplication and propagation of green gram have also been reported by different workers using different explants (Sagare et al, 2015;Khatun et al, 2008;Vijayan et al, 2006;Gulati et al, 1990). Increased BAP concentration from 2.0 to 3.0 mg/l, the rate of shoot bud regeneration increased but the shoot growth was reduced.…”

Vigna radiata as a New Source for Biotransformation of Hydroquinone to Arbutin

“…Hydroquinone was dissolved in bi-distilled water (10 mg/ml) and administered aseptically through a 0.2 μm membrane filter to obtain final concentrations (6,8,10,15, and 20 mg/100 ml). Each experiment was carried out in three replicates.…”

“…13 In vitro cultures of Vigna radiata were reported earlier. [14][15][16][17] The present research investigated the biotransformation possibility of hydroquinone to arbutin for the first time. On the other hand, it evaluated its bioconversion capacity by adding elicitors like chitosan and methyl jasmonate to V. radiata cell culture.…”